查询词典 go and

The plasmid of pMD-T-GO and pPIC3.5K were extracted from JM109 strain and then glycolate oxidase gene fragment was cloned into the expression vector (pPIC3.5K). The recombinant plasmids were transformed into E.coli TOP 10Fˊ. The recombinant clones were identified by PCR, digested with SnaB I /Not I and then sequenced.

本文首先从大肠杆菌JM109中提取质粒pMD-T-GO和pPIC3.5K，将菠菜乙醇酸氧化酶基因c DNA片段克隆至表达载体pPIC3.5K，转化进E.coli TOP 10Fˊ，利用设计的引物进行PCR扩增、SnaB I和Not I酶切鉴定，最后进行序列分析。

They are to be stamped with Go/No-Go and size.

他们要被盖印着 'Go'和 'No Go' 和尺寸。

Twinkle twinkle can't you see need you need you but let you go somebody said to me that life is a mysiery and that you never know what's out there awaiting for you everything is meant to happen, to write a story of life even when you are so alone even when you are so afraid with this loneliness you'll be alright 'cuz it will lead your way come on twinkle why don't you clap your hands just open your door for the belter tomorrow and play your own life like you're a movie star don't you know that we're all dreamers twinkle twinkle can't you see need you need but let you go the future's in your hands and heres the map showing the way to find yourself again, a part of you we never knew tell yourself it'll be alright to give up and swallow your pride believe in yourself don't be afraid all your worries will go away because you will fight to win your life with strength to carry on come on twinkle why don't you clap your hands just open your door for the better tomorrow and play your own life like you're a movie star don't you know that we're all dreamers suddenly the sadness took my soul away like a rain on perfect day but let me go and live my life come on twinkle why don't you clap your hands play your own life like you're a movie star come on twinkle why don't you clap your hands just open your door for the better tomorrow and play your own life like you're a movie star don't you know that we're all dreamers and we walk out the door.

闪耀闪耀你不能看见需要你需要你但是让你去有人向我说生活是一mysiery 和你从不知道所的什么无法使用那里为了你等待每件事被意味着发生，写生活的一个故事甚至当你是如此孤独的甚至当你这孤独的如此害怕你将是alright'cuz它将领导你的方式在上来闪耀为什么你不拍击你的手公正敞开你的地带的门明天和玩你像你一样的自己生活是一个电影明星你不要知道我们都是梦想者闪耀闪耀你不能看见需要你需要但是让你去未来你的手同时，继承人显示方式的地图再一次为了找到你自己，一部分你我们从不知道告诉你自己它将是alright 为了放弃并且吞咽你的骄傲相信你自己不害怕所有你的担心将离开因为你将战斗赢得你的生活具有力量继续在上来闪耀为什么你不拍击你的手公正敞开你的门更好明天和玩你像你一样的自己生活是一个电影明星你不要知道我们都是梦想者悲哀突然地拿我的灵魂像一场雨一样完美天但是，让我去并且过着我的生活在上来闪耀为什么你不拍击你的手玩你像你一样的自己生活是一个电影明星在上来闪耀为什么你不拍击你的手公正敞开你的门更好明天和玩你像你一样的自己生活是一个电影明星你不要知道我们都是梦想者同时，我们出去door。

The Go moved backward with 1.35mm and upward with 1.90mm in group I, the gonial angle increased for 3.69°, and angle between palatal plane and mandibular plane was increased for 5.00°; In group II, the Go moved backward with 1.85mm and upward with 3.32mm , the gonial angle increased for 4.06°, and angle between palatal plane and mandibular plane was increased for 5.22°. Both in group I and II, there was a tread for clockwise rotation of mandibular corpus. The length of pterygomasseteric sling was mild increased due to the backward movement of Go.

在Go点的变动方面，在Me点向前位移的族群里，术后Go点平均向后1.35mm，向上1.90mm， gonial angle 平均增大3.69度，腭平面与下颚平面的夹角则是增加5.00度；在Me点向后位移的族群里，术后Go点平均向后1.85mm，向上3.32mm， gonial angle 平均增大4.06度，腭平面与下颚平面的夹角则是增加5.22度，不论是向前或是向后的族群，结果皆显示翼咀嚼肌悬吊带有轻微被拉长的迹象。

Purified GO had a molecular weight between 145, 000 and 162, 300, as determined by Sephadex G-200 filtration and SDS-PAGE of the enzyme as cross-linked with glutaraldehyde. This result showed that the holoenzyme was a tetramer, and no change occurred in molecular weight of the enzyme during the s〓orage. The active form of GO from brassica leaves showed a constant molecular weight during purification, so did that from O. sativa leaves during greening of etiolated seedlings, maybe acting as a stable tetramer.

用Sephadex G-200胶过滤和戊二醛交联后的SDS-PAGE法测得纯化的菜心叶片GO的分子量在145，000-162，300间，为四聚体；并且在放置过程中其分子量和聚合态均保持不变，为稳定的四聚体；进一步研究表明菜心叶片GO在纯化过程中和水稻叶片GO在黄化苗的转绿过程中，两种GO活性状态的分子量和聚合态也没有发生变化，均呈四聚体。

Based on the analysis of lichee cold-chains, this paper builds up a GO-FLOW chart for the systems, discusses the signals, operators and calculation rules. GO algorithm is performed to determine the reliability and failure probability of every network nodes, so as to find out the key nodes that affect the safety of lichee logistics systems, ways to improve it.

