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We hypothesize that Zfp474 functions as a germ cell specific transcription factor that plays important roles in spermatid differentiation and oocyte development.

结果提示：Zfp474基因对精子发生和卵母细胞的发育可能起重要作用。

In the examination of immunofluorescence using the polyclonal antisera against centrin to detect the expression of centrins in the rat male germ cells, it was found that the fluorescent centrin was localized on centrioles juxtanuclear position in the early round spermatid stage.

用制备的Centrin多克隆抗体，对大鼠生精细胞进行免疫荧光染色，可见呈荧光的Centrin蛋白定位于细胞核近旁的中心粒上。

Oocyte, spermatocyte, implying the important role silkworm vasa plays in the early development of embryos and the differetiation of germ line.

原位杂交结果提示，家蚕vasa基因在家蚕早期胚胎发育和生殖系的分化形成中发挥重要作用。

Immunohistochemistry for Bax: Bax expression was detected in germ cell cytoplasm cytoplasm and nucleus of seminiferous tubule theca cell, obviously spermatocyte.

免疫组化观察Bax的表达情况：Bax表达见于生精细胞的胞质和胞核，以精母细胞为主。

AR expressed stronger in spermatocytes and spermatogenic cells, ER in germ cells and sperm expressed strong in the second spermatocyte, PR in Leydig cells also expressed stronger.

AR在精母细胞和精细胞中表达较强，ER在精细胞和精子中表达较强，精母细胞中次之，PR在睾丸间质细胞中表达较强。

Immunohistochemistry for HIF-1α: HIF-1αexpression was detected in germ cell cytoplasm cytoplasm, obviously spermatocyte,and was detected in nucleus of seminiferous tubule theca cell too.

免疫组化观察HIF-1α的表达情况： HIF-1α表达主要见于生精细胞的细胞质，以精母细胞明显，也见于曲细精管外周膜细胞的胞核。

The histosection of ram testes about 2-3 month old were made and observed, the morphology of germ cells were uniform in seminiferous tubules and did not enter the spermatogenesis. We have found a lot of PGP9.5 positive spermatogonium on basal membrane and it could be used culture in vitro.

对2~3月龄的山羊睾丸做切片观察，发现曲细精管中细胞形态比较单一，生精上皮还没有进入生精周期，通过免疫组化染色发现在曲细精管基底膜有大量PGP9.5 精原干细胞。

The first 0.1%collagenaseⅣ 0.1% hyaluronidase for 20 min and than 0.25% trypsin 0.04% EDTA 5μg/mL DNaseⅠfor 5 min,its enough to obtain germ cells and have optimal cell viability. 8.4±2.6×106 cells per gram testis were isolated and there was(19.7±5.3)% of PGP9.5 spermatogonium and (23.8±3.6)% of c-kit spermatogonium.

采用两步酶消化法，0.1%胶原酶和0.1%透明质酸酶消化20 min，0.25%胰酶 0.04% EDTA 5μg/mL DNaseⅠ处理5 min，就可以使睾丸生殖细胞充分分离处出来，每克睾丸组织克获得8.4±2.6×106个细胞，其中PGP9.5 精原细胞占(19.7±5.3)%，c-kit 精原细胞占总数的(23.8±3.6)%。

Primordial germ cells were the progenitor cell of spermatozoon and ovum.

原始生殖细胞是精子和卵子的前体细胞。

Spermiogenesis is a crucial process for germ cell formation, which ensures the efficient transmission of the paternal genome by the spermatozoa to the ooctyte at fertilization.

它包括染色质的压缩、顶体和鞭毛的形成以及去除多余的细胞质，是精子发生的一个重要环节，确保了成熟精子具有正确的形态和完整的功能。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！