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The stressed expression of Bmhsp19.9 could be divided into three typical patterns among which the intensive response expression of testis, posterior silk gland and fat body was the main one.

其应激表达模式可分为3类,以其在精巢、后部丝腺和脂肪体中的强应激表达模式为主,经热刺激处理后其表达量有显著增加。

RT-PCR detection and analysis of gene chip data showed that silkworm mago nashi gene expressed in all the stages detected during silkworm development, including embryo, larva, pupa and moth. However, higher expression could be visualized in gonads and silk gland compaired with other tissues.

RT—PCR检测和组织芯片数据分析结果表明,家蚕mago nashi基因在胚胎、幼虫、蛹和蛾期都有表达,在5龄第3天幼虫的各组织中也均有表达,但在生殖腺和丝腺中的表达量高于其它组织。

Silk gland cell, by the Grace nutrient fluid in vitro culture, then after 150d for primary culture, passage was taken, later each approximately 15d take a passage, after that, the time of passage cut to 10 day.

经210d左右的原代培养后进行传代培养,以后每隔约15d传代一次,后传代时间缩短到10天,当传代时间稳定在7天时细胞开始趋于稳定,此时鉴定细胞系的生物学特性。

It was found that the silk fibroin obtaineddirectly from anterior and middle regions of middle division of silk gland is in orderedstate, assumed to be in nematic form, but not observed in posterior region, in whichliquid silk fibroin is in coiled coil and some α-helix forms. In the middle region of middle division there is a relatively regular arrangement for the α-helix, and there is arelatively regular arrangement for β-strand and loosed α-helix in anterior region.

在熟蚕丝腺体的中部前、中段的丝心蛋白处于类向列型液晶的有序态,而中部后段中则观察不到此有序态,而是呈无规线团和一些α-螺旋结构,而且中部前中段的结构也有区别,中部中段的有序态为α-螺旋结构的相对规则聚集,而中部前段的则为β-伸展链和松散α-螺旋结构的相对规则聚集。

The contents of metal elements in silk gland and silk fiber of B . mori silkworm have been detailedly analyzed by proton induced X-ray emission, inductively coupled plasma mass spectroscopy and atomic adsorption spectroscopy.

用不同的测试方法,即质子诱导X射线发射、电感耦合等离子体质谱和原子吸收光谱对桑蚕丝腺体和丝纤维中金属元素的含量进行了详细的表征。

In this paper, the fib-H promoter was choned by PCR method and sequenced. Sequence analysis showed that the cloned fib-H promoter harbored the typical motifs, which resulted in the gene expressing in the silk gland specifically.

为利用其时空调控机制,通过PCR扩增方法克隆fib-H启动子片段,并进行序列分析,进而构建由fib-H启动子控制报告基因DsRed的重组载体pSK-FH-DsRed-PolyA,通过家蚕BmN细胞和丝腺进行瞬时表达。

There is a mature process with silk fibroin descending down the gland and the structure of silk fibroin changes gradually, and forms a high viscous gel with some oriented structure finally.

随着丝素蛋白水溶液在腺体内的前移,丝素蛋白分子逐渐沿移动方向取向而呈有序态,最终成为粘度非常大的各向异性的凝胶体,而再生丝素蛋白水溶液是粘度非常低的各向同性溶液。

The results showed that Na, Mg, K, Ca, Cu, Zn, Fe and Mn were found in both silk gland and silk fiber, while Rb and Sr were also possibly contained.

结果表明,在桑蚕丝腺体和丝纤维中含有钠、镁、钾、钙、铜、锌、铁、锰八种金属元素,同时还可能含有微量的铷和锶。

The contents of metal elements in silk gland and silk fiber of B. mori silkworm have been detailedly analyzed by proton induced X-ray emission, inductively coupled plasma mass spectroscopy and atomic adsorption spectroscopy.

摘要用不同的测试方法,即质子诱导X射线发射、电感耦合等离子体质谱和原子吸收光谱对桑蚕丝腺体和丝纤维中金属元素的含量进行了详细的表征。

The effects of feeding alanine-enriched mulberry leaves on the larval weight, silk gland weight and cocoon characters of the silkworm Bombyx mori L. were studied.

本研究以添加丙氨酸的桑餵食桑蚕Bombyx mori L。,解其对桑蚕幼虫重、丝腺重、蚕茧特性所造成的影响。

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