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Coli, and purify rWHcAg under native condition by precipitation with saturated ammonium sulfate and CsCl gradient centrifugation; Immunize Balb/c mice with purified rWHcAg to prepare antiserum; Establish a competitive inhibition ELISA method to detect WHcAb in serum of Chinese marmots.

原核表达重组WHcAg，氯化铯密度梯度离心获得非变性的纯化蛋白；用该纯化蛋白免疫Balb/c小鼠制备多克隆抗体；建立竞争抑制ELISA方法用于检测旱獭血清中的抗－WHc。

Specific aim ground elaborated bedding face to accumulate the construction technology of structure of big gradient inclined roofing, content includes to undertake to scaffold concentrated to reinforcing steel bar problem adopts much way to coordinate deepening designing; solve; to use obligate level to irrigate the; of match plate system that builds groove, section to seal a model to consider to implement the technical measure that improves concrete construction performance and constituent measure.

有针对性地阐述了大面积大坡度斜屋面结构的施工技术，内容包括对脚手架进行深化设计；对钢筋密集新问题采取多途径协调解决；采用预留水平浇筑槽、分段封模的双面模板体系；探究实施提高混凝土施工性能的技术办法及组织办法。

In this paper, the following progress has been discussed:(1) the improved conjugate gradient algorithm which combines the inversion equation;(2) the formulation for velvet gene at all directions and the approximation method of Jacobian matrics based on disturbance method;(3) the model is adjusted by analyzing the influence of damp coefficient and correction factor to inversion result ;(4) the influence of damp coefficient to the resolution of inversion is studied. to some degree, we improved the problem of "barycenter ascending shift" to electric anomalous body in 3-D resistivity inversion.

本文主要讨论了以下几个方面的内容：（1）结合反演方程式改进的共轭梯度算法；（2）基于扰动法的线源Jacobi矩阵的近似计算方法及光滑系数矩阵在各个方向上的光滑因子的计算公式；（3）通过分析阻尼系数与修改量校正量对反演结果的影响，提出了利用校正系数对模型修正量进行校正；（4）较为系统的讨论了阻尼系数λ对反演分辨能力的影响，较好的改善了三维电阻率反演中的电性异常体重心的&上漂&现象。

In order to adapt the light abrupt variation, we use a kind of variationdetection method to check the illuminance variation. Then carry on reclassification ofthe results of MOGs method with gradient information, avoiding a great deal ofbackground misclassified as foreground.

为适应光照变化提出一种变化检测方法，当检测到光照突变时，用梯度信息对混合高斯模型的分割结果进行校正，以避免大量背景误判为前景。

The wavefronts are reconstructed by the results of the measured wavefront gradient, and the wavefront aberration is obviously shown.

应用模式法，通过孔径斜率进行了波前重构，结果表明热射流使波前产生较强的随机畸变。

As a result, the model of low-frequency aberration induced by Nd:glass amplifiers is got. On the base of low-frequency aberration model some results are achieved such as the tendency of focal spot size changing as the RMS phase gradient changes and the characteristics for the scale length. 3. The simulation model of the beamline is set up and the propagating process is analyzed in the beamline. The correlation coefficient of two wavefronts is newly used to describe and estimate the adding result of them.

建立了求空间尺度的数学模型，并运用此模型对现有的放大片透射波前进行了分析，得出了多程系统中所用钕玻璃放大片引入的低频位相误差的空间尺度分布，并据此建立了模拟放大片低频位相误差的随机位相屏模型，在此基础上对焦斑大小随位相分布RMS梯度变化而变化的规律及空间尺度的特性进行了模拟研究； 3。

The building boundaries were then obtained by computing the watersheds of the modified gradient image.

该方法能够引入建筑物目标的特性同时克服分水岭变换固有的过分割缺陷。

Long with the program and technology to our company,and start to develop the High-gradient varistor.

此次研发的高梯度电阻片，采用独特的设计，特殊的生产工艺，新型优质材料制造。

In this paper, research works on the improvement of the voltage gradient and energy-handling capability of the ZnO varistor s are reviewed.

本文针对1000kV交流系统和±800kV直流系统中各种避雷器对安全可靠性、重量轻、体积小型化的要求，总结了国内外竞相开展的提高ZnO 压敏电阻片电位梯度和能量吸收能力的研究开发工作。

The morphology of turbidite channels is mainly controlled by topographic gradient, and the high tortuous channels mostly developed in gentle slopes.

浊积水道的形态主要受地形坡度控制，其中高弯度水道主要发育在缓坡地段。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。