查询词典 glycerophosphate

MSCs were cultured in a conditional medium in subculture including: dexamethasone, vitamin C, and β-glycerophosphate. At the same time, we constructed the eukaryotic expression vector containing VEGF165gene. VEGF165 gene was transfected into MSCs by means of LipofectAMINE?2000. The stably gene expressive cells were screened with G-418 for 14 days.

在脂质体介导下将构建成功的真核表达载体pcDNA3.1- VEGF165转染到诱导培养的MSCs，G-418筛选阳性细胞克隆，将阳性克隆细胞和未转染的MSCs共同接种于β-磷酸三钙上，体外培养7天后，植入原大鼠肌肉内。

Their dynamic morphological changes,growth curve,glycerophosphate dehydrogenase pattern,oil red O staining,and reaction to insulin and dexamethasone all verified their preadipocyte identity.

经形态学动态变化、生长曲线、甘油磷酸脱氢酶活性变化、油红O脂肪染色及对胰岛素和地塞米松反应的测定，证明是功能活跃的前脂肪细胞，并在体外重现了其增殖、肥大的全过程。

The passage 3 rat bone marrow mesenchymal stem cells were cultured at the density of 5×107/L in different groups, the induced group was added into conditioned medium containing 1×10-8 mol/L dexamethasone, 10 mmol/L beta-sodium glycerophosphate and 50 mg/L ascorbic acid at the same time of seeding, at 50%-60% confluence, at 85%-90% confluence, respectively.

将第3代大鼠骨髓间充质干细胞以5×107 L-1密度分组培养，诱导组分别于接种当时、细胞达50%~60%融合时、细胞达85%~95%融合时加入含地塞米松、β-甘油磷酸钠、维生素C的成骨诱导培养基诱导。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂（β-甘油磷酸钠，地塞米松，维生素C）、成脂肪细胞诱导液（地塞米松，甲基异丁酸黄嘌呤，牛胰岛素，吲哚美辛）及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化，经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物（碱性磷酸酶、骨钙素mRNA、钙结节）、脂肪标志物（脂滴、PPARγ-2mRNA）、以及类神经标志物（尼克氏体、神经烯醇化酶、神经丝蛋白）。

The results showed that, after induced for 4~5 d by dexamethasone, beta-glycerophosphate and ascorbate-2 phosphate, the volume of some cells of the goat MSCs gradually largened and changed their form from spindle/polygon to cuboid/ovoid; With the induce time continuing, the numbers of the cuboid/ovoid cells continuous increased; At 15th day, there were many cuboid cells, and the modality of the cells changed from cuboid to ovoid; At 25th day, the oval and cuboid cells accumulation were observed.

结果表明，山羊MSCs在地塞米松、β-磷酸甘油和抗坏血酸的作用下，从诱导的第4~5天开始，即可见有些MSCs体积逐渐增大，由梭形或多角形转变为立方形或卵圆形；随着诱导时间延长，立方形或卵圆形细胞不断增多，到第15天时，有较多的立方形细胞，且这种变化经历了从立方形向卵圆形转变的过程；第25天，可见卵圆形和方形细胞堆积，诱导分化率为90.2%± 2.97%。

METHODS: The subcutaneous adipose tissue was obtained from adult New Zealand rabbits under aseptic condition, and cultured in vitro with collagenase digestion. All cells were divided into 3 groups: in the BMP-2 group, cells were cultured with medium containing 0.1 g/L vitamin C, 10 mmol/Lβ-sodium glycerophosphate and 10μg/L BMP-2 for 10 minutes, followed by 4-14 days inoculation with density of 18×104 cells per pore. In the BMP-7 group, cells were cultured with BMP-7 with the same methods as BMP-2 group. The cells were cultured with simple culture medium in the control group.

无菌切取成年新西兰大白兔皮下脂肪，胶原酶消化法体外分离培养脂肪干细胞，分为3组：骨形成蛋白2组加入含0.1 g/L维生素C、10 mmol/L β-甘油磷酸钠、10 μg/L骨形成蛋白2的诱导培养基培育15 min，然后按18×104个细胞/孔接种，再培育4~14 d；骨形成蛋白7组培养方法基本相同，仅将骨形成蛋白2更换为骨形成蛋白7；对照组同法加入单纯培养基进行培育。

The classic osteoblast-induced system including 0.1μmol/L dexamethasone, 10 mmol/Lβ-glycerophosphate, 50μg/mL Vitamin C was used to induce osteoblastic differentiation of BMSCs. Through the detection of alizarin red staining and quantitative, the ALP activity and the expression of OCN-mRNA, it explored the effect of osteoblastic differentiation of BMSCs on Bushen Therapy, Huatan Therapy, Bushen Huatan Therapy.

使用0.1μmol/L地塞米松，10 mmol/Lβ-甘油磷酸钠，50μg/mL Vitamin C联合诱导BMSCs成骨分化，通过检测茜素红染色及定量、碱性磷酸酶活性和骨钙素mRNA表达，探讨补肾中药、化痰中药和补肾化痰中药对BMSCs成骨分化的影响。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