查询词典 gradient method

METHODS: Mouse bilateral femur was sterilely isolated. BMSCs were harvested by the Percoll density gradient centrifugation, and purified and proliferated by the adherent method.

无菌分离小鼠双侧股骨，Percoll密度梯度离心法分离骨髓间充质干细胞，再用贴壁筛选法纯化扩增。

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

METHODS: Rat double lower limbs were isolated sterilely. Bone marrow was obtained by rinsing using low-glucose DMEM. BMSCs were isolated and purified by the whole bone marrow adherence method and density gradient centrifugation.

无菌条件下分离大鼠双下肢，低糖DMEM培养液冲出骨髓，分别运用全骨髓贴壁法和密度梯度离心法体外分离骨髓间充质干细胞，利用差速贴壁原理对细胞进行纯化扩增。

Objective To establish a new method for isolating serum β-lipoprotein subfraction s using second double gradient polyacrylamide gel electrophoresis.

目的建立一种分离血清β脂蛋白亚组分的新方法———二阶双梯度聚丙烯酰胺凝胶电泳法。

Ni-S-Co coated electrode was prepared on foam nickel matrix by means of gradient electrodepostion method by increasing sulphourea concentration in electrolyte.

以泡沫镍为基体，通过提高镀液中硫脲浓度的方法梯度电沉积制备了非晶态Ni-S-Co合金镀层电极。

Objective The discussion use serum-effusion albumin gradient to accumulate the fluidity archery target to the chest cavity to judge whether surpasses the tradition to infiltrate the transudate the classified method.

目的探讨使用血清-胸腔积液白蛋白梯度对胸腔积液性质的判断是否优于传统的渗漏出液的分类方法。

Objective The discussion use serum-effusion albumin gradient to accumulate the fluidity archery target to the chest cavity to judge whether surpasses the tradition to infiltrate the transudate the classified method.

苏州大学，基础医学，2009年，硕士目的探讨使用血清-胸腔积液白蛋白梯度对胸腔积液性质的判断是否优于传统的渗漏出液的分类方法。

Based on density functional theory with the generalized gradient approximation and a full-potential linearized augmented plane wave method, the calculation of the electronic structures and the magnetic properties on [Cuμ-1,3-N3](ClO4), where L is the tridentate Shiff base, has been performed.

基于广义梯度近似密度泛函和全势能线性缀加平面波方法，本文对聚铜络合物[Cuμ1，3-N3](ClO4)（其中L=tridentate Schiff base为三齿席夫基）的态密度和磁矩进行了计算。

Methods The method of sucrose gradient centrifugation was used to purify Human herpesvirus-6B(HHV-6B); and in order to make sure the range of positive samples collection, the viron was detected by the methods of PCR, Dot blotting, Western blotting.

采用蔗糖密度梯度离心法进行病毒纯化；通过PCR法、斑点杂交法、蛋白免疫印迹法对纯化样品中HHV-6B进行鉴定，以确定收集阳性样品的范围。

This principle eradicates local minima or invalid solutions caused by consistent reduction of the energy function via the usual gradient descent method.

利用这一理论，可以克服由于梯度下降法所导致的网络能量函数总是下降，从而使网络陷入局部极小值或不可行解的现象。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。