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Therefore it's necessary to contol the concentration of TW80 to use it in the remediation of pyrene contaminated soil by AMF and corn integrated systerm.4. The AMF community and the single species derived from rock oil-contaminated soil all can be combined with corn and promote the remidation of pyrene contaminated soil , the original AMF community has obvious advantages and behave best.The residual pyrene concentration of soil in the treatments with the mixed AMF community M4 (Glomus intraradices, Glomus mosseae, Glomus etunicatum and Acaulospora tuberculata) and corn integrated systerm got 0.12mg/kg,0.54mg/kg and 3.92mg/kg respectively in 1mg/kg, 10mg/kg and 50 mg/kg pyrene treatments,decreased 32.6%,58.6% and 42.2% respectively than that of the treatment of M1,decreased 28.1%,59.9% and 36.6% than that of M2(G.intradices), decreased 2.7%,55% and 23.5% than that of M3 (G.intradices + G.mossea).5.Large scale reproduce overall of AMF community which coming from rud oil-contaminated soil in the absence of the original conditions will change the quantiy,composition and structure of the AMF population and may has negative impact on it's function in the remedition of soil pyrene contamination.

因此，在植物与AMF联合修复土壤芘污染中使用TW80应该注意控制TW80的浓度。4、从石油污染土壤中扩繁出的AMF混合菌群及其中的单一菌种与玉米联合均能促进土壤芘污染的修复，原始混合菌群具有明显的种群优势，其与玉米联合修复土壤芘污染的效果最佳。1mg/kg、10mg/kg和50mg/kg芘处理土壤中，玉米接种混合菌种M4(Glomus intraradices， Glomus mosseae， Glomus etunicatum和Arcaulospora tuberculata)处理土壤的芘残留浓度分别为0.12mg/kg，0.54mg/kg和3.92mg/kg，分别比接种单一菌种M1处理降低了32.6%，58.6%和42.2%，比接种单一菌种M2(G.intradices)处理降低了28.1%，59.9%和36.6%，比接种复合菌种M3(G.intradices+G.mossea)处理降低了2.7%，55%和23.5%。5、从石油污染土壤中扩繁出的混合AMF菌群在无原始条件下的整体扩繁会改变菌群的组成及其结构，并会对菌群与玉米联合修复土壤芘污染的功能产生不利影响。

Dis- Fly away lonely souls into the endless sky so far away When you're all alone Feeling down and lonely Tell me what you see deep inside your soul You only know who you are So don't be afraid Just open your heart to me You will spread your wings like an eagle Learning how to fly in the sky Just like the sun rises after the rain you'll shine in the air Take off your mask and be true to your heart trust yourself and fly away Into the light of joy and truth Where there's a grief there will always be a hope For a place where we can be who we wanna be Your tears may fade away But always keep the faith and live tomorrow Break through the night away into a brand new day Life's just begun Yeah, feelin' the melody of eternity It has the power to set us free Travlin' through the galaxy with only space around Follow the suns peaceful sound It's time for us to end this illusion Fightin' ourselves will only cause confusion Shinin' light is the key of life Let's all get together and make this better I don't know how but there has got to be a way There are times when I Wish I could just disapear Far from this place of pain and hurt If only I could be gone There's something in your eyes Pulls me back from running away Oh, yes it's hard sometimes But now I'm strong enough to tell the sadness goodbye Just like the moon fills the night with the stars I'll dance in the air Seek in the darkness there's nothing to fear I will stand and fly away Into the night I'll find the truth The pain never ends but there's always be a hope To be free eternally in this galaxy To live another day the sun will show the way and I will follow Break through the night away into a brand new day My journey goes on Into the light of joy and truth Where there's a grief there will always be a hope For a place where we can be who we want to be Your tears may fade away But always keep the faith and live tomorrow Break through the night away into a brand new day Your journey's begun

