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This report identified S-RNase genes of sweet cherry (Prunus avium L.), presented the sequences of S genes by using a pair of specific primers PruC2 and PruC4R based on the conserved regions C2 and RC4 of Rosaceous S-RNase genes and researched the S gene specific productsfrom the genomic DNAs of different cultivars in which most of the S genotypese were unknown.

根据蔷薇科S-RNase基因高度保守区C2 和RC4区设计一对特异引物PruC2 和PruC4R，对甜樱桃品种的基因组DNA进行S基因特异PCR扩增。

In silico cloning is developing with the project of genomic and EST. It is a new method of gene-cloning through bioinformatics.

电子克隆是随着基因组计划和EST计划实施而发展起来的利用生物信息学手段进行基因克隆的新方法。

The specifical concentration of cholesterol could decrease the VECs genomic DNA methylation level.

一定浓度的胆固醇能降低血管内皮细胞中DNA甲基化水平。

The genomic DNA of 31 accessions of genus Tagetes was amplified with 11 primers, the bands produced by each primer ranged from 5 to 11, and 6.8 bands were obtained on average, the polymorphic loci ranged from 40%-100%.

用11个引物对31份万寿菊属材料进行PCR扩增，每个引物扩增的ISSR条带数在5-11条，平均每条引物能扩增出6.8条带，多态位点百分率为40%-100%。

In PCR detection systems, the DNA of recombinant plamid of TCK was used for safely positive reference and genomic DNA of the healthy wheat and rye was used for negative reference.

选择重组质粒作为检测中的阳性对照，健康小麦和健康黑麦基因组DNA作为阴性对照。

Genomic DNA of two fungi Thamnidium elegans and Umbelopsis isabellina were extracted with an amended Cetyltrimethyl Ammonium Bromide method.

以丝状真菌雅致枝霉和深黄伞形霉为材料，使用优化的CTAB法提取基因组DNA。

On other hand, specific fragments containing thermostable DNA polymerase gene were amplified from the genomic DNA of some strains of thermophilic bacteria isolated from Yunnan hot springs, and recombinant plasmids were constructed.

同时，我们还利用特异性的热稳定DNA聚合酶基因的扩增引物，从生长在云南温泉的嗜热菌总DNA中筛选出了能被此类引物扩增的特异性片段，并构建了相应的重组质粒。

Torenia fournieri genomic DNA was digested with DraI、EcoRV、PvuII、SmaI respectively. A special adaptor was ligated to the ends of the digested DNA fragments as a template for adaptor PCR. With the adaptor and TfPLC1 gene-specific primers, bands of 798bp and 813bp upstream of TfPLC1 were obtained successively.

应用衔接头PCR技术，以蓝猪耳全基因组DNA分别经DraI、EcoRV、PvuII、SmaI消化后与衔接头连接产物为模板，用衔接头引物和TfPLC1基因的特异引物经过多轮的巢式PCR，先后克隆到两个大小为798bp、813bp的TfPLC1基因上游序列；经测序、blastn比较分析和拼接得到一个蓝猪耳TfPLC1基因的启动子序列，共1432bp。

The Laccase gene of Trametes versicolor's genomic DNA amplified by PCR using a set of primers that we designed in this study. The amplified fragment was cloned into the vector pUCM-T.

本研究以PCR扩增的方法，从变色栓菌的基因组DNA中扩增出了一预期大小的DNA片断，并将其克隆到了pUCM-T载体上。

MOTIVATION: In order to facilitate a systematic study of the promoters and transcriptionally regulatory cis-elements of the yeast Saccharomyces cerevisiae on a genomic scale, we have developed a comprehensive yeast-specific promoter database, SCPD.

动机：为了促进在基因组范围上对酵母中启动子和转录调控顺式调控元件的系统性研究，我们开发了一个全面的酵母特异的启动子数据库----SCPD。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了