查询词典 get into lines

Special line: PET bottles to send the wind line, PET bottles transmission line, eight-treasure Li transportation, storage assembly line speed chain, refrigerator-line detection of, stainless steel transmission lines around with CPT, conveyor Store line, groove belt, transportation of finished Line, the size and quality control systems, improve water vat, with tailgate climbing belt, with the assembly line stands, the knife-edge transition belt turn, to roll lines, cell delivery systems, computer assembly lines, power assembly lines, power lines Raceway , To send the wind-line, high-speed sorting lines, transmission lines Raceway, drum line, after the assembly lines overhang, the chemical industry transmission lines, aging transmission lines, Tetra transmission lines, transmission lines plate chain, chain lost keel box line, spiral Chain plate line, spiral belt, a motorcycle transmission lines, motorcycle assembly line, climbing belt, a belt transmission line group, bottles of beer delivery systems, aerodynamic balance suspended, the lines between the pneumatic hoist, automotive transmission lines, light-top Transfer or devices, thermal plastic packet transmission line, stretching line wheel, food delivery lines, transmission lines, transmission lines climbing boxes, fruit cleansing transmission lines, the level of circulating conveyor chain, no power lines Raceway, no power telescopic Raceway lines, lines between the hoist, the letter-box sorting systems, conveyor suspension, tobacco conveyor, the parcel sorting conveyor systems, packet transmission lines, packet transmission line combinations, straight plate section, gravity-shelf access device , Heavy-duty power lines Raceway, heavy-duty conveyor chain, chain of beads to lose face envelope, turning plate, vehicle loading and unloading conveyor, the assembly line, assembly line, automatic transmission storage systems, feeding

特殊流水线：PET瓶风送线，PET瓶输送线，八宝粥输送骊，倍速链储装配线，冰箱检测线，不锈钢输送线，彩管绕带机，仓储输送线，槽型皮带机，成品输送线，尺寸与质量检测系统，大桶水提升设备，带挡板爬坡皮带机，带灯架装配线，刀口过渡转弯皮带机，地滚线，电池输送系统，电脑装配线，动力装配线，动力滚道线，风送线，高速分拣线，滚道线输送系统，滚筒线，后悬装配线，化工行业输送线，老化输送线，利乐包输送线，链板输送线，龙骨链输箱线，螺旋链板线，螺旋皮带机，摩托车输送线，摩托车装配线，爬坡皮带机，皮带输送线组，啤酒瓶输送系统，气动平衡吊，气动线间提升机，汽车输送线，轻型顶升移载装置，热塑膜包输送线，伸缩滚轮线，食品输送线，输送线，输箱爬坡线，水果输送清洗线，水平循环链式输送机，无动力滚道线，无动力伸缩滚道线，线间提升机，信盒分拣系统，悬挂输送机，烟草输送机，邮包输送分拣系统，邮包输送线，邮包组合输送线，直段板式机，重力式货架存取装置，重型动力滚道线，重型链式输送机，珠面链输包线，转弯板式机，装卸车输送机，装配流水线，装配线，自动仓库输送系统，上料机

Of 12 tomato lines, the analysis on the 4 fruit nourishment quality traits (the contents of vitamin C, total soluble solids and dry matter and ratio of sugar-acid ) was carried out to study the four kinds of disparities, which included the interspecific one, the one between two varieties in the same species , the one between the lines bred by different means in the same variety ,and the one between the different fruit-shaped lines in the same variety .The analytic method of orthogonal comparison of single degree of freedom was applied. The results showed that the line of Lycopersicum pimpinellifolium was better than the average of the lines of L.esculentum in the 4 traits at significant level of 1%, the average of the lines of var.cerasiforme was better than the average of the lines of var.vulgare in the 4 traits at significant level of 5% or 1%,and the two space mutation line(YH02-2 or YH02-6) was better than the average of the other lines of the same variation in the 4 trait, respectively, at significant level of 1%. Of the lines of var.cerasiforme , the lines with pear fruit were lower than the average of the lines with round-shaped or plum-shaped fruits in the 2traits(the contents of vitamin C and total soluble solids), at significant level of 1%,the lines with plum-shaped fruits were higher than the average of the lines with round-shaped fruits, at significant level of 1%,and the lines with yellow-colored fruits(YH02-11) was lower than the average of the lines with red-colored fruits(YH02-1and YH02-7) in the trait of the contents of vitamin C.

