查询词典 go off into

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

I shut off the engine and coast into the garage. I take my shoes off before I go into the house, I sneak up the stairs, I get undressed in the bathroom.

在进屋前我脱掉鞋子，我蹑手蹑脚的上楼，我在浴室脱掉衣服，我轻手轻脚的钻进被窝。

Mosley Im glad youre my maker My Loyalty lies in your hands, youre my breath taker Your body, your kiss is in unknown demand So take command, go Timbo I be the same when it all goes up I be the same when it all goes down Not the first one, open it up I be the last one closin it out Dont know if Ill give you a shot yet Lil Mama Im peepin your style Do I think youre dope enough, yup One way of findin it out The way you came at me, boo Dont care, not afraid Im like Wild Really want it from head to toe Question if she gon let it out Anyway the hour glass go I dont worry anyhow Why dont we see where it go Lets figure it out When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after Dark Owww, Oooohhh Owww Come on SoShy I got a little secret for ya I never sleep when comes the night But everytime I smack my fingers I switch back into the light My moon belong to your sun Your fire is burning my mind Is it love or is it lust Something that I just cant describe Am I the one and only Cause youre the only one It felt so long and lonely Waiting for you to come Its lookin bright and early Im willing to close my eyes This is the unusual story Timbo and SoShy When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after When the cats come out the bats come out to playy Yeahh In the morning after The dawn is here, be gone be on your wayy Yeahh In the morning after Dark Heyy, Heyy, Heyy, Go Nelly!

莫斯利先生您好我很高兴youre进出口公司我的忠诚度掌握在你们手中，youre我的呼吸接受者你的身体，你的吻是在未知的需求因此，接受命令，去廷博俺是相同的时，所有的上涨俺是相同的时，一切都下降不是第一个，打开它俺是最后一次closin出来 Dont知道病患者给你一个镜头还进出口peepin律妈妈你的风格我认为youre涂料不够，烨一个方法是findin出来你的方式来我，嘘 Dont护理，不怕像野生进出口真的想从头到脚它问她是否坤把它放出但无论如何，沙漏去余请勿担心无论如何为什么dont我们看到它去可以让数字出来当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明，消失在您的wayy Yeahh 在上午的后当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明，消失在您的wayy Yeahh 在天黑后早晨 Owww，Oooohhh Owww 来吧SoShy 我得到了亚一个小秘密我从来不睡觉的时候来晚但是，每次我拍击我的手指我转回到光明我属于你的月亮太阳你的火是我心中燃烧是爱还是欲望一些我公正不能描述我是唯一的原因youre唯一的它认为这样漫长而孤独的等着你来它lookin一大早进出口愿意接近我的眼睛这是不寻常的故事廷博和SoShy 当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明，消失在您的wayy Yeahh 在上午的后当猫出来的蝙蝠出来playy Yeahh 在上午的后这里的黎明，消失在您的wayy Yeahh 在天黑后早晨日Heyy，日Heyy，日Heyy，直接耐莉！

So my mother gave him more good to talk about kindergarten, kindergarten and told him that every child must grow up to go over the side, he can also point to the material rewards, loaded into a small bag, he let him go to kindergarten, home can also go to the same class of children to play at home, so he naturally has a good partner willing to go.标签: cry , home , kindergarten

所以妈妈要多给他讲讲小宝宝园的好，并告诉他小宝宝园是每个小朋友成长过程中必须要去的过方，也可以给他点物质奖励，装到他的小书包里让他带到小宝宝园里去，回家后也可以把同班的小朋友请到家里来玩，这样他有了好的伙伴自然就愿意去了。

Being in London is such a great city, I met a lot of guys who worked and danced in a big club as a go-go dancer and that's where I got into a go-go dancing job and met the guy that I loved the most 'Chris Geary' he's the most sweet and loving person and been with him for 2years now.

我见到他的俱乐部，我跳舞，他和他的团伙也有舞蹈，以及到现在我们一起工作和生活中与他 London 伦敦。。

Three diagnostic criteria for diagnosing AMI were designed, namely cTnⅠ+Myo, cTnⅠ+ H-FABP, and cTnⅠ+H-FABP+Myo. Diagnostic accuracy rating of the three criteria was then calculated and compared. Results Accord- ing to the ROC curves, the cut-off values of the three biochemical markers for diagnosing AMI were defined as follows: Cut-off cTnⅠ 0.5ng/ ml, Cut-off Myo 90ng/ml, and Cut-off H-FABP 5.7ng/ml.

