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Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证实。

Using conventional garnetbiotitemuscoviteplagioclasequartz geothermobarometry, pT conditions were estimated to be 590610 ℃ and 0.951.14 GPa for the Huilongsi Formation, and 630650 ℃ and 0.650.89 GPa for the Zuolaozhuang Formation, in agreement with lower and upper amphibolite facies, respectively.

运用传统的石榴石黑云母白云母斜长石石英地质温压计估算回龙寺组的变质pT区间为590~610 ℃、095~114 GPa,左老庄组为630~650 ℃、065~089 GPa,分别相当于低角闪岩相和高角闪岩相。

2Graduate School of Chinese Academy of Sciences, Beijing 100049, ChinaAbstract: Two sets of dehydrationmelting with a natural solid amphibolite(56vol.% hornblende, 43vol.% plagioclase), collected from the DabainengLeyu profile in the southern Ailaoshan orogenic belt, Honghe County, Yunnan Province, have been carried out in multianvil apparatus(YJ3000t and JL3600t) at 1035 GPa and 950 ℃ for 20300 h. One is keeping the temperature at 950 ℃ and the annealing time of 100 h, varying the pressure (1035 GPa). The other is keeping the pressure at 30 GPa and temperature at 950 ℃, varying the annealing time (20300 h). The products are inspected with microscope and electron probe.

利用YJ3000t和JL3600t多顶砧压力机,以哀牢山造山带南部红河县大白能-乐育剖面上的天然块状斜长角闪岩为初始样品,在950 ℃、10~35 GPa、恒温20~300 h条件下进行了两个系列的斜长角闪岩块状样品脱水部分熔融实验:(1)保持温度T=950 ℃,加热时间t=100 h不变,改变压力(10~35 GPa)的实验;(2)保持温度T=950 ℃,压力p=30 GPa不变,改变加热时间(20~300 h)的实验。

The results show that the interplanar crystal spacing and lattice index of Al-rich α phase reduce gradually with increasing pressure. When the pressure reaches from common pressure to 5 GPa , Al-rich α phase lies on the (200) and (220) crystal faces, the interplanar crystal spacing are reduced by 1.07% and 1.15%. From common pressure to 3 GPa , the microstructure of ZA27 alloy changes from thick dendrites to fine dendrites, and finally to granular. According to the effect of solute diffusion coefficient under super high pressure and the calculation of Scherrer formula, it can be seen that the crystal organization is fine within the bounds of certain pressure. This conclusion is consistent with the experiment results.

结果表明:随着压力的增加,晶面间距和晶格常数都逐渐减小,当压力达到5 GPa时,富铝α相在(200)与(220)晶面处,晶面间距较常压力时的分别减小1.07%和1.15%;在常压至3 GPa范围内,晶粒由粗大的树枝晶→细小的树枝晶→粒状、椭球状枝晶转变的趋势,通过压力对合金凝固溶质扩散系数的影响结合Scherrer公式计算分析其组织变化原因,得出在一定的压力范围内,随着压力的增大,晶粒尺寸逐渐变小,这一结论与实验结果相符。

Hence, the modulus of rigidity, G, of glulam was measured by vibration test method in order to realize the shear deflection effect. The E/G values of Japanese cedar, Taiwania, Douglas-fir, and southern pine glulam were ranged form 13.2 to 14.5, 14.6 to 16.6, 12.1 to 16.6 and 16.0 to 19.1, respectively. The pure MOE was also obtained. The Ep and MOR values of glulam were ranged from 10.6 to 13.4 GPa and 48.3 to 61.9 MPa for Japanese cedar, ranged from 12.1 to 15.0 GPa and 41.8 to 58.8 MPa for Taiwania, ranged from 10.5 to 16.4 GPa and 37.2 to 65.6 MPa for Douglas-fir, ranged from 14.5 to 20.6 GPa and 70.2 to 78.48 MPa for Southern pine, respectively.