对12个品系进行种间、种内变种间、变种内不同选育方式间、变种内不同果实形状间在果实营养品质（果实维生素C含量、可溶性固形物含量、干物质含量和糖酸比）上的差异进行分析，采用统计学上单一自由度正交比较分析法与植物学分类相结合的方法进行分析，结果表明：醋栗番茄种品系YH02-12在4个营养品质性状上，极显著高于普通番茄种品系；樱桃番茄变种品系在4个营养品质性状上的平均水平极显著或显著高于普通番茄变种品系的平均水平；2个空间诱变品系YH02-2、YH02-6在4个营养品质性状上均极显著高于同变种的其他品系的平均水平；樱桃番茄变种品系中，梨形果品系YH02-5、YH02-8的果实维生素C、可溶性固形物含量均极显著地低于樱桃番茄变种中圆形或李形品系的平均水平，李形果品系YH02-9在4个营养品质性状上均极显著地高于圆形果品系的平均水平，黄色圆形果品系YH02-11的果实维生素C偏低于红色圆形果品系YH02-1和YH02-7。

Current various product lines are as follows: 3 static press Molding Line, 5 Air Impact lines, 6 Resin Sand lines, 2 Lipid Vulcanize Water Glass Sand lines, 10 Jolt-Squeeze Mechanical Molding lines, 3 Shell Molding lines with Resin Sand, 4 Sand Coated Iron Mould lines used for Ferrous Moulding, 11 investment casting lines, 5 Precision Casting of Lost Form lines, 27 Die Casting lines, 22 Gravity Casting of non-ferrous Metal lines and 16 Low Pressure Die Casting lines.

现有各种生产线构成如下：静压造型线3条，气冲生产线5条，树脂砂生产线6条，脂硬化水玻璃砂生产线2条，震压式机械造型生产线10条，壳型铸造生产线3条，黑色金属铁模覆砂生产线4条，熔模精密铸造生产线11条，消失模生产线5条，压铸生产线27条，有色金属重力铸造生产线22条，低压铸造生产线16条。

Static press Molding Line, 5 Air Impact lines, 6 Resin Sand lines, 2 Lipid Vulcanize Water Glass Sand lines, 10 Jolt-Squeeze Mechanical Molding lines, 3 Shell Molding lines with Resin Sand, 4 Sand Coated Iron Mould lines used for Ferrous Moulding, 11 investment casting lines, 5 Precision Casting of Lost Form lines, 27 Die Casting lines, 22 Gravity Casting of non-ferrous Metal lines and 16 Low Pressure Die Casting lines.

现有各种生产线构成如下：静压造型线3条，气冲生产线5条，树脂砂生产线6条，脂硬化水玻璃砂生产线2条，震压式机械造型生产线10条，壳型铸造生产线3条，黑色金属铁模覆砂生产线4条，熔模精密铸造生产线11条，消失模生产线5条，压铸生产线27条，有色金属重力铸造生产线22条，低压铸造生产线16条。

The company's major machines and equipment are imported from abroad, equipped with many H-type steel assembing correction lines, CNC flame-cutting production lines, gantry submerged arc welding production lines, sand-blowing rust-removing lines, painting lines, space-frame production lines,C/Z-type production lines, all kinds of plate production lines and so on.