绘制3种标志物诊断AMI的受试者工作特征曲线并确定其最佳cutoff 值，对设计出的3种诊断AMI的心肌标志物组合试验（cTnⅠ+H-FABP、cTnⅠ+Myo、cTnⅠ+Myo+H-FABP）的诊断准确度指标进行比较。

And in that day, declares the LORD, I will cut off your horses from among you and will destroy your chariots; [11]and I will cut off the cities of your land and throw down all your strongholds; [12]and I will cut off sorceries from your hand, and you shall have no more tellers of fortunes; [13]and I will cut off your carved images and your pillars from among you, and you shall bow down no more to the work of your hands; [14]and I will root out your Asherah images from among you and destroy your cities.

并在这一天，宣告了上帝，我会切断你的马匹从你们当中，并会摧毁你的战车； [ 11 ] ，我会切断了城市的你的土地，并投掷下来你的据点； [ 12 ]我将切断sorceries从你的手，你有没有更测字的命运； [ 13 ] ，我会切断你的刻图象和你的支柱，从你们当中，你应跪拜，没有更多的工作，你手； [ 14 ] ，我会铲除你舍拉图像从你们当中，并摧毁你的城市。

The measuring device is structured by including a He-Ne laser; a beam expander, a soft aperture slot and a off-axis parabolic mirror to be measured are arranged in turn along the direction of the emergent laser beam direction of the He-Ne laser; a standard cross hair and a micro objective are arranged in turn on the direction of the reflected beam of the off-axis parabolic mirror; the micro objective is arranged on the focus of the off-axis parabolic mirror; the focus is imaged on an area array CCD; the output end of the area array CCD is connected with a computer; the off-axis parabolic mirror is fixed on a five-dimensional electric adjusting frame.

一种离轴抛物面镜点聚焦能力测定装置和测定方法，测定装置的构成包括：一台He-Ne激光器，沿该He-Ne激光出射激光束方向依次设置扩束器、软边光阑和待测的离轴抛物面镜，在该离轴抛物面镜的反射光束方向依次设置标准叉丝和显微物镜，该显微物镜位于所述的离轴抛物面镜的焦点，所述的焦点成像于面阵CCD上，该面阵CCD的输出端接计算机，所述的离轴抛物面镜固定在一五维电动调整架上。

Direct Correspondence of Display Data RAM to LCD Pixel Display Data RAM2,500 bits LCD Drivers25-common and 100-segment Selectable Duty and Bias Ratio ; 1/25 Duty 1/6 Bias or 1/15 Duty 1/5 Bias Direct connection to 8-bit Microprocessor interface for both of 68 and 80 type MPU Serial Interface (SI, SCL, A0, CS) Useful instruction set Display ON/OFF, Display Start Line Set, Page Address Set, Column Address Set, Status Read, Write Display Data, Read Display Data, Normal or Inverse ON/OFF Set, Static Drive ON/Normal Display, EVR Register Set, Read Modify Write, End, Reset, Internal Power Supply ON/OFF, Driver Output ON/OFF, Power Save and ADC select.

直接显示数据RAM的对应像素液晶显示数据RAM2，500位液晶Drivers25，共同和100段可选择的职务和偏差率； 1 / 25的责任1 / 6的偏见或1 / 15的责任1 / 5偏见的直接连接至8位微处理器的接口为68和80型微控制器的串行接口（司，沙田至中环线时，A0，CS）的相关指令集显示开/关，显示开始行设置，页面地址设置，列地址设置，状态读取，写入显示数据，读取数据显示，正常或逆开/关设置，静态驱动器/正常显示，腔内注册设置，读取修改写入，完，复位，内部电源开/关，开/关，节能和ADC选择驱动器输出。

Fall asleep, dope off, flake out, drift off, nod off, doze off, drowse off3. leave or unload, especially of passengers or cargo;同义词:drop, set down, put down, unload, discharge同义词:fall back, lose, fall behind, recede同义词:slip, drop away, fall away

本站所收集信息资料为网络转载版权属各作者并已著明作者旨在资源共享、交流、学习之用，请勿用于商业用途，本站并不保证所有信息、文本、图形、链接及其它内容的绝对准确性和完整性，故仅供访问者参照使用。

In the days of sailing vessels the crew were afraid they would be becalmed on the ocean.

在使用帆船的时代，船员们担心他们会因为无风而无法在海洋上航行。

As long as foreign donors pay the PA's salary bill, few expect a new intifada.

只要外国继续为巴权力机构的薪水买单，希望发动新暴动的人便寥寥无几。