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RT-PCR detection of SYT-SSX mRNA can be used in formalin-fixed, paraffin-embedded tissues as a sensitive and specific technique in the diagnosis and differential diagnosis of synovial sarcoma. 2. The SYT-SSX fusion type is related to histological type and cell proliferative activity of synovial sarcoma. 3. Some characteristic sequence motifs flank genomic breakpoints of chromosomal translocation of synovial sarcoma, including Alu sequences, Translin recognition sequences, topoisomerase Ⅱ recognition sites and palindromic sequences, which may be associated with chromosomal translocation of synovial sarcoma.

1、RT-PCR技术检测SYT-SSX mRNA表达可用于福尔马林固定、石蜡包埋组织，敏感性和特异性高，可用于滑膜肉瘤诊断和鉴别诊断。2、SYT-SSX融合基因类型与滑膜肉瘤组织学分型及细胞增殖活性相关。3、滑膜肉瘤染色体易位断裂点旁存在一些特征性序列，包括Alu序列、易位素识别序列、拓扑异构酶Ⅱ识别位点、回文序列等，可能与其染色体易位发生机制有关。

There were some characteristic sequences flanking the genomic breakpoints of SYT, SSX1 and SSX2. These sequences included Alu sequences, Translin recognition sequences, topoisomerase Ⅱ recognition sites and palindromic sequences. These sequences are speculative to be associated with chromosomal translocation of synovial sarcoma.

在SYT，SSX1和SSX2基因组断裂点旁存在一些特征性序列，包括Alu序列、易位素识别序列、拓扑异构酶Ⅱ识别位点、回文序列等，可能与滑膜肉瘤染色体易位有关。

Three varieties of Rutaceae plants including C. grandis Osbeck, Acronychia pedunculata Miq., Z. avicennae DC were used as material for study. The ISSR reaction system included the genomic DNA extraction, amplification, cycle time, primers screening.

方法］以象头山芸香科植物柚、山油柑、3个品种为研究对象，从DNA提取、退火温度、循环次数、引物筛选等方面研究ISSR反应及优化条件。

The genomic DNAs were extracted from Phaseolus vulgaris, Vigna sesquipedalis and Glycine max with CTAB method.

以改良的CTAB法，分别从菜豆、豇豆和大豆叶片中提取了基因组DNA。

Molecular phylogenic analysis in post-genomic era.

后基因组时代的系统演化分析

For better understanding of the phylogenic diversity and evolution of FSH p molecule, we have isolated and sequenced the genomic sequence encoding Chinese swamp buffalo FSH p precursor molecule by PCR.

为了更好地了解FSHβ基因的进化情况，我们利用PCR方法对中国沼泽型水牛的该基因的基因组序列进行了克隆测序。

It was reported that the pHZ1392 isolated from a genomic cosmid library of 10-22 might contain the complete gene cluster for 5102-Ⅱ bio-synthesis, which can inhibit some pathogenetic fungi, such as Cochliobolus heterostrophus and Piricularia oryzae, etc.

在过去的研究中曾报道10-22基因文库中的一个粘粒-pHZ1392可能含有完整的5102-Ⅱ抗生素合成基因簇，如果以pHZ1392转化变铅青链霉菌ZX64，可以使该宿主菌获得对玉米小斑病菌的抗性。

2Some specific nucleoside acid bands were amplified from genomic DNA of male and female plants with RAPD technique, indicating that some gene marks related to sexes of Pistacia Vera were found. RAPD signal is an reliable, accurate identifying technique on sexidentification of Pistacia Vera.

2利用RAPD手段对阿月浑子雌雄株基因组DNA扩增后找出性别之间差异性的核苷酸片段，即找到部分与阿月浑子性别相关的基因标记，从而快速而有效地鉴别雌雄株，差异显著。

As a polygenetic inheritance tumor, NPC has the characteristic of obvious tendency of familial aggregation, possesses genomic instability, and is vulnerable to physical, chemical and biological carcinogenic factors.

鼻咽癌是我国南方常见恶性肿瘤，具有明显的家族聚集倾向，存在基因组不稳定性，易受理化、生物等环境因素的影响，是多基因遗传性肿瘤。

The genomic DNA were extracted from the leaves of Polygonatum roseum in Xinjiang and the primers were designed according to conservative sequences of Polygonatum lectins gene.

用百合科黄精属植物凝集素基因的保守序列为引物，从新疆黄精的叶中克隆出黄精凝集素的全长cDNA。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了