查询词典 genomic

Objective:To construct a genomic library of Proteus mirabilis and identify signature sequence.

目的：构建用于筛选奇异变形菌的特异序列的基因组文库，并从中筛选变形杆菌特异的标识序列。

Using the primers from SRY gene-HMG Box for PCR amplification in genomic DNA of Muntiacus reevesi cell strains, a 220bp fragment was obtained in the male but not in the female.

应用人的性别决定基因SRY (Sex-determining Region Y gene， SPY)中HMG框内的一对引物，对小麂细胞株的基因组DNA进行PCR扩增，得到雄性小麂细胞的220bp扩增产物，而在雌性小麂细胞中未发现扩增产物。

The Germplasm Bank of Wild Species is a national key project of scientific facilitation in China. The wild germplasm resources, including seeds, micro-propagated samples, genomic DNA/cDNA libraries, and mycological spores/bodies, are the basis for human sustainability.

中国西南野生生物种质资源库是国家大科学工程，其保藏的野生种质资源是人类社会可持续发展所依赖的战略资源。

Now, I mainly engaged in myxobacterial genomic and bioinformatics analysis work. Myxobacteria is a single-cell microorganisms, but they act as multi-cell behavior (such as feeding, social movement, cells congregating and fruiting body formation, and so on) and own a wealth of secondary metabolites Product, those remarkable characteristics make myxobacteria important in the research fields of prokaryotes cell differentiation and development, biological evolution, as well as the drug development and research.

现主要从事粘球菌基因组与生物信息学分析方面的研究工作：粘细菌是单细胞微生物，然而却具有多细胞行为（如摄食、社会性运动、细胞聚集和子实体形成等）和丰富的次级代谢产物，这些行为特征使粘细菌在原核生物的细胞分化发育、生物进化以及药物开发研究中占有重要地位。

The resulting strain, designated YES2MTM1, was transformed with a yeast genomic library. Transformants lost the plasmid overexpressing MTM1 after 5-FOA treatment. Yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and overexpression of some DNA fragments were picked up and candidate suppressor genes were identified. Overexpression of five genes were identified to be able to rescue the growth defect on nonfermentable carbon source. The study will provide reference for MTM1 gene function and screening for suppressor of genes whose deletion result in irreversible damage.

为了避免MTM1缺失造成的不可逆损伤，在野生型酵母中先转入带有MTM1 基因的质粒，再敲除染色体上的MTM1 基因，随后转入基因组文库，再利用药物5-氟乳清酸(5-FOA)迫使细胞丢失表达MTM1基因的外源质粒，再筛选能在非发酵培养基上生长的转化子，通过这种方法筛选发现，POR2等5个基因的过表达可以挽救MTM1 基因缺失造成的非发酵培养基上的生长缺陷，为深入了解MTM1基因的功能提供了线索，对筛选其他造成不可逆损伤的突变基因的抑制基因提供了一条可行的研究思路。

Results They could find the influence of pseudogene through performing genomic DNA and nonreversed transcribed RNA controls.

结果 通过设立分别以基因组DNA和未经逆转录的RNA为模板的对照可观察到CK19假基因的干扰现象。

The genomic DNA of Nosema bombycis was isolated from it's spores, using two methods, ie, Alkaline-Germination and Liquid-nitrogen.

分别用碱发芽法及液氮法抽提了家蚕微孢子虫基因组DNA，其中碱发芽法抽提的DNA质量及数量上好于液氮法抽提的DNA。

Based on the genomic DNA extracted from Ochotona curzoniae, the effects of the main reaction system elements such as Mg(superscript 2+), dNTPs, primer, template DNA, Taq DNA polymerase and annealing temperature were presented by single factor experiment.

方法］以高原鼠兔基因组DNA为模板，通过单因素试验，对体系中的模板浓度、Mg(上标 2+)浓度、dNTPs浓度、Taq酶用量、引物用量、退火温度进行探讨。

Ocimum Biosolutions is a leading integrated genomics company providing GLP-compliant microarray services, comprehensive genomic reference databases, life science lab information management solutions and essential research consumables.

Ocimum Biosolutions 是一个领先的综合基因组学公司，提供GLP规范兼容的芯片服务，全面的基因组参考数据库，生命科学实验室信息管理解决方案和基本研究消耗品。

Methods : OMP2b protein gene fragment was amplified from genomic DNA of Brucella ovis in XinJiang through PCR.

采用PCR扩增技术，从新疆绵羊种布鲁氏菌基因组DNA中扩增出OMP2b基因片段，将该片段克隆于原核表达载体PE-28a，构建成重组质粒，IPTG诱导表达，SDS-PAGE检测有无蛋白的表达。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了