查询词典 germ-disease

Therefore, fluorescein isothiocyanate conjugated lectins, namely concanavalin agglutinin, wheat germ agglutinin and soybean agglutinin, were used to localize lectin receptors on plasma membrane of male and female gametes and other relevant sexual cells, as well as embryos of various developmental stages of Nicotiana tabacum and Torenia fournieri by cooled charge coupled device and Confocal Laser Scanning Microscope.

从而证实：在本实验中用的几种酶组合对凝集素受体的影响并不大，即由酶解及解剖得到的雌性细胞的荧光在分布形式及强度上没有明显的差异；对比原位酶解与离体酶解得到的雌性细胞的荧光，证实机械操作对荧光的分布形式及强度也没有明显的影响。

METHODS AND RESULTS: We targeted the gene for proapoptotic Nix, which is transcriptionally upregulated in pressure overload and Gq-dependent hypertrophies, in the mouse germ line or specifically in cardiomyocytes and conditionally overexpressed it in the heart.

方法和结果：促凋亡基因Nix在压力超负荷和Gq依赖的肥厚中表达上调，我们在小鼠中特异性的敲除Nix基因、或将其转导入小鼠心脏使之过表达。

Conciusion: Modified Mallory's trichrome staining method may be used to study the mineralization of dental germ.

改良Mallory's三色法较HE染色更为清楚的观察到牙胚发育中组织矿化过程的变化。

In the present paper, the Sox gene expression analysis of different tissues from the Trionyx sinensis was studied by using RTPCR, and Sox gene fragments of expression from the testicle, brain and spleen were cloned using RTPCR products. The results show that Sox gene has specific expression in the testicle, brain, spleen, cardiac muscle and kindney and hasn't expression in muscle, liver and ovary. The results of clone reveal that the Sox genes of expression in the testicle are TSSox1 and TSSox 4, and those are TSSox2 and TSSox4 in the brain, and that is TSSox4 in the spleen. This suggests that the Sox gene act important role not only on the sex determination, but also on the development of neural system, immunocyte system and the differentiattion of male germ cell.

本文采用RT—PCR技术，研究了中华鳖不同组织Sox基因的表达，并通过PCR直接克隆法，分析了来自睾丸、脑和脾组织中的Sox基因序列结果表明在中华鳖的成体组织中，Sox基因在脑、心肌、肾、脾和雄性的睾丸组织中均有不同程度的表达，而在肌肉、肝脏和雌性的卵巢中则无表达，显示该基因具有组织表达特异性克隆分析显示，在睾丸组织中表达的是TSSox1和TSSox4基因，而在脑组织中表达的是TSSox2和TSSox4基因，脾组织中表达的是TSSox4基因此结果表明Sox基因不仅在性别决定中起作用，还可能在神经系统、免疫系统多种组织中起重要作用

Vasa is one of the fateful elements of germ line, which is conserved widely in many species.

vasa是生殖系决定重要成分之一，具有广泛的保守性。

In conclusion, silkworm vasa plays an important role in the determination of the germ line. What's more, the optimization of ISH has been tested.

此外，在进行原位杂交的研究中，我们探索和建立了家蚕胚胎原位杂交的方法，并对其中关键的影响因素进行了优化。

Among the DEAD-box proteins, VASA and PL10 are required for germ cell formation.

其中的vasa和PL10基因对于生殖细胞的发生具有重要作用。

In conclusion, silkworm vasa plays an important role in the determination of the germ line.Whats more, the optimization of ISH has been tested.

此外，在进行原位杂交的研究中，我们探索和建立了家蚕胚胎原位杂交的方法，并对其中关键的影响因素进行了优化。

The ISH results show that silkworm vasa expresses only in germ cell lines e.g.

家蚕vasa基因在发育胚胎的生殖系存在部位有明显的杂交信号。

Vasostomy can reverse germ cell apoptosis due to Vasoligation; Bcl-2 gene inhibited apoptosis of spermatogenic cells; Vasoligation and Vasostomy have a effect on transcript level regulation of Bcl-2 gene

大鼠输精管阻断所致的生精细胞凋亡加剧现象是可复的，阻断解除后生精细胞凋亡逐渐减轻并恢复至阻断前水平；Bcl-2基因在输精管复通后生精细胞凋亡减轻中发挥了抗凋亡作用；输精管阻断及复通过程中存在Bcl-2基因转录水平的调节。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！