查询词典 get into lines

When we get to the end, and the confession, it is as if the two time-lines fuse, and past and present explode into a single moment.

当我们读到结尾，读到小说主人公招供那一刻，两条时间线好像熔合在一起，过去与现在同时爆发的一刻。

Teacher:get into lines boys.good.now we can do some exercises.

老师：孩子们，排好队。好，现在我们做操。

If anything the conference is TOO successful, as the lines to get into the parties are really starting to impact my ability to make those valuable business connections.

当这些派队得到了足够的支持并且开始影响我做那些有价值的商业联系时，这个会议就太成功了。

Teacher:Get into lines boys.

老师：孩子们，排好队。

Lines to get into the park started forming in the afternoon.

格兰特公园一开门，支持者奔向主席台。

Get into lines, hand in hand.

手拉手，牌号对。

If you are outside, get into the open, away from buildings and power lines.

如果你在室外，进入开放的，远离建筑物和输电线路。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The part that I'm taking on isn't exactly clear at the moment. I know I will be one of the ensemble..and possibly get a few lines here and there....The show already has it's lead roles casted and the other roles are not suitable for my age. But, I don't mind...it will be the first time that I actually get to be a part of a professional Broadway production! Everything is coming from NY!!! And the original writer is going to be the director! So, I know that this will be my chance to learn and make my way into the industry. Hopefully, next time, there will be a bigger role for me to play.I'm so thankful that God has been leading me into the right direction. I am so blessed with opportunities...and I know that my patience is finally paying off. I'm very excited to be able to train more in tap dancing because I have always wanted to improve in that area....it's going to be a lot of hard work..but, I'm so looking forward to it. I do feel very sorry for my fans who have been walking with me since the beginning of the competition. They have been so supportive of me and it made me so sad to see them cry when I made my decision. I hope that they will not be upset with me because of it. I hope that they will see that this choice will only help my future...and I hope that they will continue to support me even though my participation with the competition has come to an end. It really touched me to see that they cared so much.

我知道我可能只是其中一个群舞，或许会有点台词，因为他们早就订了女主角人选，另一个女角年纪比较大，并不合适我，但是我不在乎…这是我的第一次参于真正百老制作的演出，每一个成员都是从纽约来的，原编剧将会亲自来导演这次演出，所以我很清楚这是我最好的学习机会，也是给我提供了一条通向百老的捷径之路，下一次我一定会争取到一个很好的角色…我感激我的上帝指引我一个正确的方向，我非常感激有这麼好的一个机会，我等了那麼久，总算有了一个好的起步…我特别兴奋的是，我这下子总算有机会好好学"踢躂舞"了，这是我最弱的一种舞，我一直希望能把它学好，尽管我知道会很辛苦，但是我已经作好了思想准备…还有对那些从一开始就支持我的那些粉丝，我感到非常抱歉，尤其是当我在台上说出我离去的决心时，看到她们一个个都哭得那麼伤心，我也很难过，我希望她们能明白，这个决定是绝对对我前途有利的，我希望就算是我不再比赛，她们仍然会支持我，一直以来，她们对我的关心和鼓励最让我心动的…。

In Mansun's Only Love Song', we get a glimpse into his life as he sees himself during the day - a vicar in appearance, but his mind is far away, dreaming of the exciting world of being a stripper -"Mavis looking sexy through her dress it shines, standing in the vestry she recites her lines" are how he views himself at that time - just reciting lines like an actor, not really meaning it, as his mind was on being Mavis, his stripper alter-ego, who really is "all things to no men" in the clubs.

在Mansun's Only Love Song'这首歌中，我们得以窥见到他的真实生活，他真实的一面----表面道貌岸然，脑子里的幻想却早已九霄云外。。去幻想那些本该不属于自己生活的东西，比如，他会想如果自己变成脱衣舞娘那样的生活会有多刺激--绚丽的灯光下的Mavis--也就是幻想中的自己，看起来是那么迷人与性感。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。