查询词典 genomic

Compared with fresh samples, dried with silica gel, the genomic DNA was extracted from Castanea henryi leaves, which preserved for 10 days, 2 months, 4 months, 6 months, 8 months, 10 months, 11 months, 12 months, 13 months, 14 months, 15 months, 16 months, 17 months, 18 months, by improved CTAB methods.

以野生锥栗幼叶为对照，采用硅胶干燥法，设置不同保存时间（10 d，2、4、6、8、10、11、12、13、14、15、16、17、18个月），对保存不同时间的锥栗叶样抽提基因组总DNA，利用紫外分光光度计及琼脂糖凝胶电泳检测提取DNA的浓度、纯度、得率及质量等指标，评价不同保存时间对基因组DNA的影响。

The coding sequence of CBD was amplified from the genomic DNA of Cellulomonas fimi by PCR using specific primers and fused with scFv. However, the product is insoluble despite of optimizing the expression conditions.

设计特异引物，我们从Cellulomonas fimi基因组DNA上克隆出了一个纤维素结合域序列，并将其与抗膀胱癌单链抗体构成融合表达载体，但表达产物不可溶。

In this study, partial fragments of potassium ion channel gene were amplified using the genomic DNA of muntjak, reevesi, crinifrons, and Elaphodus cephalophus.

运用PCR扩增、T-A克隆、测序等技术，获得黑麂，小麂，赤麂和毛冠鹿4种麂亚科动物基因组DNA的钾离子通道部分序列。

Objective To investigate the genomic DNA extraction methods of Cephalosporium acremonium which produce Cephalosporin C.

当提取液pH值为 9 0～10 0、EDTA浓度为 12 0mmol·L-1时，所得的顶头孢霉染色体DNA的质量和数量均较理想，每克顶头孢霉菌丝体能得到 96 μg的染色体DNA。

Partial sequences of chalcone synthase gene exon 2 were amplified by PCR method from genomic DNA of 10 species of the basal eudicots. After cloning and sequencing, 26 seqences were obtained.

用PCR方法从目前极少被报道的真双子叶基部类群10种植物的总DNA中扩增出CHS基因外显子2的部分序列进行分析，经克隆后测序，共得到26个不同的片段。

The purpose of the work is to investigate the frequencies and polymorphisms of HIV-1 resistant CCR5delta32,CCR2b-64I,SDF1-3′A alleles in Chinese Dai and Chingpaw populations.Whole blood samples from 101 Dai subjects and 113 Chingpaw were collected randomly and their genomic DNA were extracted with QIAgen Blood Kits.Allelic frequencies were identified by PCR-RFLP analysis.Allelic polymorphisms in Dai population or Chingpaw population and both sexes in the samples were analyzed by χ2 test.

为了调查HIV-1感染相关的等位基因CCR5△32、CCR2b-64I、SDF1-3′A在我国云南省德宏州傣族景颇族人群中的频率和多态性分布，此课题以101例傣族和113例景颇族人群为研究对象，应用PCR、PCR-RFLP(聚合酶链反应-限制性片段长度多态性)分析方法进行检测，计算突变基因频率；并对其群体分布、性别分布进行统计学分析。

Objective To analyze the polymorphism of the genomic DNA extracted from Paragonimus skrjabini and of that from Clonorchis sinensis and explore the similarity of their genes.

目的 比较分析斯氏肺吸虫和华支睾吸虫基因组多态DNA的多态性和探讨两吸虫间的相似基因。

It is proposed that coadministration of carbachol could prevent apoptosis and related alterations in gene expressions induced by sodium selenite just as the carbachol did in the case when apoptosis and related changes in genomic expression were induced by β-amyloid peptide. The facts suggest that apoptosis, at least that induced by two agents tested by us, might be initiated through a similar pathway.

这些结果表明，亚硒酸钠能在较早的时间诱导培养的大脑皮质神经元凋亡，且具有明显的剂量和时间依赖关系；凋亡的引起可能是以亚硒酸钠影响某些特殊基因的表达为基础，其中，c-fos、bax、P53、和bcl-表达的变化主要与细胞凋亡的早期事件有关，而AChE基因表达的增强同凋亡发展的整个过程密切相关。

To identify whether our previous founding were exist in the series of our newly established cell cultures with the same genetic background and different metastatic potentials, and to make clear what are congenerous and various traits in the chromosomal or DMA sequence levels between these cells, in this study, a combination of conventional G-banding, comparative genomic hybridization, multiplex fluorescence in situ hybridization and arm or locus-specific fluorescence in situ hybridization were used to comprehensively characterize molecular cytogenetics aberrations of the above cell cultures.

为查明我所新建立的这些具有同一遗传背景而又在肝癌的转移潜能上存在明显差异的细胞是否也具有8p的缺失，并弄清它们在其它染色体及其区段的DNA水平上有着怎样的相似和不同，这些相似与不同又有着怎样的意义，本课题联合应用G显带技术、比较基因组杂交技术、多重荧光原位杂交技术和位点或臂特异荧光原位杂交技术，对上述细胞进行细胞及分子遗传学研究，以期了解其分子细胞遗传学异常的特征、找出转移相关的遗传学标记，为探讨肝癌转移机制提供材料。

At present, fourteen melon genetic maps have been constructed, many molecular markers linked to important traits and quantitative trait locis are found and some has been located on the maps. The research of compatative genomic maps on cucurbitaceous crops has begun.

目前己构建有14张甜瓜遗传图谱，多个重要性状的分子标记及数量性状位点己找到并定位在图中，并开始在葫芦科作物之间进行比较基因组图谱方面的研究。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。