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To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

Purpose:To study the expression of induced nitric oxide synthase and testicular germ cellapoptosis in experimental cryptorchidism.

目的：探讨隐睾中诱生型一氧化氮合成酶基因表达与生殖细胞凋亡的关系。

Supplements : You can add the following to the dry food/bedding: wheat germ, finely ground egg shells or cuttlebone, soybean meal, Wombaroo insectivore mix, fish flakes, fine mouse cubes, bone meal, graham flour, and dry brewer's yeast provides proteins and trace elements essential to the insects' growth and makes larvae grow more.

副刊 ：你可以添加以下到干粮/寝具：小麦胚芽，细磨鸡蛋壳或海螵蛸，豆粕， wombaroo食虫植物的组合，鱼薄片，罚款鼠标立方体，骨粉，格雷厄姆面粉，干燥啤酒酵母（提供蛋白质和微量元素对于以昆虫生长发育，使幼虫成长更多。

To produce appropriate data format of ANFIS, the configuration of trusses is described as germ cluster, which can be compressed as decimal fraction by binary technology.

为使系统计算更加精确，还应用了基因分片的方法，将一个长基因串分成若干段相对较短的基因片段。

Thepropertiesof rice germ protein including dissolvability,thermal denaturalization,and emulsification,were studied.

大米胚芽是大米加工的副产品，营养丰富，具有很高的利用价值。

Adopting efficient new adsorption material, ACF mask has properties as: gas defense, deodorize , germ filter, dust preventer and etc.

活性碳纤维口罩采用新型高效的吸附过滤材料，具有防毒、除臭、滤菌、阻尘等功效。

The results showed that(1) TES accelerated the meiosis and maturity of germ cells in leptotene, zygotene, pachytene, diplotene and diakinesis of prophase 1 of spermatocytes while STI inhibited the meiosis and maturity,(2) there was a significant e...

结果表明：(1)TES对前期Ⅰ5个分期的生殖细胞具有促进细胞分裂和成熟的作用，STI抑制细胞分裂和成熟；(2)TES和STI对前期Ⅰ粗线期药物效应显著；(3)TES和STI在小鼠体内最大有效效应分别持续约计70和50小时。

Enzymatic activity assay of oxalate oxidase in transgenic tobacco plants indicated that the germ in gene from monocotyledon, could be expressed in dicotyledon tobacco plants that resu;ted in the formation of homopentameric protein with enzymatic activity correctly.

酶活性检测表明，源于单子叶植物小麦的germin基因能够在双子叶植物烟草中表达并正常装配成具有酶活性的同源五聚体蛋白。

Pathogens were diplococcus, nongonococcal germ, mycoplasma urealytium and chlamydia trachomatis and the rate was 5.72%、73.77%、14.25% and 6.26% respectively.

病原体为淋球菌、非淋球菌细菌、解脲支原体和沙眼衣原体共四类，分别为5.72%、73.77%、14.25%和6.26%。

What is the relationship between the doctors and the disease germ?

究竟医生与疾病的关系是甚麼？

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。