在对荔枝冷链物流系统进行分析的基础上，建立荔枝物流系统的GO-FLOW图，并分析了其信号流、操作符和运算规则；以具体荔枝物流过程为例，进行GO运算，求出各信号流的故障率和可靠性，找出影响物流安全的关键环节和提出解决方法；指出GO-FLOW法是物流安全评价的一个有效和方便的方法。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

One Wild Night It's a hot night, the natives are restless We're sweating by the light of the moon There's a voodoo mojo brewing at the go-go That could knock a witch off her broom We slither on in and shed our skin Make our way into the bump and the grind So I'm passing by, she gives me the eye So I stop to give her a light Chorus: One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone And she left my heart with vertigo) One wild, one wild, one wild, one wild night Havin' as much fun as you can in your clothes Margarita had me feelin' alright It just might be that I found religion I've been on my knees for half of the night Then I'm rolling the bones with Jimmy 'no dice' Gonna take him for a couple weeks' pay Man, if you lose this roll I take your girlfriend home So I stopped But you're not gonna believe who comes walking out Chorus: One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone And she left my heart with vertigo) One wild night (hey, c'est la vie) One wild night One wild night (life is for the living so You gotta live it up, come on let's go) One wild, one wild, one wild, one wild night Guitar solo Chorus: One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone And she left my heart with vertigo) One wild night (hey, c'est la vie) One wild night One wild night (life if for the living so You gotta live it up, come on let's go) One wild, one wild, one wild, one.. One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone) For one wild night One wild night (hey,c'est la vie) One wild night One wild night All we've got is one wild night One wild night (4x) One wild night blinded by the moonlight One wild night 24 hours of midnight One wild night stepped into the twilight zone for One wild, one wild, one wild, one wild night.

一狂放的夜它是热的夜，当地人是不安定的由那里月亮的光冒汗是巫毒教mojo酿造在可能敲巫婆她的笤帚的去去的我们我们滑行并且流洒我们的皮肤做我们的方式成爆沸和研磨，因此我通过，她给我眼睛，因此我停下来给她轻的合唱：一狂放的夜一狂放的夜(24个小时午夜)一狂放的夜(我跨步入暮色区域和她左我的心脏以眩晕)一狂放，一狂放，一狂放，一狂放的夜 Havin同样多乐趣，象您在您的衣裳玛格丽塔酒能有我feelin它也许行是我发现了我是在我的膝盖为夜的一半的宗教我然后滚动骨头与吉米'没有模子' 去采取他为几星期的薪水人，如果您丢失我采取您的女朋友家的这卷，因此我停止了，但您不相信谁来走出去 Chorus： One wild night One wild night (24 hours of midnight) One wild night (I stepped into the twilight zone And she left my heart with vertigo) One wild night (hey， c'est la vie) One wild night One wild night (life is for the living so You gotta live it up， come on let's go) One wild， one wild， one wild， one wild night Guitar solo Chorus：一狂放的夜一狂放的夜(24个小时午夜)一狂放的夜(我跨步入暮色区域和她左我的心脏以眩晕)一狂放的夜(嘿， c'est la竞争)一狂放的夜一狂放的夜(生活，如果为生活，因此您得到居住它，进展我们是)一狂放，一个狂放，一个狂放，一个一狂放的夜一狂放的夜(24个小时午夜)一狂放的夜为一狂放夜一个狂放的夜(嘿， c'est la竞争)一狂放的夜一狂放的夜我们有的全部是一狂放的夜一狂放的夜(4x)一狂放的夜由月光蒙蔽了一狂放的夜24个小时午夜狂放的夜跨步入暮色区域为一狂放的一，一狂放，一狂放，一狂放的夜。

Karen's Gaelic singing is well-represented, notably on the lovely Seinneam Cliù Nam Fear r, the vocal highlight of the album. She switches to English for a brace of anti-war songs, John Martyn's poignant Don't You Go and Donald Shaw's Soldier Boy.

Karen的声音也依旧动人，温柔里的魄力在两首反战歌曲Don't You Go和Soldier Boy里表现得淋漓尽致。

Exerchomai {ex-er'-khom-ahee} from 1537 and 2064; TDNT - 2:678,257; v AV - go out 60, come 34, depart 28, go 25, go forth 25, come out 23, come forth 9, misc 18; 222 1 to go or come forth of 1a with mention of the place out of which one goes, or the point from which he departs 1a1 of those who leave a place of their own accord 1a2 of those who are expelled or cast out 2 metaph. 2a to go out of an assembly, i.e.

相关经文回前一页 1831 exerchomai {ex-er'-khom-ahee}源自1537和2064； TDNT - 2：678，257；动词 AV - go out 60， come 34， de分词28， go 25， go forth 25， come out 23， come forth 9， misc 18； 222 1 出来，出去 1a 伴随著一个人提及要去的地方，或者离开的所在 1a1 一个人自动地离开一个地方 1a2 一个人被赶出去，或者被逐出去 2 隐喻。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。