查询词典 ghost effect

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

His first major work as rapper/producer was for Cantopop star Eason Chan. In 2001, Ghost Style was invited to remix one of Chan's songs for a remix project Mixed Up. At the same time Ghost Style met international dance music producer Dan F. At that time Dan F was creating breakbeat tracks and both collaborated on the remix. Dan F who won two Breakbeat Awards in the UK, would be Ghost Style's collaborator on 2002 release Alias and Ghost Style's 2005 release Message Is Complete. Ghost Style was the voice talent in the 2005 movie soundtrack release Initial D.

他接手的第一份大case就是担任陈奕迅的说唱和音及制作人2001年，Ghost Style被邀请作陈奕迅的混音及后期制作正是此时，Ghost Style遇到了国际舞曲制作人Dan F，那时Dan F也在负责制作这套混音的节拍与音轨——Dan F曾在英国拿过两个专业的节拍制作奖Ghost Style分别在2002年《Alias》和2005年发行的《Message Is Complete》两张专辑中，Dan F也与他一起合作2005年的电影《头文字D》中，Ghost Style充分展现出了他天生的音乐才华

Man hollowing out a pumpkin is also engraved with a face point, the ghost of the candle used to disperse; Another point is the ghost of the candle, trying to cheat the people taken in and follow the ghost to go, so people on the surface of the pumpkin engraved with a mockery of the face to make fun of the ghost: Humph!

一种说是人挖空了南瓜又刻上鬼脸点上烛火用以驱散鬼魂的；另一种说是鬼魂点上的烛火，试图骗取人们上当而跟着鬼魂走，所以人们就在南瓜表面刻上一个嘲讽的脸面，用以调笑鬼魂：哼！

Of the ghost novels in Wei-Jin Dynasties, stories about ghost and people in Tang Dynasty, the novels of script for story-telling in Song Dynasty; the mystery desalinize gradually, the air of the common customs is strengthened gradually. The ghosts appearance is to serve reality purport of the works, to solve the realistic contradiction, not merely to tell such odd and unusual ghost stories.

魏晋志怪中的鬼话，唐代人鬼故事，宋代话本小说，其中的鬼怪谲异成分逐渐淡化，人间世俗之气逐渐增强，鬼魂的出现是为了解决现实矛盾，实现作品主旨，而不仅仅在讲述一个个离奇怪异的鬼故事。

Second, when the ghosts have to know and learn the skills LG is ajump, you have to kill him in the police when a large role, and some ghost and I said the first step in profiling, profilingwas killed on the possibility of a big, in fact, a ghost from the jump start It was not until that you want to go to thelocation of profiling can not, so you do not have a ghost dance step.

其次，当鬼必须知道并学会的技巧是LG跳，他在你杀警的时候作用很大，有的鬼说我第一步显形，显形被杀的可能性就很大了，其实鬼跳从开始到一直到你，想要去的位置可以不显形，所以是你没有掌握鬼跳第一步。

The vast majority of people are jumping the so-called "ghost dance", as Lee jumped fruit at a fairly rapid pace, CT and running almost the speed, I believe the "ghost dance" ghost has been such a jump: what is In the future to jump out far, far away and who want to jump to escape, careful experience experience,"Lee jumped fruit," the law of the button, if Liancheng, including vertical and horizontal battlefield you!!

绝大多数的人的跳都是所谓的"鬼跳"，因为李果跳的速度相当快，和CT跑步的速度差不多，相信所有会"鬼跳"的鬼都有过这样的跳：就是一下往后跳出去好远好远，再想跳就跳不出来了，仔细体会体会"李果跳"的按键规律，如果能练成，包你纵横沙场！！

Yet it must be noted here the ghost in a straight line should not jump (out of 3, except on behalf of),Look at the radar and found that the hall also has teammates in the ghost dance, to make full use of ghost dance features consistent jump around a circular motion as long as there are latent with a 3-4, issued by the sound of breathing can be certain of successInterfere with the opponent, this time as long as you defend the scope of Flying, we must not hesitate, he will be killed (the two stand together to defend the case should make a prompt decision).

但要注意的是此地切忌直线的鬼跳（跳出3代除外），多看看雷达，发现大厅中也有队友在鬼跳的话，尽量利用鬼跳连贯的特性左右转圈跳，只要有3-4个潜伏者在一起，发出的呼吸声一定可以成功的干扰到对手，此时只要保卫在你的飞刀范围之内，一定要毫不犹豫地将他砍杀（两个保卫站在一起的话更要当机立断）。

Then the teacher pointed out that "a ghost", a "ghost" of the word imperialism is not only reveals the nature of blood-sucking demons, but also to enable students to produce such a "ghost" of the idea of a strong interest on the new courses to enhance intellectual curiosity.

接着老师指出"是鬼"，一个"鬼"字，既揭示了帝国主义是吸血的魔鬼的本质，又使学生产生为何是"鬼"的想法，对新课产生浓厚的兴趣，增强求知欲。

X are under implementation, there is a need for hard drive cloning, please enter the Ghost subdirectory, run Ghost.exe procedures should be noted that, if it is in The program runs under DOS when running the program to start before the best mouse driver for DOS, because the operation of Ghost is like a window screen frame, use the mouse to click to choose will be more convenient - although it can be used to operate the keyboard.

Ghost就是克隆硬盘的程序，该程序在DOS下、Windows9.X下都可执行，所以要进行硬盘的克隆，请先进入到Ghost子目录，运行Ghost.exe程序，需要注意的是，如果是在DOS下运行该程序时，在运行该程序前最好启动DOS的鼠标驱动程序，因为Ghost的操作画面是仿窗口画面，使用鼠标点击来选择会方便一些--虽然也可以用键盘来操作。

Ghost Gu Xiuyi it is the millet that express spirit live in seclusion inside the ghost cereal hole on bluff of day door hill, study The Book of Changes, impart the result of ghost cereal god that local common people improves the health, namely the big commonplace hard qigong that renown Wen Tian issues now.

鬼谷修易是传鬼谷子在天门山绝壁上的鬼谷洞内隐居，研习易经，并传授当地百姓强身健体的鬼谷神功，也就是现在名闻天下的大庸硬气功。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。