查询词典 germplasm

Genetic Integrity of 20 Barley (Hordeum vulgare L.) Germplasm Resources Based on Hordein Analysis

基于醇溶蛋白的20份大麦种质资源遗传完整性分析，王芳；卢新雄；马晓岗；迟德钊；张志娥；陈晓玲-66文免费论文库，大学生论文库

The germplasm resources are rich and the varieties are wide in Juglans, which are of important biological significance and economic value.

核桃属植物种质资源丰富，品种繁多，具有重要的生物学意义和经济价值。

To accumulate basically materials of the medicinal plants from Lamiaceae in Henan, and provide data for its utilization, conservation, artificial reproduction and enlarging population of wild resources, The germplasm resource and floristic phgtogeographical features of medicinal plants from lamiaceae in Henan are analyzed by conducting field investigation and consulting specimens and related data.

为了积累河南省唇形科药用植物资源的基础资料，以便为其野生资源的开发利用、保护以及引种驯化提供科学依据，此次研究采用野外踏查、室内查对腊叶标本和校对相关文献资料的方法，对河南省唇形科药用植物种质资源及其区系特点进行了初步的研究和探讨。

The objective of this work is to find the relationship among accessions of leucaena from different geographical distribution based on diversity analysis so as to provide theoretical foundation for the evaluation, classification, utilization and selection of leucaena germplasm resources.

本研究旨在分析银合欢种质遗传多样性的基础上，探讨不同来源银合欢种质间的关系，寻找遗传差异，为银合欢种质资源的评价、分类、利用和选育种提供理论依据。

The study on germplasm resource of Lonicera Caerulea L. was not much made abroard and many problems should be resolved.

国外对蓝靛果忍冬种质资源的研究时间也较短，尚不深入，许多问题尚待解决。

First established a germplasm pool of Lonicera Caerulea L in China and provided valuable breeding materials for future breeding work.

在国内首次建立了蓝靛果忍冬种质资源圃，为将来的育种工作提供了非常宝贵的材料。

Europe and North America ; barley ; malting barley ; germplasm resources ; utilization ; evaluation

欧洲和北美洲；啤酒大麦；种质资源；利用；评价

In this context the wild mango germplasm at Lapuo County, Guangxi, China were studied by using their biological characteristics and the amplified fragments length polymorphism molecular marker. These wild mangoes were found morphologically similar to Mangifera indica; and the AFLP markers showed that at the similarity coefficient of 0.63, 92 mangoes including cultivars, Mangifera sylvatica Roxb., wild mangoes, Mangifera. Siamensis Warbg. were divided into 14 groups and that the wild mangoes at Lapuo were close to M. sylvatica Roxb. and M. siamensis Warbg., but remained to fall in the middle of the cultivars and could not be separated from them.

结果表明：那坡县野生芒果在形态学上与普通芒果较为相似；AFLP标记显示，包括部分栽培品种、林生芒果、广西百色那坡县野生芒果、泰国芒果等在内的92份芒果种质在相似系数0.63的水平上可分为14组，广西百色那坡县野生芒果虽然与林生芒果、泰国野生芒果等芒果属其它种较为接近，但仍处于栽培芒果的中间，不能与栽培芒果分开。

So it is very necessary to study variances of Chinese Meretrix meretrix germplasm resources and their molecular genetic bases for their lasting utilization,sustained and healthy development of aquaculture industry. In this study, the genetic bases and their variances of representative populations of Meretrix clam were investigated through phenotypic traits and on the molecular level by quantitative genetic methods and molecular marker analysis technique.

本研究从表观性状和分子水平上探测我国文蛤种质资源的遗传基础和变异水平，旨在深入了解我国文蛤的种质状况并为其保护和可持续利用提供依据；在此基础上，通过杂交选择育种实践，分析和探讨文蛤由此获得杂种优势水平，为养殖文蛤的遗传改良和新品种培育提供理论指导。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001～0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点，远多于S群体(14)和G(21)群体，而且在53个特有位点中有9个出现频率为100%的位点，这些位点可以作为区分其它2个群体的特征性标记；S– G群体特有的位点有112个，其中有4个位点出现频率为100%，可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585，遗传距离只有0.0424，在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起，说明二者的亲缘关系很近，应属于种内群体间的关系；而W与S和G的遗传相似性系数均较小(0.7939和0.7941)，相对遗传距离很大而且十分相近(0.2308和0.2305)，在亲缘关系树状图上单独分出一支，也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现，W的ITS序列长度在1266-1269 bp，而S和与G的ITS序列总长度分别为1520 bp和1614 bp；从ITS1和ITS2长度来看，W分别为739-741 bp和316-317 bp，S为895 bp和414 bp，G为987 bp和416 bp；而从ITS碱基组成来看，W的GC含量在62.32-62.62%之间，而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003，S与G群体间的遗传距离是0.010，说明W群体内变异很小，而S与G群体间已出现明显的遗传分化，但还均属于种内群体间的遗传变异；而W与G和S的遗传距离分别达到0.110、0.147，两个类群差异显著，已远超出种内群体间的遗传变异。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失