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An experiment was performed to compare effect of different cell media on embryonic germ cells derived from primordial germ ridge of Kunming albinotic strain mouse.

以昆明白品系小鼠胎儿生殖嵴为材料,以不同的培养液分离培养胚胎生殖细胞,发现小鼠胎儿肝细胞条件培养液的效果好于未添加白血病抑制因子的基础培养液,而差于添加了1 000 IU/mL LIF的基础培养液。

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要:通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt;采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中,然后将注射存活的受精卵植入假孕母鼠的输卵管;取其产3 wk龄子代鼠进行PCR初选,再通过Southern杂交进一步确证;利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

Subsequently,germ elongation ceases and the tip of the germ tube swells to form an appressorium surrounded by a thick melanized cell wall.

稻瘟病菌是引起水稻重要病害稻瘟病的病原菌,也是研究丝状真菌的重要模式生物。

Embryonic germ cell lines were isolated and cloned from gonad,gonadal ridge,analogue or the surrounding tissues of 9.5~13.5 days postcoitum mouse fetuses.Primordial germ cells were co-cultured with mouse embryonic fibroblasts obtained from the same conceptus.

自交配后9.5~13.5 d小鼠胎儿生殖嵴/腺或其类似物及周围组织,采用与其同源胎儿成纤维细胞共培养的方式分离克隆到具小鼠ES细胞诸多特征的胚胎生殖细胞系。

In order to investigate the changes of HSF2 mRNA expression and its role in germ cell apoptosis induced by heat stress or a high of testosterone undecanoate,we established the operation induced unilateral cryptorchid and TU injected monkey models,and examined in situ analysis of testicular cell DNA fragmentation and the expre ssion level of HSF2 mRNA in germ cells after treatment.

为了探讨HSF2mRNA在热应激和超生理剂量睾酮诱导恒河猴生精细胞凋亡中的表达变化,我们建立了手术诱导单侧隐睾和注射大剂量 11酸睾酮恒河猴动物模型,应用 3′末端标记分析和原位杂交方法,检测睾丸细胞的凋亡信号和HSF2的表达变化。

The laboratory information system collection disease germ material can describe the condition development accurately. For Example germ cluster and regional setting and so on. An urgent need that Nosocomial Urinary Tract Infection will be dected from the laboratory report.

而实验室资讯系统收集的病原菌资料更能准确描述病情的发展,如病原菌种的群集和区域位置等,若能由实验室报告提早侦测出有院内泌尿道感染实在有迫切必要。

Germ potential is established after conception in a totipotent zygote and retained subsequently during development in blastomers of morula , cells form the inner cell mass of blastocyst , epiblast and population of primordial germ cells .

种子潜能的确定是全能的受精卵形成之后,并且随后在桑葚胚,胚泡内层细胞,外胚层和原生殖细胞的发育过程中保留。

Generally say, the oral hygiene is bad, tartar and dental calculus pile up, the food Qian fills, germ and germ spot function, badly fill dental cavity and have a tooth filled incitement, or oppress gum etc., is all important partial reason that causes a tooth to blaze for week.

一般说,口腔卫生不良,牙石和牙垢堆积,食物嵌塞,细菌和菌斑作用,不良补牙和镶牙刺激,或压迫牙龈等,都是引起牙周炎的重要局部原因。

Stage I of both male and female stone flounder appears only once in all its life, and the germ cells are comprised by spermatogonia or oogonia. The gonad from Mar to Aug keeps at stage II. The gonad index of testis at this stage is 0.037%, and the amount of spermatogonia is increased quickly. There is some linear germ plasm in the cytoplasm of spermatogina. In ovary at this stage it is mostly composed by oocyte of phase 2 which the character is the appearance of yolk nucleus, and no zona radiate in membrane. The mean GI of ovary is 1.95%. From Sep to Oct gonad is at stage III which testis is composed by lots of spermatogina and few spermatocytes, and the mean GI of testis at this stage is 0.086%. In ovary the ooctyes at phase 3 are in dominate position, the yolk nucleus disappear. And the GI of this stage is 3.35%. Both testis and ovary are at stage IV in Nov. hi testis the germ cells are in spermiogenesis, and the mean GI is 0.93%. hi ovary the oocytes are mostly at phase 4, which are filled in the cytoplasm with vitellin granule, and the zona radiate in membrane begins to formation. Nucleus moves to one side of the oocyte gradually. The mean GI of ovary at this stage is 9.37%.

在每年的3月-8月期间性腺处于Ⅱ期,此期精巢中精原细胞明显增多,胞质局部可见有线状的生殖质存在,平均成熟系数为0.037%;卵巢中以2时相卵母细胞为主,可见细胞质中出现强嗜碱性的卵黄核,细胞外由一层滤泡细胞包围,但尚无放射带,平均成熟系数为1.95%。9月-10月期间性腺处于Ⅲ期,此期精巢中仍有大量精原细胞,同时可见部分精母细胞,平均成熟系数为0.086%;卵巢中以3时石鲜孟加限加$玩印面n洲匆s性腺发生、分化及发育的周年变化相卵母细胞为主,细胞质中的卵黄核己消失,平均成熟系数为3.35%。11月性腺处于IV期,此期精巢内精细胞正处于不同的形成过程中,平均成熟系数为0.93%;卵巢中以4时相卵母细胞为主,胞质中充满染成桔红色的卵黄颗粒。

The result showed Indomethacin inhibited the formation of germ tube and hypha of candida albicans by dose dependence: indomethacin can inhibit 50% germ tube formation at 0.75mM level, while it can fully inhibit germ tube formation at 2.0mM level.

结果表明消炎痛对白色念珠菌芽管和菌丝形成的抑制作用与剂量相关:消炎痛浓度为0.75 mM时可抑制50%芽管形成,浓度为2.0mM时可完全抑制芽管和菌丝形成。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。