公司的部分主要设备均从国外进口；配置多条H型钢组立校正生产线、CNC电脑数控火焰切割生产线、门型埋弧焊接生产线、喷沙除锈生产线、喷漆生产线、网架生产线、C/Z型钢生产线、各种板材生产线等先进的机器设备，使产品有了高品质的可靠保证。

Disclosed is a leaky-wave dual polarized slot type antenna, including: first and second feeding circuit sections comprised of N-first strip lines and N-second strip lines with a loop every first period along the X-axis on the first dielectric layer and a second period along the Y-axis, in which the N-first strip lines and the N-second strip lines are parallel to each other being alternate, and each length of Ls1 and Ls2 for the first period satisfies the equation of , first and second multi-channel dividers formed at once and the other sides of the first dielectric layer, to connect the N-first strip lines and the N-second strip lines parallel with each other; and first and second central ports formed in the opposite direction of the cavity, each of the feeding circuit sections being connected to the first and second multi-channel dividers; and first and second slot sections being formed by patterning the second shielding layer, in which M-first and M-second slots are arrayed along the direction of the X-axis and each of the first and second slots forms N-row first and N-row second slot arrays, respectively, which cross the first and second strip lines for each, the first slot and the second slot being orthogonal to each other.

公开了一种漏泄波双偏振槽型天线，包括：第一和第二馈电回路部分，其具有沿着X轴在第一介电层每第一周期以及沿着Y轴的第二周期形成具有环路的N第一带状线和N第二带状线，其中N第一带状线和N第二带状线彼此平行并交替，并且对第一周期的各Ls1和Ls2的长度满足等式，第一和第二多通道分配器寻形成在第一介电层的一侧和另外一侧上，以连接彼此平行的N第一带状线和N第二带状线；以及形成在腔的相对方向中的第一和第二中心端口，每个馈电回路部分连接到第一和第二多通道分配器；以及第一和第二槽部分，所述第一和第二槽部分通过对第二屏蔽层形成模式而形成，其中M第一和M第二槽沿着X轴的方向安置，各第一和第二槽分别形成N行第一和N行第二槽阵列，其对每个横过第一和第二带状线，第一槽和第二槽彼此正交。

Yeaah It's something that you need to have Cause when she leave yo *** she gone leave with half 18 years, 18 years And on her 18th birthday he found out it wasn't his Now I aint sayin she a gold digger (When I'm Need) But she aint messin wit no broke Now I aint sayin she a gold digger (When I'm need) but she aint messin wit no broke get down girl go head get down get down girl go head get down get down girl go head get down get down girl go head [Verse 3:] Now I aint sayin you a gold digger you got needs You dont want ya dude to smoke but he can't buy weed You got out to eat and he cant pay yall cant leave There's dishes in the back, he gotta roll up his sleeves But why yall washin watch him He gone make it into a Benz out of that Datson He got that ambition baby look in his eyes This week he moppin floorz next week it's the fries So, stick by his side I know this dude's ballin but yea thats nice And they gone keep callin and tryin But you stay right girl But when you get on he leave yo *** for a white girl Get down girl go head get down Get down girl go head get down get down girl go head get down get down girl go head can I hear that back

yeaah 它的东西，你需要有原因当她离开摇摇***她离开了一半 18岁， 18岁和她的18岁生日，他发现这不是他我现在是不是sayin她一条金挖掘机但她不是messin束手无策，没有发生我现在是不是sayin她一条金挖掘机但她不是messin束手无策，没有发生趴下去的女孩头部着手趴下去的女孩头部着手趴下去的女孩头部着手趴下去的女孩头部 [诗3： ] 我现在是不是sayin你一个黄金挖出你的需要您不希望亚dude吸烟，但他无法购买杂草你失控，吃和他cannot支付yall cannot离开还有的菜在后面，他的宝贝，卷起他的衣袖但为什么yall washin观赏他他经历了使它成为一个奔驰摆脱这种datson 他认为，野心婴儿看在他眼中这个星期，他moppin floorz下周的薯条因此，坚持在他身旁我知道这dude的ballin ，但雅这就是尼斯和他们保持了callin和tryin 但你留在女童的权利但是，当你得到关于他离开摇摇***一个白人女生趴下去的女孩头部着手趴下去的女孩头部着手趴下去的女孩头部着手趴下去的女孩头部

