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the modified ctab method was suitable for the extraction of genomic dna from lonicera japonicathunb.dry leaves.

改良ctab法适合忍冬干叶基因组dna的提取。

objective:to extracthigh quality genomic dna from lonicera japonicathunb.dry buds with silica gel.

摘 要:目的:从变色硅胶干燥的忍冬叶片中提取高质量的基因组dna。

Therefore the nonrandom distribution of 3′ end sequences of effectual primers in tomato RAPD reflected the sequence-specific structure may occur in tomato genome at flank of polymorphic regions. It is probably an attribute of tomato genomic sequence and is common in Lycopersicon.

RAPD引物3′端序列的非随机分布,反映了番茄基因组内的多态性区段侧翼具有序列的特异性,这种特异性结构应是番茄基因组序列的一种属性,这种属性为番茄属基因组所共有。

The results showed as follow:(1) The method of improved CTABⅠequal to extract Magnoliaceae genomic DNA was developed, because the extracted DNA by the method was suitable for RAPD and ISSR analysis.(2) Single factor design combing uniform design was used to obtain optimum scheme on reaction system for RAPD and ISSR of Magnoliaceae.

研究结果如下:(1)建立了适合于木兰科6属20种植物DNA提取的改良CTAB法Ⅰ,用该法提取的DNA能满足RAPD和ISSR分析的要求;(2)采用单因素结合均匀试验设计优化得到适合于木兰科6属20种植物RAPD和ISSR分析的PCR体系。

Based on using those specific PCR primers, a unique 190 bp hand was obtained by PCR amplification from genomic DNA of Venturia inaequalis of apple and it can be accurately distinguished from patbogens of Alteraria mali, Marssonia mali and Podosphaera leucotricha, the common apple leaf spot diseases.

利用这对引物能从苹果黑星病菌基因组DNA中扩增出一条分子量为190bp的特异性条带,准确地把苹果斑点落叶病、苹果褐斑病和苹果白粉病等常发性苹果叶斑病害区分开。

The improved CTAB method was applied to extracted genomic DNA in the leaves of 7 species of Michelia. Using the method of single factor in the experiment, the effects of different concentration of MgCl2, dNTP, template DNA, random primers and Taq DNA polymerase on RAPD reaction were studied.

方法]采用改进的CTAB法提取木兰科含笑属7种植物叶总DNA,进行RAPD分析,分别研究了模板DNA、引物、dNTP、Mg(上标 2+)和TaqDNA聚合酶用量对RAPD反应的影响。

Forty microsatellite loci were selected to amplify the genomic DNA of cultured stock of largemouth bass Micropterus salmoides L.

本研究在人工养殖的大口黑鲈(Micropterus salmoides L。

Object to the increasing numbers of transposable elements in protozoa genome, this review focus on the types and genomic distributions of transposable elements in newly completed genome of protozoa parasite, mainly including Trypanosoma, Leishmania, microsporidia, Amoebozoa.

针对近年来原生动物全基因组序列中大量发现的转座元件,文章着重比较了转座元件在锥虫、利什曼虫、微孢子虫、变形虫和滴虫基因组序列中的存在种类、分布特征及其功能意义。

By the comparision of the divergence of transposable elements in microspordian genome, it was hypothesed that at least one loss event of transposable element had occurred during microsporidian genomic evolution.

根据不同种微孢子虫基因组之间转座元件的差异,推测在微孢子虫基因组进化过程中,至少经历了一次转座元件的丢失事件。

Determine the virus titer by microtitration and plaque formation test and the virus proliferation by IFA, and perform genomic stability test.

用微量滴定法与蚀斑法平行检测病毒滴度,用免疫荧光法检测病毒的增殖情况,最后进行病毒基因组稳定性检测。

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