查询词典 get into a hobble

After the eye splashes into, may have the chemosis and the cornea damage, the eye ache, bursts into tears, fears the light, if therefore is not careful splashes into the eye, must flush the ocular region 15 minutes promptly with the percolating water.

眼睛溅入后可产生结膜水肿与角膜损伤、眼睛疼痛、流泪、怕光，因此若不小心溅入眼睛，要及时用流动水冲洗眼部15分钟。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Filaments free, very short; anthers free or mostly connivent into a sheath around ovary, 2 anterior ones with spurlike or wartlike and nectariferous appendages at base, these extending into anterior spur, connectives produced apically into conspicuous, membranous appendages.

花丝离生，非常短；花药离生或多数在子房周围靠合成为鞘，前面2枚基部具距状或瘤状和分泌蜜汁的附属物，这些延伸进入前面的距中，药隔顶部生明显、膜质附属物。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

The invention discloses a method for extracting polysaccharide from chiretta and an application, which comprises breaking dry chiretta via a breaker into powder, arranging into an extraction pot, adding ligarine to reflux and defat, adding distilled water into defatted residues to extract, processing alcohol precipitation and centrifugation to obtain deposits, removing protein, processing acid hydrolysis and gel chromatography separation, freezing and drying to obtain active chiretta polysaccharide.

本发明公开了一种从当归中提取多糖的方法及应用。干燥当归用粉碎机粉成粉末，置入提取罐中，加入石油醚回流脱脂，向脱脂后的残渣中加入蒸馏水提取，醇沉、离心得沉淀，除去蛋白、酸解、凝胶色谱分离、冷冻干燥得到活性当归多糖。

Two kinds of hybrid composite resins and two kinds of polyacid-modified composite resins (Dyract AP and F2000)were made into discal restorations, which were divided into 4 groups and each group were then divided into 5 subgroups.

分别以卡波姆和聚乙烯吡咯烷酮作为漂白剂的增稠载体，以11％和15％的CP作为漂白活性成分，配制漂白剂。

Classified by commodity variety, it can be categorized into the counterfeiting of consuming goods, agricultural goods, architectural materials, medical goods, industrial means of production, publication, paper currency and cultural relics; classified by nature, it can be categorized into fake commodities and substandard commodities; classified by information structure, it can be categorized into the counterfeiting of symmetric information and dissymmetric information.

按照商品品种分类，可以分为消费品类假冒伪劣、农资类假冒伪劣、建筑类假冒伪劣、药品类假冒伪劣、工业生产资料类假冒伪劣、出版类假冒伪劣和纸币文物类假冒伪劣；按照性质分类，可以分为假冒商品、伪劣商品；按照信息结构分类，可以分为不对称信息的假冒伪劣、对称信息的假冒伪劣。

High-protein flour, whole wheat flour, salt and sugar mixture poured into the stainless steel bowl, dig a middle-dug pits, water poured into the pumpkin yeast solution, then add pumpkin mud, use low-speed mixing into a smooth in the rest of the flour gradually,until the dought product gluten.

3：高筋面粉、全麦面粉、盐和糖混合倒入搅拌缸，中间挖一个坑，倒入南瓜水酵母溶液，再加入南瓜泥，先用低速搅拌成团，至光滑。再逐步加入剩下的面粉，至筋性产生。

Into the clubs, the air was filled with four thick scent of essential oils, into the restaurant to hear the gurgling water and soothing music, into which Dungan away from the city hustle and bustle.

走进会所内空气中四处弥漫着浓浓精油的香味，走进店内听到的是淙淙流水和舒缓音乐，步入其中顿感远离都市喧嚣。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48