查询词典 get-together

Results In 60 cases, both oviduct get through in 42 cases , account for 70%;one oviduct incompletely get through in 6 cases, account for 10%;both oviduct were blocked in 12 cases, account for 20%.

结果：60例中，双侧通畅者42例，占70%；一侧通而不畅者6例，占10%；双侧输卵管不通者12例，占20%。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

People always get a little panicky when there's talk of shortage, because we all want what we can't get.

当谈到商品紧缺时，人们总是有点慌，因为我们都想买到我们所要的商品。

PARR: You can make up some of the shortfall in 15 years, but you'll not get back to the pension that you thought you were going to get.

帕尔：15年之内，你可以弥补一部分损失，但你却拿不回你认为自己应该得到的那部分养老金了。

Therefore,it is necessary to research diffusion equation for suspended sediment because it describes the sediment move process in the water body.The equation is a various coefficients second-order linear partial differential equa-tion,such equation under complex boundary condition is very difficult to get its analytical solution,while its numerical solution relative analytical solution is more easier and has the obvious superiority:simple,the computation convenience.but to get a kind of difference format which is good accuracy and stability is not easy.

泥沙扩散方程实际上是一个变系数的二阶线性偏微分方程，这样的方程在各种复杂边界条件下求解是十分困难的，求它的解析解在数学上存在着难以克服的障碍，无法求出其精确解，因此常用数值方法求它的近似解，相比较而言，数值方法有着明显的优势：即简单灵活、计算方便快捷，但要寻找一种精度高、稳定性好、计算方便的差分格式也并非易事。

The difficulties that the beginning physics teachers meet in the process of their professional growth mainly includes: firstly, pedagogically speaking, how to accomplish the transformation from textbook knowledge to practical operation, from indirect experience to direct experience; how to get practiced in the course-book and acquainted with the students, how to design the teaching plan and the classroom teaching, how to induce students to get engaged in scientific probe, to develop effectively the physics curriculum resource and to give vivid and examples.

初任物理教师在专业成长过程中所遇到的困难主要有：在教学方面，如何实现从书本知识到实际操作、从间接经验到直接经验的转化，如何熟悉教材、熟悉学生，怎样进行教学设计、课堂教学，如何引导学生进行科学探究、有效开发物理课程资源、举出形象直观生动的实例等；在班级管理方面，如何有效经营班级、评价学生等；在人际交往方面，如何与学校相关职能部门疏通好关系、如何与学校相关人员处好关系、如何与学生家长进行有效沟通交流等。

All we know is that Percy had to get top grade N.E.W.Ts to get into the ministry.

所有我们知道的，就是珀西为了进入魔法部必须在N.E.W.T考试中得到最高级别。

Would be expected to perform experiments to get to root cause when production issues and get solutions.

当有生产问题时，要能做实验，找出根本原因并提供解决方案

Eg. In order to get a job, he has to get a PhD.

你要做的就是遵守这些规则。

Well, if you wanna get philosophical, I'll get philosophical with you.

好吧，如果你想一本正经我就对你一本正经

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