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The experiment results were as following:All cytoplast recipients derived from oocytes of rabbit, bovine and goat can support early development of cloned embryo, the fusion rate of reconstructed embryos of goat-bovine and goat-rabbit were 59.91% and 74.10%, their cleaved rate were 57.48% and 72.57%, blastocyst rate were 9.59% and18.06% respectively, and the fusion of reconstructed embryos of goat-rabbit and goat-goat were 60.01% and 74.10%, their cleaved rate were 56.68% and 72.57%, blastocyst rate were 11.76% and18.06% respectively.

试验获得了如下结果:1。以山羊耳成纤维细胞为供体,以牛、羊、兔卵母细胞做为胞质受体构建重构胚,并经体外培养,发现不同的胞质受体均能支持重构胚的早期发育,但受体胞质对重构卵的重构胚早期发育速度和卵裂率均有显著影响。

Intravoneous injection of 0.5mg/kgBW jingsongling cause the 60 minutesrelaxation of goat gastric motor.The effects of jingsongling on the gastric motor ofgoat is major of effects of α2 -adrenoceptor agonist,as well as that of α1 -adrenoceptor agonist,the effects of α1 -adrenoceptor agonist concentrate at thecardic and pylorus;the function of α2 -adrenoceptor exit widely at cardic,pylorus,rumen,reticulum,omasum and abomasum,the function at different part is not the same.The lowestdose of jingsonglinginhibiting the amplitude and frequency of the gastricmyoelectric is 0.01mg/kgBW;the influence of jingsonglingto the gastricmotility of goat is dose-dependent,and the amplitude regain after the frequency ofgastric myoelectriv wave.When the goat revived,the gastric motor function is notreach the level of the normal;the major reason ofjingsongling cause the goat gastricrelaxation is the amplitude renew slowly.The intravoneous injection ofjingsonglingfirst,after 5 minutes,intravoneous injection the antagonist,the effect of prazosin and idazoxan mixed is best,which can fast reversal of theinhibition of jingsongling on the gastric motor of goat,the effects of idazoxan isgood,and the prazosin block the effection of jingsongling is better than that ofxylazine,especially at cardia and pylorus.When intravoneous injection of0.5mg/kgBW idazoxan and/or prazosin only,the amplitude of gastric myoelectric ingoat increases at a certain degree,but the frequency is not changed.

静脉注射0.5mk/kgBW的静松灵引起山羊胃蠕动弛缓长达60分钟;静脉注射0.5mg/kgBW的静松灵对山羊胃蠕动机能的影响是以α2肾上腺素受体作用为主的,兼有α1肾上腺素受体的作用,而且静松灵的α1肾上腺素受体的作用主要集中在幽门和贲门;α2肾上腺素受体的作用广泛存在于贲门、瘤胃、网胃、瓣胃、皱胃和幽门中,不同部位间有功能上的差异;静注0.01mg/kgBW的静松灵是对山羊的贲门、瘤胃、网胃、瓣胃、皱胃和幽门的肌电波频率和振幅均产生影响的最低剂量;静注一定剂量的静松灵对山羊胃蠕动的抑制作用与剂量呈相关性;而且山羊胃肌电波振幅的恢复比肌电波频率的恢复慢;苏醒时山羊的胃蠕动机能尚未恢复到正常状态;静松灵引起山羊胃弛缓的主要原因是由于胃肌电波的振幅恢复慢;静注0.5mg/kgBW的静松灵,5分钟后静注0.5mg/kgBW的拮抗剂时,咪唑克生+哌唑嗪的作用最好,能迅速地逆转静松灵对山羊胃蠕动的抑制作用,咪唑克生也有很强的拮抗作用,而哌唑嗪对静松灵的拮抗效果好于对隆朋的拮抗;单独静注0.5mg/kgBW的咪唑克生和/或哌唑嗪时,山羊的瘤胃、网胃、瓣胃、皱胃、贲门和幽门的肌电波振幅有一定程度的增强,而肌电波频率未见变化。

Three kinds of embryos' chromosome, including goat fertilization embryo, goat-goat cloned embryo and goat-rabbit cloned embryo were analyzed, The results showed that all of them have 60 chromosomes with same character, all were telocentric chromosome, besides the X-chromosome is the second larger telocentric chromosome.

