查询词典 genomic

Methods: 70 patients with COPD and 70 healthy volunteers were enrolled in this study. Limosis peripheral blood was taken and whole blood corpuscle genomic DNA was extracted. The genetic polymorphism of SOD1-3 genes was determined by polymerase chain reaction and restriction fragment length polymorphism.

收集70例COPD患者和70例健康人空腹外周血，提取全血细胞DNA，采用聚合酶链式反应－限制性片段长度多态性法检测两组SOD1-3基因的多态性。

ABSTRACT In order to screen chicken heart type fatty acid-binding protein gene genomic DNA library was constructed in λ phage. Four positive clones containing chicken H-FABP gene were obtained.

本研究通过构建北京油鸡基因组DNA噬菌体文库，筛选含鸡心脏型脂肪酸结合蛋白基因的重组子，克隆出4株含鸡H-FABP基因片段的重组噬菌体。

In this study,by PCR amplification with the genomic DNA of three mapping parents,940 SSRs primers were tested for analysis their versatility and polymorphism in melon,cucumber and watermelon.

利用940对SSR引物，对甜瓜、黄瓜和西瓜三套重组自交系群体的作图亲本DNA进行PCR扩增，分析SSR引物在不同瓜类作物间的通用性与多态性。

A relatively high proportion of bivalents were formed in all tri-genomic hybridsfrom different combinations.

通过加倍获得三个不同来源的六倍体(分别表示为六倍体Ⅰ，Ⅱ，Ⅲ)，在细胞学观察的基础上以确认为六倍体的植株为母本与诸葛菜杂交。

Morphologically and biologically, the triple-layered rotavirus particles are composed of three protein capsids, outer capsid includes VP4 and VP7, the middle is VP6, and the core is composed of VP2. VP1, VP3 and the 11 segments of double-stranded genomic RNA.

完整的轮状病毒颗粒直径约70nm，为正二十面体结构，由三层蛋白壳包绕，外壳为VP4及VP7蛋白，VP6蛋白构成病毒第二层蛋白壳，最内层为核壳，由结构蛋白VP1、VP2、VP3组成，核壳内包含有11个分节段的病毒双链RNA基因片段。

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

CP0645 and CP0782 gene were obtained by PCR using chlamydial genomic DNA as template.

以衣原体全基因组为模板，PCR获得衣原体 AR39 的 CP0645 和 CP0782 基因。

In this study, We typed the the gene of β〓-AR from genomic DNA of asthmatic patients and made the further analysis: The distributing condition of genetic polymorphisms at positions 16 and 27 in β〓 AR coding region and at position 19 in 5'Leader Cistron (5'LC) among southwest Chinese asthmatic population; The relationship between the each polymorphisms at the above three positions and the asthmatic phenotypes (FEV〓 or nocturnal asthma); The effect of each polymorphisms at the above three positions on bronchodilator responsiveness induced by β〓- agonists(initial bronchodilator response, pharmaco-dynamic); The relationship between the haplotypes of polymorphisms in the 5'LC or in positions 16, 27 of β〓AR coding block and asthmatic phenotypes or bronchodilator responsiveness induced by β〓-agonists.

实验通过对哮喘人群的β〓-AR基因进行分型，探讨：β〓-AR编码区16、27位点及5'前导肽(5'LeaderCistron，5'LC)19位点遗传多态性在中国西南地区哮喘人群中的分布情况；三个位点多态性与哮喘临床表现型的关系；三个位点多态性对β〓受体激动剂反应性(支气管舒张反应，舒张反应动力学)的影响；16、27位点及5'前导肽19位点遗传多态性单倍型与哮喘临床表现型及β〓受体激动剂反应性的关系。

Pig represents an evolutionary clade distinct from primates or rodents, and thus, provides considerable power in the analysis of human genomic sequences.

由于家猪在进化上和灵长类及啮齿类动物均存在差异，有关家猪基因组序列的研究将为解析人类基因组序列提供重要的信息。

Mate-paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR.

末端配对(meta-paired)体内鸟枪法基因组库被生成，并通过乳滴PCR 在微球上克隆扩增。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了