查询词典 genomic

An annotation is any feature that can be tied to genomic sequence, such as an exon, transcript, promoter, or transposable element.

注释是可以束缚在基因组序列上的任何特征，例如外显子、转录物、启动子或转座元件。

Methods The genomic DNA extracted from the 2 trematodes was amplified with random amplified polymorphic DNA and then was carried on agarose gel electrophoresis.

应用随机扩增多态性DNA（Random amplified polymorphic DNA，RAPD）技术对斯氏肺吸虫和华支睾吸虫的基因组多态DNA进行扩增，扩增产物经琼脂糖凝胶电泳后分析结果。

In a word, the CTAB method is most suitable for extracting genomic DNA from spores of Tremella fuciformis.

综合而言，CTAB法是五种方法中最为适合银耳芽孢基因组DNA提取的方法。

The reaction systems and cycling parameters of RAPD for Trichosanthes kirilowii Maxim were confirmed after the reduplicate experiments of genomic DNA abstraction and RAPD analysis. A total of 80 10-mer primers was tested.

通过叶片总DNA抽提、RAPD标记分析及反复的试验筛选，找到了一个适用于栝楼的RAPD反应体系和循环参数。

Viral replication is regulated at many levels, including the use of conserved genomic RNA structures. Most potential regulatory elements in viral RNA genomes are uncharacterized.

单链病毒RNA基因组内的二级结构已知具有几种功能和调控作用，但此前研究人员尚未对任何病毒的完整RNA进行过全面分析。

The genomic DNA of Unio douglasiae, collected from Suya Lake, Henan Province, was used as template DNA to optimize the RAPD-PCR reaction conditions including the concentration of DNA template, random primer, dNTPs, Mg2+ and Taq DNA polymerase and the annealing temperature for thermal cycle.

以河南宿鸭湖圆顶珠蚌的基因组DNA为模板，对淡水蚌类RAPD反应体系中的模板DNA浓度、随机引物浓度、dNTPs浓度、Mg~(2+)浓度、Taq DNA聚合酶浓度和和热循环参数中的退火温度进行了优化。

Bisulfite modification: A total of 1μg of genomic DNA, obtained from culture cells or tissues, was modified by sodium bisulfite to convert unmethylated cytosines to uracils.

2亚硫酸氢钠修饰取1μg上述DNA经亚硫酸氢钠处理，将非甲基化的胞嘧啶转变为尿嘧啶，而甲基化的胞嘧啶保持不变。

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白，主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运，并维持一定细胞内外的离子梯度，我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析，发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg，Fc。

The research found there is a high genomic similarity between the giant panda and the dog. It also supported the view of most scientists that the giant panda may be a subspecies of Ursidae.

研究结果表明，大熊猫基因组与狗的基因组最接近；数据分析结果还支持了大多数科学家所持的&大熊猫是熊科的一个亚种&的观点。

The universal primers of ITS1 and ITS4 for fungi were used in amplification of genomic rDNA sequences. A target fragment was obtained by ITS-PCR. Then, the fragment is cloned into the vector of pMD18-T before sequencing. The result of sequencing showed that the fragment is 692 bp in length. The results of Blast in Genbank and the analysis of fungal rDNA systemic development show that the relation between this fragment to the fungi of Sporisorium is closest, while the relation between it to the fungi of Ustilago is much far. It suggests that the name of Ustilago scitaminea for sugarcane smut isn't consistent to the result of rDNA systemic development analysis. It still needs more experiments to support this hypothesis.

用真菌rDNA序列分析的通用引物ITS1和ITS4，通过ITS-PCR得到目的片段，克隆在pMD18-T载体上后进行测序，结果显示该片段的长度为692 bp，与Genebank中已报道的真菌序列进行Blast和系统发育树分析，表明所获得的序列与Sporisorium属真菌具有更高的亲缘关系，而与Ustilago属真菌的亲缘关系较远，这与一直沿用的甘蔗黑穗病菌的命名似乎并不吻合，有待进行更多的研究以证实。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了