查询词典 genomic

Six pairs of primers with polymorphism among 36 accessions were used to amplify the genomic DNA.

利用分子标记SRAP技术，对36个甘薯品种进行了DNA多态性分析。

Restriction endonuclease digested genomic DNA was circularized by self-ligation, and complete gene of α subunit and β subunit was obtained by inverse PCR.

以环化的限制性内切酶消化的基因组片段为模板，用反向PCR的方法扩增、克隆并测定了α亚基和β亚基的完整基因序列。

Because different alleles of S-RNase are usually coded by the different amino acids in HV region, therefore,besides study on the genomic level, other approaches on the mRNA and protein level or cross-pollinated test are also important to confirm the S genotypes.

因此，除了在分子生物学的水平上进行研究外，其他方法如mRNA、蛋白质和杂交授粉等水平上的研究在确定S基因型上也同样重要。

To establish time-efficient and sensitive method for detection of the methylation of FMR1 and XIST genes,genomic DNA was deaminated by sodium bisulfite.

建立一种快速、灵敏的检测脆性X智障基因（fragile X mental retardation，FMR1）、X染色体失活基因（X chromosome inactivation，XIST）甲基化的方法。

Although adaptation decelerated sharply, genomic evolution was nearly constant for 20,000 generations.

适应性的发展加速极快，但基因组演化在20，000代几乎是不变的。

Although adaptation decelerated sharply, genomic evolution was nearly constant for 20,000 generations.

发现：尽管适应速率呈现出急速减速的现象，但是基因组的进化速率却在前20000代的时间里保持恒定。

Extracted DNA by CTAB, in proportion to the additions of PVP and β-mercap toethanol to the extraction buffer were capable of preventing browning from taking place in the extraction, Tris-Phenol (Stabilized with 0.1M Tris, pH 8.0)/Chloroform/isoamyl alcohol were used to remove protein and impurity in the second extraction, during crude DNA used to Isopropanol DNA and deterged by DNA detergent, without using RNase remove RNA, we detected the quality and concentration of DNA respectively for normal leaves and drought stress leaves by Agarose gel electrophoresis of genomic DNA and ultraviolet spectrometry photo.

用20%聚乙二醇(polyethylene glycol，PEG6000)模拟干旱，采取CTAB提取缓冲液，按一定比例加入PVP和β-巯基乙醇，用Tris平衡酚－氯仿－异戊醇除去蛋白质及杂质，在粗提时用异丙醇沉淀DNA，并用DNA洗液（10mmol/L醋酸铵，75%乙醇）洗涤DNA，在纯化时用乙醇沉淀DNA，且没有加入RNase去除RNA的方法，分别提取2个品种正常和干旱胁迫的苹果属叶片总DNA，并对提取的DNA进行琼脂糖凝胶电泳和紫外分光光度计检测。

When a given genomic DNA was hybridized to their own chromosomes, the signals were the strongest.

这说明它们间重复序列的变异是明显的。

Genomic DNA of maize was digested with restriction enzymes and hybridized with a mF2KP cDNA fragment.

应用不同的限制性内切酶消化玉米基因组DNA，以〓p标记的mF2KP cDNA片段作为探针进行Southern杂交分析。

DNA methylating inhibitor makes the transfer of the methyl group to adenine or cytosine impossible,thus the reaction of DNA methylation is inhibited,the genomic methylation level is decreased.

DNA甲基化抑制剂使甲基基团不能转移到腺嘌呤或胞嘧啶，导致DNA甲基化反应受阻，从而使基因组甲基化水平降低。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。