阴间的神-飞孤单的灵魂进入在如此远处的永无止境的天空之远之内当你全部很孤独的时候感觉下来和孤单的告诉我你在你的灵魂里见到起来深的你只知道你是谁如此不害怕仅仅对我开放你的心你将会像一只鹰一样传布你的翅膀学问该如何在天空中飞仅仅在雨之后喜欢太阳上升你将会在空气中发亮脱掉你的假面具而且忠于你的心信赖你自己和飞行之远进入欢喜和事实的光之内哪里总是有伤心事在那里意志是一个希望对于我们能是的一个地方我们想要当谁你的泪滴可能褪色离开但是总是保存信心和活的明天进入商标新的日子之内夜晚之久突破仅仅被开始的生活是的，feelin'永恒的曲子资讯科技有力量释放我们 Travlin'经过银河由于只在附近隔开跟随太阳和平的声音资讯科技是我们结束这一个幻影的时候 Fightin'我们自己只将会引起混乱 Shinin'光是生活的钥匙全部让我们聚在一起而且使这变成更好我不知道如何除了那里之外已经开始是一个方法有时代此时我希望我可以正直的 disapear 离痛苦和伤害的这一个地方很远如果只有我可能不见了某事在你的眼睛中从逃走把我拉回来哦，是的资讯科技努力地有时是但是现在我够强壮来告诉悲哀再见仅仅喜欢，月亮将夜晚装满星我将在空气中跳舞黑暗的找寻没有东西害怕，我将会站而且飞离开进入夜晚之内我将找事实痛苦从不结束但是总是有是一个希望在这银河中是自由地永恒地在另外的一个日子居住太阳将会表示方式而且我将会跟随进入商标新的日子之内夜晚之久突破我的旅程继续进入欢喜和事实的光之内哪里总是有伤心事在那里意志是一个希望对于我们能是的一个地方我们想要当谁你的泪滴可能褪色离开但是总是保存信心和活的明天进入商标新的日子之内夜晚之久突破你的被开始的旅程

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

He went downstream,and arrived at the river mouth to the sea.Suddenly,the god of the North Sea,whose name was Biennia Ruo,appeared before his eyes.With a smile,Beihai Ruo was welcoming He Bo to the North sea.He looked ahead,and saw that the North Sea,with its vast expanse of water,was boundless.With a dull look in his eyes,He Bo stood there for a while.Finally he said to Beihai Ruo with deep feeling,As the common saying goes,some people go so far as to think that they are more knowledgeable than anybody else when they have got some knowledge.

河伯顺流来到黄河的入海口，，突然眼前一亮，海神北海若正笑容满面地欢迎他的到来，河伯放眼望去，只见北海汪洋一片，无边无涯，他呆呆地看了一会儿，深有感触地对北海若说：俗话说，只懂得一些道理就以为谁都比不上自己，这话说的就是我呀。

Results: The renal perfusion was increased in diabetes group after the injection of alloxan. The PSI, AUC and ascending slope rate of the curve of the time-intensity curve were increased in 1w and 2w with decreased of HPT; the pathological examination exhibited glomeruli hypertrophy and no thickening of basal lamina. The PSI and AUC in 4w and 6w were more than that of control group; the PIT and ascending slope rate of the curve got closed to normal in 4w and 6w; the intercapillary cells increased. The speed of ascending slope rate of the curve in 8w, 10w and 12w was slower, shown as PIT and HPT delayed; the pathological examination showned increasing of mesangial cell, hyperplasia of mesangial matrix and thickening of basal lamina, followed with obliteration of partial micrangium, glomerular sclerosis and reduce of PSI in 12w.

结果：糖尿病组兔注射四氧嘧啶后肾皮质血流灌注增大，1周、2周表现为时间-强度曲线参数中峰值强度升高、曲线下面积增大、达峰时间缩短、曲线上升斜率升高，此时病理可见肾小球肥大，系膜基质正常，基底膜无增厚；4周、6周，峰值强度、曲线下面积高于对照组，达峰时间、曲线上升斜率接近正常，系膜细胞增多。8周、10周、12周，达峰时间延长、曲线上升斜率减慢、峰值强度减半时间延长，病理出现肾小球系膜细胞明显增多，系膜基质增生，基底膜增厚，至12周病理出现部分毛细血管管腔闭塞，肾小球节段性硬化，峰值强度测值减低。

