查询词典 gradient method

Traditional separation method: Several years ago, as a result of the equipment condition limit, the material particle DNA separation with the CsCl balanced equilibrium density centrifuge process, the idiomorphic become generally the gradient.

传统的分离方法：数年前，由于受设备条件限制，质粒DNA的分离一般用CsCl平衡等密度离心法，自形成梯度。

Methods The primary skeletal muscle satellite cells of New Zealand white rabbits were cultured with different adhesion time method and incontinuous Percoll density gradient centrif...

分别采用差速贴壁法和Percoll非连续密度梯度离心法进行新西兰兔肌卫星细胞的原代培养并进行纯化。

A convenient and rapid method was developed to prepare isocratic and concentration gradient linear polyacrylamide columns, and poly dA(40-60) and double string DNA were separated efficiently with an efficiency of 6 × 106 plates/m.

提出&筛分能力&作为 CGE柱评价指标，对样品迁移行为随操作条件的变化规律进行考察，为毛细管凝胶电泳的理论研究和实验条件优化奠定了基础。

So to provide method guideline and experimental basis for studying and establishment of HPLC Fingerprint photometry of Caulis Herbs like leaves of Mangifera indica L.. MethodsWaters symmetry C18 (5 μm,4.6 mm×250 mm) column was applied with 0.1% phosphoric acid as mobile phase in a gradient mode.

目的通过考察3个品种芒果叶不同组织部位甲醇提取物的高效液相色谱指纹图谱，并对其HPLC指纹图谱进行比较，为研究和测定芒果叶等叶类药材的HPLC指纹图谱提供方法指导和实验依据。

Evolution pattern of solidification microstructure in the monophase Cu Ni alloy in the range of thermo dynamically supercooling of 63～292 K is investigated, and both the condition of supercooling degree and orientation mechanism and control method of directional crystalline growth in the melt under negative temperature gradient are analyzed.

研究了 6 3～ 2 92K热力学过冷度范围内，Cu Ni单相合金的凝固组织演化规律，分析了负温度梯度熔体中晶体定向生长的过冷度条件以及晶体生长取向机制与控制方法。结果表明：(1)在临界过冷度ΔT (≈ 10 6K)附近，晶粒细化过程受溶质扩散控制，在临界过冷度ΔT (≈ 2 0 0K)附近，晶粒细化过程受热扩散控制；(2 )在 6 3～ΔT K范围内，晶体的生长方向是 [10 0 ]，在 12 7～ΔT K范围内，晶体的生长方向可以是 [10 0 ]，也可以是

Through solving the nondivergent inviscid linear vorticity equation in cylindrical coordination of barotropic atmosphere with WKBJ method, the authors point out that one kind of wave, named vortex Rossby wave, which is similar to planetary wave, exists in hurricane, its formation is due to the gradient of vertical vorticity component of basic state.

作者中文名：钟科；康建伟；余清平摘要：文中从柱坐标系下的正压无辐散涡度方程出发，用WKBJ方法求解方程，发现在飓风中存在类似于行星罗斯贝波的涡旋罗斯贝波，这种波的形成主要是由于飓风中基态涡度垂直分量的径向梯度所决定。

Experimental materials include CO2 incubator,M199, fetal calf serum,Ⅳ collagenase, 40 g/L of paraform, 20 g/L of cidex,ECGF, mouse-anti-human FⅧ monoclonal antibody(anti-FⅧ-RAg), multi-marked immune analyser(1 420, WALLAC BLCTOR2), centrifuge, inverted microscope, and immunofluorescence microscope, etc.METHODS: Three-step gradient sieve method was used.

实验材料为CO2培养箱，M199，胎牛血清，Ⅳ型胶原酶，40 g/L多聚甲醛，20 g/L戊二醛，ECGF，鼠抗人第八因子单克隆抗体，多标记免疫分析系统(1 420，WALLAC BLCTOR2)，离心机，倒置显微镜，免疫荧光显微镜等。

ML of bone marrow was sterilely aspirated from the posterosuperior iliac spine of 36 healthy mongrel dogs. BMSCs were isolated and purified by Percoll density gradient centrifugation and cultured in vitro by adherence method.

选择健康杂种犬36只，无菌抽取犬骨髓液10 mL，经Percoll液密度梯度离心后贴壁法体外培养骨髓间充质干细胞，传2代后行5-氮胞苷诱导，5-溴脱氧尿苷标记备用。

The first order gradient information was obtained by the analytical method and preconditioned automatic differentiation.

在该方法中优化命题采用简约空间SQP算法求解，求解过程中需要的导数信息采用解析表达和预处理的自动微分技术求取。

Three dimensional functionally gradient materials ; Macro and meso scopic method ; Analytical model ; Particle reinforced ; Inter laminted stress analysis

三维梯度功能材料；宏细观方法；分析模型；颗粒增强；层间应力分析

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。