The rain is knockin at my heart and I can't see the sun no more I can't live without you my sun if I'm ever gonna get back picture this You got a kid in a room his hands sweatin and shakin while he's lacin his shoes the excitement's buildin up for what's comin up soon To get there and do what he was born to do He's listenin to the beats He's trying to get in the zone He's gotta take control and make every moment his own just a little bit tired he is a little bit scared but he's so focused he won't show it when he's finally there Cuz now he's out on the floor And he's catchin the pass slashin to the glass, movin so fast, right to the hole, they don't know how to stop him and he keeps on droppin 2's and 3's by the second and the crowd is rockin so they knock him knock him down to the ground before he's crowned as the best He takes a deep breath before he gets up and looks around Everything's in slow motion The crowd is an ocean Only one minute left The rain is knockin at my heart and I can't see the sun no more I can't live without you me sun if I'm ever gonna get back, get back, get back The rain is knockin at my heart and I can't see the sun no more Ican't lice without you my sunif I'm ever gonna get back Imagine A little girl in the stands the kid's her older brother and she's his number one fan He's always lookin out for her and she looks up to him and she wants nothin more than for her brother to win But the truth is that there's someone on the other team Someone who stole her heart Someone who holds her dreams They kiss for the 1st time just the other evening Her brother's 23 but her lover's number 16 The drama is set the whole scene is intense she doesn't know which one to cheer for and she's gettin upset 23 on the chase cuz they're at each other's necks and what happens next Her boyfriend steals the ball and checks the kid in the chest 16 on the break Brother goes in the air with brother up in his face They come crashing to the dirt Tears about to burst When the game is over who she gonna run to first, yo The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back, get back, get back The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back 有时感觉阻碍太多但我还是为了我的梦想而奋斗 Nothin's gonna stop me Ey yo this game of life is one big struggle We gotta hustle just to make it everyday to fight the fears we facin and grab those dreams we chasin cuz there ain't no competition when we rise to the occasion c'mon The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back, get back, get back The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back 注:IN可以看作是ING如FACIN可以看作facing

雨是敲入我的心我看不到太阳不再我不能没有你我的太阳如果我曾经去买回来此图片你有一个孩子在一个房间里他的手sweatin和沙金，而他的拉欣他的鞋兴奋的buildin了什么的，很快就要来要到那里做什么，他出生做他listenin的节拍他试图在该地区获得他得采取控制和利用自己的每一刻只是有点累了他有点害怕但他是如此专注，他将不显示它的时候，他终于有现在的Cuz他在地上他的捉通行证 slashin的玻璃，居无定得那么快，有权向孔，他们不知道如何阻止他他不断droppin 2的和3的由第二摇滚和人群所以他们会把他会把他撞倒在地之前，他的加冕为最佳他深吸了一口气，才起身环顾四周一切都在慢动作人群是一个海洋仅1分钟雨是敲入我的心我看不到太阳不再我不能没有你我的太阳如果我曾经去买回来，回来，回来雨是敲入我的心我看不到太阳不再没有你我的sunif Ican't虱子我曾经去买回来想像看台上的一个小女孩这个孩子是她的哥哥，她是他的头号球迷他总是在找谁了她和她抬起头来他和她想没什么比她的哥哥更赢得但是，事实是，对其他团队的人有人谁偷了她的心有人谁拥有她的梦想他们只是亲吻对方的第一时间为晚上她的弟弟，但她的爱人23的16号该剧设置整个场面激烈她不知道哪一个加油，她的gettin不高兴 23大通他们的Cuz在对方的脖子和接下来会发生什么她的男朋友抢断球，并检查孩子的胸部 16个突破哥哥和弟弟去他的脸在空中他们来到崩溃的污垢即将爆裂泪当比赛结束谁，她要去跑第一，溜溜雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来，回来，回来雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来有时感觉阻碍太多但我还是为了我的梦想而奋斗没办法，拢不惊安永哟这样的生活游戏是一个大斗争我们得喧嚣这只是为了日常战斗的恐惧，我们都会面临的两个并抓住那些梦想，我们查辛没有的Cuz没有竞争当我们挺身而出来吧雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来，回来，回来雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

This time, we do not want to colorize the image. I've dragged my Hue slider to the left to a value of -123

这次不要勾选着色，我将色相的滑标向左移到-123

A timely report from an independent prosecutor would surely be helpful in buttressing and justifying that order.

及时报告从一个独立的检察官一定会有助於支撑和合理的秩序。