查询词典 germ cells

An experiment was performed to compare effect of different cell media on embryonic germ cells derived from primordial germ ridge of Kunming albinotic strain mouse.

以昆明白品系小鼠胎儿生殖嵴为材料，以不同的培养液分离培养胚胎生殖细胞，发现小鼠胎儿肝细胞条件培养液的效果好于未添加白血病抑制因子的基础培养液，而差于添加了1 000 IU/mL LIF的基础培养液。

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要：通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产3 wk龄子代鼠进行PCR初选，再通过Southern杂交进一步确证；利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

AIM:Studies on totipotential stem cells using primordial germ cells have the same application prospect as embryonic stem cells, especially for the animals difficult to isolate embryonic stem cells from blastula.

目的：应用原始生殖细胞进行全能干细胞的研究具有与胚胎干细胞同样广阔的前景，尤其适合于难以从囊胚内细胞团中分离培养胚胎干细胞的动物。

Embryonic germ cell lines were isolated and cloned from gonad,gonadal ridge,analogue or the surrounding tissues of 9.5～13.5 days postcoitum mouse fetuses.Primordial germ cells were co-cultured with mouse embryonic fibroblasts obtained from the same conceptus.

自交配后9.5～13.5 d小鼠胎儿生殖嵴/腺或其类似物及周围组织，采用与其同源胎儿成纤维细胞共培养的方式分离克隆到具小鼠ES细胞诸多特征的胚胎生殖细胞系。

In order to investigate the changes of HSF2 mRNA expression and its role in germ cell apoptosis induced by heat stress or a high of testosterone undecanoate,we established the operation induced unilateral cryptorchid and TU injected monkey models,and examined in situ analysis of testicular cell DNA fragmentation and the expre ssion level of HSF2 mRNA in germ cells after treatment.

为了探讨HSF2mRNA在热应激和超生理剂量睾酮诱导恒河猴生精细胞凋亡中的表达变化，我们建立了手术诱导单侧隐睾和注射大剂量 11酸睾酮恒河猴动物模型，应用 3′末端标记分析和原位杂交方法，检测睾丸细胞的凋亡信号和HSF2的表达变化。

Germ potential is established after conception in a totipotent zygote and retained subsequently during development in blastomers of morula , cells form the inner cell mass of blastocyst , epiblast and population of primordial germ cells .

种子潜能的确定是全能的受精卵形成之后，并且随后在桑葚胚，胚泡内层细胞，外胚层和原生殖细胞的发育过程中保留。

Spontaneous germ cell death is very important for the removal of germ cells and control of the quantity and quality of sperm. But excessive germ cell death will lead to diminish spermatogenic function and decrease the quality and quantity of sperm. Excessive germ cell death triggered by various regulatory stimuli, including deprivation of gonadotropins and intratesticular testosterone or by estradiol treatment in rats via apoptosis.

生精细胞凋亡是哺乳动物精子发生过程中的一个显著特点，其生理性凋亡对于去除受损的生精细胞，控制细胞数量和保证精子的质量具有重要意义，但生精细胞的过度凋亡可引起生精功能低下，使精子的数量和质量降低。

ABSTRACT Objective To discuss the clinical pathologic features and differential diagnosis of mixed germ cell sex cord stromal tumorwith malignant variant of germ cell tumor Methods The clinical pathologic datum and immunophenotype were studied in the case of ovary MGCSCST with malignant variance of germ cell tumor Results The patient's genital anatomy and female phenotype were normal,karotypes was 46xx with uterogestation The tumor tissue consisted of mixed germ cells and sex cord stromal cells with evident malignancy of mixed germ cells There was no atypical structure of gonadoblastoma in tumor tissue Conclusion MGCSCSST has complex morphosis,thus,to prevent misdiagnosis,more samples should be collected and more slices be cut The patient may have prognosis malo when there are high malignant variants of mixed germ cells in tumor tissue

目的 探讨混合性生殖细胞性索间质肿瘤(mixed germ cell sex cord stromal tumor，MGCSCST)伴恶性生殖细胞肿瘤变异型的临床病理特点及鉴别诊断。方法卵巢MGCSCST伴恶性生殖细胞肿瘤变异型的病例进行临床病理特征和免疫表型分析。结果患者生殖器的解剖结构和女性表型正常，染色体组型是46xx，足月妊娠。肿瘤组织由混合性生殖细胞和性索间质肿瘤构成，混合性生殖细胞有显著的恶性特征。瘤组织中没有典型的性腺母细胞瘤结构。结论 MGCSCST有复杂的形态结构，因此更多的取材和切片可以防止误诊，当肿瘤组织中的混合性生殖细胞有高度恶性变异时，患者预后不良。卵巢肿瘤；生殖细胞性索间质肿瘤；免疫组织化学

Stage I of both male and female stone flounder appears only once in all its life, and the germ cells are comprised by spermatogonia or oogonia. The gonad from Mar to Aug keeps at stage II. The gonad index of testis at this stage is 0.037%, and the amount of spermatogonia is increased quickly. There is some linear germ plasm in the cytoplasm of spermatogina. In ovary at this stage it is mostly composed by oocyte of phase 2 which the character is the appearance of yolk nucleus, and no zona radiate in membrane. The mean GI of ovary is 1.95%. From Sep to Oct gonad is at stage III which testis is composed by lots of spermatogina and few spermatocytes, and the mean GI of testis at this stage is 0.086%. In ovary the ooctyes at phase 3 are in dominate position, the yolk nucleus disappear. And the GI of this stage is 3.35%. Both testis and ovary are at stage IV in Nov. hi testis the germ cells are in spermiogenesis, and the mean GI is 0.93%. hi ovary the oocytes are mostly at phase 4, which are filled in the cytoplasm with vitellin granule, and the zona radiate in membrane begins to formation. Nucleus moves to one side of the oocyte gradually. The mean GI of ovary at this stage is 9.37%.

在每年的3月-8月期间性腺处于Ⅱ期，此期精巢中精原细胞明显增多，胞质局部可见有线状的生殖质存在，平均成熟系数为0.037％；卵巢中以2时相卵母细胞为主，可见细胞质中出现强嗜碱性的卵黄核，细胞外由一层滤泡细胞包围，但尚无放射带，平均成熟系数为1.95％。9月-10月期间性腺处于Ⅲ期，此期精巢中仍有大量精原细胞，同时可见部分精母细胞，平均成熟系数为0.086％；卵巢中以3时石鲜孟加限加$玩印面n洲匆s性腺发生、分化及发育的周年变化相卵母细胞为主，细胞质中的卵黄核己消失，平均成熟系数为3.35%。11月性腺处于IV期，此期精巢内精细胞正处于不同的形成过程中，平均成熟系数为0.93%；卵巢中以4时相卵母细胞为主，胞质中充满染成桔红色的卵黄颗粒。

The Piwi clade is found in all animals examined so far, and its presence is tightly correlated with the emergence of specialized germ cells. Most animals separate germline and somatic cells early in development and restrict Piwi expression specifically to germ cells.

Piwi蛋白迄今为止被发现存在于所有动物中，它的存在与专门的生殖细胞紧密相关，大多数动物在发育的早期开始了不同组织和体细胞的分离和分化，并且对于生殖细胞的Piwi蛋白的表达进行特别的限制。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失