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An experiment was performed to compare effect of different cell media on embryonic germ cells derived from primordial germ ridge of Kunming albinotic strain mouse.

以昆明白品系小鼠胎儿生殖嵴为材料,以不同的培养液分离培养胚胎生殖细胞,发现小鼠胎儿肝细胞条件培养液的效果好于未添加白血病抑制因子的基础培养液,而差于添加了1 000 IU/mL LIF的基础培养液。

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要:通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt;采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中,然后将注射存活的受精卵植入假孕母鼠的输卵管;取其产3 wk龄子代鼠进行PCR初选,再通过Southern杂交进一步确证;利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

Subsequently,germ elongation ceases and the tip of the germ tube swells to form an appressorium surrounded by a thick melanized cell wall.

稻瘟病菌是引起水稻重要病害稻瘟病的病原菌,也是研究丝状真菌的重要模式生物。

Embryonic germ cell lines were isolated and cloned from gonad,gonadal ridge,analogue or the surrounding tissues of 9.5~13.5 days postcoitum mouse fetuses.Primordial germ cells were co-cultured with mouse embryonic fibroblasts obtained from the same conceptus.

自交配后9.5~13.5 d小鼠胎儿生殖嵴/腺或其类似物及周围组织,采用与其同源胎儿成纤维细胞共培养的方式分离克隆到具小鼠ES细胞诸多特征的胚胎生殖细胞系。

In order to investigate the changes of HSF2 mRNA expression and its role in germ cell apoptosis induced by heat stress or a high of testosterone undecanoate,we established the operation induced unilateral cryptorchid and TU injected monkey models,and examined in situ analysis of testicular cell DNA fragmentation and the expre ssion level of HSF2 mRNA in germ cells after treatment.

为了探讨HSF2mRNA在热应激和超生理剂量睾酮诱导恒河猴生精细胞凋亡中的表达变化,我们建立了手术诱导单侧隐睾和注射大剂量 11酸睾酮恒河猴动物模型,应用 3′末端标记分析和原位杂交方法,检测睾丸细胞的凋亡信号和HSF2的表达变化。

The laboratory information system collection disease germ material can describe the condition development accurately. For Example germ cluster and regional setting and so on. An urgent need that Nosocomial Urinary Tract Infection will be dected from the laboratory report.

而实验室资讯系统收集的病原菌资料更能准确描述病情的发展,如病原菌种的群集和区域位置等,若能由实验室报告提早侦测出有院内泌尿道感染实在有迫切必要。

Germ potential is established after conception in a totipotent zygote and retained subsequently during development in blastomers of morula , cells form the inner cell mass of blastocyst , epiblast and population of primordial germ cells .

种子潜能的确定是全能的受精卵形成之后,并且随后在桑葚胚,胚泡内层细胞,外胚层和原生殖细胞的发育过程中保留。

Generally say, the oral hygiene is bad, tartar and dental calculus pile up, the food Qian fills, germ and germ spot function, badly fill dental cavity and have a tooth filled incitement, or oppress gum etc., is all important partial reason that causes a tooth to blaze for week.

一般说,口腔卫生不良,牙石和牙垢堆积,食物嵌塞,细菌和菌斑作用,不良补牙和镶牙刺激,或压迫牙龈等,都是引起牙周炎的重要局部原因。

In addition, germ-line epimutations of other tumor suppressor genes, such as MSH2 and BRCA1, have also been reported, demonstrating that epimutations might arise in the germ-line (during gametogenesis or early embryonic development).

随后,关于其他抑癌基因MSH2与BRCA1等的胚系异常甲基化也陆续有报道。这提示,表观突变也可以起源于胚系(生殖细胞形成期或胚胎发育早期),从而造成全身细胞广泛的基因转录沉默。

Easily store food broken bits inside the gums ditch, adhere to a germ spot, become the good district that the germ growth breeds, because it presents to close appearance, don't easily sweep and often cause the gum burning disease and tooth the paroxysm occurrence is for week.

龈沟内易积存食物碎屑,附着菌斑,成为细菌生长繁殖的良好区域,由于其呈封闭状态,不易清洁,常导致牙龈炎症及牙周疾病的发生。

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推荐网络例句

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入,与管线26相通,与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。

For he that is now called a prophet, in time past was called a seer.

他们就往天主的人所住的城里去了。