Since the gentleman subsists at ordinary times so difficult, have got the " notorious " intensity of hesitating, on the bottom without capability, resolve in desperation, give gentleman produce one assurance can improve trick of " audience rating ", make the gentleman " more notorious ", reap fabulous profits in improving " audience rating ": Utilize one weekend, take your friend and an third-class cameraman address of reaching your mother, use the brandy or gin, make your mother drunk, deceive to filled with the open ground of the sunshine of springtiming, there are lawns, also there are flowers, in the green and shady hillside courtyard, let her take off all all sweater and pants,It lie in, sit in, stand until every one is the American and wooden to connect with because there aren't chair, take a picture by naked mother portrait of Cafferty.

既然先生平时度日如此艰难，已到不惜&臭名昭著&的程度，在下不才，索性横下一条心，给先生出一个保证可以提高&收视率&的招数，让先生更加&臭名昭著&一番，在提高&收视率&中大捞一把：利用一个周末，带上你的朋友和一位三流摄像师到你母亲的住址，用白兰地或杜松子酒，把你的母亲灌醉，骗至洒满春日阳光的开阔地，既有草坪，又有鲜花、翠荫的山坡庭院中，让她脱光了所有衣裤，卧在、坐在、立在一张美式木质连椅上均可，照一张全裸的卡弗蒂之母肖像。

Daring burglaries by armed men, and highway robberies, took place in the capital itself every night; families were publicly cautioned not to go out of town without removing their furniture to upholsterers' warehouses for security; the highwayman in the dark was a City tradesman in the light, and, being recognised and challenged by his fellow-tradesman whom he stopped in his character of 'the Captain,'gallantly shot him through the head and rode away; the mail was waylaid by seven robbers, and the guard shot three dead, and then got shot dead himself by the other four, 'in consequence of the failure of his ammunition: after which the mail was robbed in Peace; that magnificent potentate, the Lord Mayor of London, was made to stand and deliver on Turnham Green, by one highwayman, who despoiled the illustrious creature insight of all his retinue; prisoners in London gaols fought battles with their turnkeys, and the majesty of the law fired blunderbusses in among them, loaded with rounds of shot and ball; thieves snipped off diamond crosses from the necks of noble lords at Court drawing-rooms; musketeers went into St.

武装歹徒胆大包天的破门抢劫和拦路翦径在京畿重地每天晚上出现。有公开的警告发表：各家各户，凡要离城外出，务须把家具什物存入家具店的仓库，以保安全。黑暗中的强盗却是大白天的城市商人。他若是被他以&老大&的身份抢劫的同行认了出来，遭到挑战，便潇洒地射穿对方的脑袋，然后扬长而去。七个强盗抢劫邮车，被押车卫士击毙了三个，卫士自己也不免&因为弹尽援绝&被那四个强盗杀死，然后邮件便被从从容容地弄走。伦敦市的市长大人，一个神气十足的大员，在特恩安森林被一个翦径的强徒喝住，只好乖乖地站住不动。那强盗竟当着众随员的面把那个显赫人物掳了个精光。伦敦监狱的囚犯跟监狱看守大打出手；法律的最高权威对着囚犯开枪，大口径短枪枪膛里填进了一排又一排的子弹和铁砂。小偷在法庭的客厅里扯下了贵族大人脖子上的钻石十字架。

Daring burglaries by armed men, highway robberies, took place in the capital itself every night; families were publicly cautioned not to go out of town without removing their furniture to upholsterers' warehouses for security; the highwayman in the dark was a City tradesman in the light, and, being recognised challenged by his fellow-tradesman whom he stopped in his character of 'the Captain,'gallantly shot him through the head rode away; the mail was waylaid by seven robbers, the guard shot three dead, then got shot dead himself by the other four, 'in consequence of the failure of his ammunition: after which the mail was robbed in Peace; that magnificent potentate, the Lord Mayor of London, was made to stand deliver on Turnham Green, by one highwayman, who despoiled the illustrious creature insight of all his retinue; prisoners in London gaols fought battles with their turnkeys, the majesty of the law fired blunderbusses in among them, loaded with rounds of shot ball; thieves snipped off diamond crosses from the necks of noble lords at Court drawing-rooms; musketeers went into St.

