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The plasmid of pMD-T-GO and pPIC3.5K were extracted from JM109 strain and then glycolate oxidase gene fragment was cloned into the expression vector (pPIC3.5K). The recombinant plasmids were transformed into E.coli TOP 10Fˊ. The recombinant clones were identified by PCR, digested with SnaB I /Not I and then sequenced.

本文首先从大肠杆菌JM109中提取质粒pMD-T-GO和pPIC3.5K，将菠菜乙醇酸氧化酶基因c DNA片段克隆至表达载体pPIC3.5K，转化进E.coli TOP 10Fˊ，利用设计的引物进行PCR扩增、SnaB I和Not I酶切鉴定，最后进行序列分析。

Has "continued to do something " means, the difference is as follows: go on doing that continue to do, has to do something middle ...

都有"继续做某事"的意思，其区别如下：go on doing表示继续做，一直在做某事中间。。。

Police in West Palm Beach is still trying to figure out what drove a man to go on a shooting rampage at a Wendy's. The gunman opened fire, killing one and injuring another five. The man later took his own life.

West Palm Beach一家温蒂圣堡餐馆，一持枪分子（没有翻译出 go on a shooting rampage，这里可以译成"开枪扫射/滥射"）造成1人死亡，其它5人受伤，然后自杀，警察仍在试图找出该持枪分子制造屠杀（"屠杀"用在这里有点不太好）的动机。

Go on with sth.

go 和可互换。

So If I wanted to play with words, I'd say the PSP Go has already been hacked

因此如果我想要玩一个文字游戏的话，我会说PSP Go其实已经被破解了。

She wanted to quit her job but her mother told her not to go off half-cocked.

她想要辞了这工作，但她母亲说，还是不要那么仓促做决定。go off half-cocked 不成熟的，仓促的。

I swear it's... Deja... Vu Hova's flow so unusual Baby, girl you should already know It's H-O, light up the ...him That's a no-go My dough keep the scales, tippin Like 4-4's Like I'm from the H-O- U -S-T-O-N Blow, wind So ...

温暖你的脆弱只要你在就是希望从今以后都有你陪我一起走为何笑中带泪 wae u seo do nun mu ri na go 为何茶。。。geu nu gu ee mok so ri do 不管有多远多少困惑当你需要我我会在这不会躲把你的爱握在手中我的勇敢温暖你的。。。

Search engine in the initial stage of development, Li as the world's first one of the researchers, the creation of the first ESP technology, and it applies to the success of INFOSEEK / GO.

在搜索引擎发展初期，李彦宏作为全球最早研究者之一，最先创建了ESP技术，并将它成功的应用于INFOSEEK/GO。

In the early development of the search engine, as one of the earliest researchers in the world Li Yanhong created ESP technology at the earliest time and applied it successfully in the INFOSEEK/GO.

在搜索引擎发展初期，李彦宏作为全球最早研究者之一，最先创建了ESP技术，并将它成功的应用于INFOSEEK/GO。

In the search engine development initial period, one of Li Yan great achievement whole world earliest researchers, founded the ESP technology first, and application which succeeds it in INFOSEEK/GO.

在搜索引擎发展初期，李彦宏作为全球最早研究者之一，最先创建了ESP技术，并将它成功的应用于INFOSEEK/GO。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。