经对超排获得的山羊受精胚、山羊同种克隆胚和羊-兔异种克隆胚的囊胚期胚胎染色体分析表明,三种来源的胚胎其染色体数目均为60条,且所有常染色体均为端部着丝点染色体,X染色体为第二大的端部着丝点染色体,说明山羊同种克隆胚和山羊-兔异种克隆胚均为山羊的核型,是供体细胞核在不同胞质中的重新编程的结果。4。

Theresults showed that the average gene flow was 2.0078. The genetic distance betweenTibet goat and JinTang black goat was minimum, and that between LeZhi black goat andJian Yang daer goat was maximum.

用POPGENE对7个地方山羊群体进行分析表明,群体问平均基因流为2.0078;藏山羊与金堂黑山羊群体间遗传距离最小,乐至黑山羊和简阳大耳羊群体间遗传距离最大。

The phylogeny tree indicated that Tibet goat, ChengDugoat, JinTang black goat and Beichuan white goat formed a branch, and LeZhi black goat andJianChang black goat formed a branch, and the next was Jian Yang daer goat.

以Nei's遗传距离为基础构建的系统发生树表明,藏山羊和金堂黑山羊、成都麻羊、北川白山羊聚为一类,乐至黑山羊和建昌黑山羊聚为一类,而简阳大耳羊自成一类。

In other ways of the she-goat reproductive characteristics, there are also much differences between Beichuan white goat and Chengdu grey goat or Jianchang black goat.

在繁殖特性的其它方面,与成都麻羊和建昌黑山羊也存在较大差异。

She is revealed in the Olympics special to possess mountain goat genes, effectively making her the strongest goat in Goat Village above Fit Goat.

在奥运特辑中,发现她原来也有高山山羊的基因,结果她就超越沸羊羊成为羊村最强壮的羊了。

Phylogenetic analyses revealed that there were two mtDNA lineages identified in five goat breeds including Angora goat, Shandonglaiwu black goat, Lubei white goat, Taihang goat and Wuzhumuqin goat. Population genetic structure and population expansion were carried out.

利用线粒体DNA分析安哥拉山羊、山东莱芜黑山羊、鲁北白山羊、太行山羊及乌珠穆沁绒山羊,揭示这5个山羊品种分成A和C 2个支系,并进行了群体结构和群体扩张分析。

According to the heredity diversity indexes described above of the five goat breeds, Guanzhong dairy goat had the most abundant heredity diversity and showed high polymorphism, and Xinong Saanen dairy goat and Shaannan white goat were inferior, while Angora goat and Boer goat had the lowest genetic variability.

分析结果显示,关中奶山羊的遗传多样性最丰富,表现为高度多态;其次是西农萨能奶山羊和陕南白山羊,而安哥拉山羊和波尔山羊的遗传变异程度最低。

For Xinong Saanen dairy goat, Guanzhong dairy goat, Shaannan white goat, Angora goat and Boer goat, gene heterozygosity/effective allele gene number/Shaanon information entropy /Polymorphism information content were 0.1589/1.1889/0.2955/0.1463, 0.4114/1.6981/0.6017/0.5171, 0.1653/1.1980/0.3046/0.1516, 0646/1.0691/0.1463/ 0.0625, 0.0541/1.0572/0.1270/ 0.0526, respectively.

西农萨能奶山羊、关中奶山羊、陕南白山羊、安哥拉山羊和波尔山羊的基因杂合度/有效等位基因数/Shaanon信息熵/PIC值分别为0.1589/1.1889/0.2955/0.1463, 0.4114/1.6981/0.6017/0.5171,0.1653/1.1980/0.3046/0.1516,0.0646/1.0691/0.1463/0.0625,

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