武装歹徒胆大包天的破门抢劫和拦路翦径在京畿重地每天晚上出现 wWw.8tTt8.coM 。有公开的警告发表：各家各户，凡要离城外出，务须把家具什物存入家具店的仓库，以保安全。黑暗中的强盗却是大白天的城市商人。他若是被他以&老大&的身份抢劫的同行认了出来，遭到挑战，便潇洒地射穿对方的脑袋，然后8t t t8.com 扬长而去。七个强盗抢劫邮车，被押车卫士击毙了三个，卫士自己也不免&因为8 Tt t 8。 com 弹尽援绝&被那四个强盗杀死，然后8t t t8.com 邮件便被从从容容地弄走。伦敦市的市长大人，一个神气十足的大员，在特恩安森林被一个翦径的强徒喝住，只好乖乖地站住不动。那强盗竟当着众随员的面把那个显赫人物掳了个精光。伦敦监狱的囚犯跟监狱看守大打出手；法律的最高权威对着囚犯开枪，大口径短枪枪膛里填进了一排又一排的子弹和铁砂。小偷在法庭的客厅里扯下了贵族大人脖子上的钻石十字架。

After refreshing my self, I got all my Cargo on Shore, and began to examine the Particulars: The Cask of Liquor I found to be a kind of Rum, but not such as we had at the Brasils; and in a Word, not at all good; but when I came to open the Chests, I found several Things, of great use to me: For Example, I found in one, a fine Case of Bottles, of an extraordinary kind, and fill'd with Cordial Waters, fine, and very good; the Bottles held about three Pints each, and were tipp'd with Silver: I found two Pots of very good Succades, or Sweetmeats, so fastned also on top, that the Salt Water had not hurt them; and two more of the same, which the Water had spoil'd: I found some very good Shirts, which were very welcome to me; and about a dozen and half of Linnen white Handkerchiefs, and colour'd Neckcloths; the former were also very welcome, being exceeding refreshing to wipe my Face in a hot Day; besides this, when I came to the Till in the Chest, I found there three great Bags of Pieces of Eight, which held about eleven hundred Pieces in all; and in one of them, wrapt up in a Paper, six Doubloons of Gold, and some small Bars or Wedges of Gold; I suppose they might all weigh near a Pound.

我先吃了点东西，把所有的东西都搬到岸上，并仔仔细细地查看了一番。我搬回来的那桶酒是一种甘蔗酒，但与我们巴西的甘蔗酒不一样。一句话，这种酒非常难喝。可是，我打开那两只大箱子后，找到了几样东西对我非常有用。例如，在一只箱子里，有一只精致的小酒箱，里面的酒瓶也十分别致，装的是上等的提神烈性甜酒，每瓶约三品脱，瓶口上还包着银子；还有两罐上好的蜜饯，因为封口很好，咸水没有进去。另外还有两罐却已被海水泡坏了。我又找到一些很好的衬衫，这正是我求之不得的东西。还有一打半白麻纱手帕和有色的领巾。麻纱手帕我也十分需要，大热天拿来擦脸真是再爽快也没有了。此外，在箱子的钱箱里，有三大袋西班牙银币，约一千一百多枚，其中一袋里有六块西班牙金币和一些小块的金条，都包在纸里，估计约有一磅重。

I'm 14 years old and my name is Anton Hili. I now live with my four sisters and my mother. Before we lived in the mountains in a small village called Dukagjin. Ten years age, my uncle got into a fight with a neighbor. He killed him with a stone. He smashed his skull.

我今年14岁，我的名字叫安顿希里，我现在和我的四个姐姐及我的母亲生活在一起，我们原来住在山区里一个叫杜卡金的小村庄，10年之后我的叔叔和他的邻居大家，他用石头把邻居打死了，把他的骨头砸碎了。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。