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Oocyte, spermatocyte, implying the important role silkworm vasa plays in the early development of embryos and the differetiation of germ line.

原位杂交结果提示,家蚕vasa基因在家蚕早期胚胎发育和生殖系的分化形成中发挥重要作用。

Immunohistochemistry for Bax: Bax expression was detected in germ cell cytoplasm cytoplasm and nucleus of seminiferous tubule theca cell, obviously spermatocyte.

免疫组化观察Bax的表达情况:Bax表达见于生精细胞的胞质和胞核,以精母细胞为主。

AR expressed stronger in spermatocytes and spermatogenic cells, ER in germ cells and sperm expressed strong in the second spermatocyte, PR in Leydig cells also expressed stronger.

AR在精母细胞和精细胞中表达较强,ER在精细胞和精子中表达较强,精母细胞中次之,PR在睾丸间质细胞中表达较强。

Immunohistochemistry for HIF-1α: HIF-1αexpression was detected in germ cell cytoplasm cytoplasm, obviously spermatocyte,and was detected in nucleus of seminiferous tubule theca cell too.

免疫组化观察HIF-1α的表达情况: HIF-1α表达主要见于生精细胞的细胞质,以精母细胞明显,也见于曲细精管外周膜细胞的胞核。

The histosection of ram testes about 2-3 month old were made and observed, the morphology of germ cells were uniform in seminiferous tubules and did not enter the spermatogenesis. We have found a lot of PGP9.5 positive spermatogonium on basal membrane and it could be used culture in vitro.

对2~3月龄的山羊睾丸做切片观察,发现曲细精管中细胞形态比较单一,生精上皮还没有进入生精周期,通过免疫组化染色发现在曲细精管基底膜有大量PGP9.5 精原干细胞。

The first 0.1%collagenaseⅣ 0.1% hyaluronidase for 20 min and than 0.25% trypsin 0.04% EDTA 5μg/mL DNaseⅠfor 5 min,its enough to obtain germ cells and have optimal cell viability. 8.4±2.6×106 cells per gram testis were isolated and there was(19.7±5.3)% of PGP9.5 spermatogonium and (23.8±3.6)% of c-kit spermatogonium.

采用两步酶消化法,0.1%胶原酶和0.1%透明质酸酶消化20 min,0.25%胰酶 0.04% EDTA 5μg/mL DNaseⅠ处理5 min,就可以使睾丸生殖细胞充分分离处出来,每克睾丸组织克获得8.4±2.6×106个细胞,其中PGP9.5 精原细胞占(19.7±5.3)%,c-kit 精原细胞占总数的(23.8±3.6)%。

Primordial germ cells were the progenitor cell of spermatozoon and ovum.

原始生殖细胞是精子和卵子的前体细胞。

Spermiogenesis is a crucial process for germ cell formation, which ensures the efficient transmission of the paternal genome by the spermatozoa to the ooctyte at fertilization.

它包括染色质的压缩、顶体和鞭毛的形成以及去除多余的细胞质,是精子发生的一个重要环节,确保了成熟精子具有正确的形态和完整的功能。

The results showed short peptide LD-1 can have a well inhibiting effect on the sporangium germination of Pseudoperonospora cubensis and have a certain teratogenic action on the development of the germ tube of sporangium.

结果表明:短肽LD-1对黄瓜霜霉病菌孢子囊萌发具有较好的抑制效果,且对孢子囊芽管发育具有一定的致畸效果。

Purify air, removes in the unusual smell and the fetid odor, absorb and clean air dust, the pollen, tiny bacterium sporangium, the germ and so on.

净化空气、去除烟味及臭味、吸收、清洁空气中的尘埃、花粉、微菌芽孢、病菌等。

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She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

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