而消除剪力挠曲影响,即可得集成材之抗弯弹性模数真实值与抗弯强度,依本研究配置所得之柳杉集成材之Ep值与MOR值分别为10.6-13.4 GPa与48.3-61.9 MPa;台湾杉集成材之Ep值与MOR值分别为12.1-15.0 GPa与41.8-58.8 MPa;花旗松集成材之Ep值与MOR值分别为10.5-16.4 GPa与37.2-65.6 MPa;南方松集成材之Ep值与MOR值分别为14.5-20.6 GPa与70.2-78.4 MPa。

Through geothermometry and geobarometry, the temperature and pressure conditions of the three types of amphibolites are calculated, gneissic amphibolites: 597.0℃, 0.536 GPa, banded amphibolites: 617.3℃, 0.455 GPa, mylonitic amphibolites: 558.8℃, 0.514 GPa.

通过地质温压计计算,3种斜长角闪岩及其原岩的形成与变形温压条件具有从升温升压经降温升压到降温降压的变化规律(片麻状斜长角闪岩:597.0℃,0.536 GPa;条带状斜长角闪岩:617.3℃,0.455 GPa;糜棱状斜长角闪岩:558.8℃,0.514 GPa)。

When China became a WTO Member in 2002, it committed to join the GPA "as soon as possible." In 2007, China began GPA negotiations by tabling an initial offer.

2002年成为WTO成员时,中国承诺&尽快&加入GPA.2007年中国开启了加入GPA的谈判,并提交了一份初始出价。

Noting China's intention to become a Party to the GPA, some members of the Working Party stated that China should, upon accession, become an observer to the GPA, and should initiate negotiations for membership in the Agreement by tabling an Appendix 1 offer within two years of accession.

注意到中国有意成为GPA参加方,一些工作组成员表示,中国应自加入时起成为GPA观察员,并应在加入后2年内通过提交附录1出价,开始加入该协定的谈判。

The moduli of nacres in parallel and oblique directions with respect to the growing trace are 60.3 GPa and 56.7 GPa, while in perpendicular direction the elastic modulus is 48 GPa.

结果表明,在平行和倾斜于生长纹路方向上弹性模量的平均值分别为60.3 GPa和56.7 GPa,而垂直于纹路方向的为48 GPa,呈现出各向异性。

After standard fire exposure test, the bending properties of glulam decreased with the fire exposure time increasing. The values of MOE and MOR decreased respectively from 4.1 GPa to 2.5-3.8 GPa and 48.2 MPa to 27.1-36.6 MPa for China fir glulam, from 5.3 GPa to 3.7-4.6 GPa and 54.8 MPa to 23.0-39.4 MPa for Japanese cedar glulam, from 5.3 GPa to 3.4-4.7 GPa and 51.4 MPa to 21.8-38.6 MPa for Taiwania glulam, from 7.7 GPa to 6.6- 6.9 GPa and 62.7 MPa to 33.2-40.8 MPa for Douglas-fir glulam, from 6.0 GPa to 4.4-5.3 GPa and 58.9 MPa to 26.4-42.1 MPa for Southern pine glulam.

经燃烧试验后,集成材之力学性质上,集成材之抗弯强度会随燃烧时间之增加而降低,其中,杉木集成材之MOE值由4.1 GPa降至2.5 - 3.8 GPa,MOR值由48.2 MPa降至27.1 - 36.6 MPa;柳杉集成材之MOE值由5.3 GPa降至3.7 - 4.6 GPa,MOR值由54.8 MPa降至23.0 - 39.4 MPa;台湾杉集成材之MOE值由5.3 GPa降至3.4 - 4.7 GPa,MOR值由51.4 MPa降至21.8 - 38.6 MPa;花旗松集成材之MOE值由7.7 GPa降至6.6 - 6.9 GPa,MOR值由62.7 MPa降至33.2 - 40.8 MPa;南方松集成材之MOE值由6.0 GPa降至4.4 - 5.3 GPa,MOR值由58.9 MPa降至26.4 - 42.1 MPa。

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