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With the advent of post-genomic era, identification of protein-protein interaction has become another hot spot of protein research and promoted the invention, development and complement of relative techniques.

随着后基因组时代的到来，阐明蛋白质间相互作用关系成为蛋白质研究的又一热点，促进了相关技术的不断产生、发展和完善。

To resole this discrepancy, we performed single-cell PCR analysis of microdissected eGFP-positie hepatocyte-like cells and found that they contained mostly mouse and little human genomic material.

为了分析这种偏差，我们对显微解剖的类似肝细胞的eGFP阳性细胞进行单细胞PCR分析，发现他们包含了绝大多数鼠类和极少部分人类基因组物质。

Through the comparisons, it was observed that there were extensive conserved relationships of maize QTL affected plant height, row number, and kernels per row with rice QTL affected plant height, tillers per plant, and grains per panicle respectively. 16 of 45 QTL affecting five different maize traits were conserved compared with 12 of 38 QTL affecting five different rice traits, which provided some useful information for locating, isolating and cloning maize QTL by using the rice genomic data.

研究结果表明：在玉米和水稻共线性的染色体区段，控制玉米株高、行数和行粒数的QTL与控制水稻株高、单株有效穗和每穗实粒数的QTL存在广泛的对应关系；在已定位的影响玉米株高等5个性状的45个QTL中，有16个与水稻"汕优63"群体中5个相同或相似性状所定位的38个QTL中的12个具有共线性关系。

PCRs were performed on genomic DNAs of four pear cultivars Sand pear, Huangguan pear, Xueli pear, Yali pear using primers derived from sequences of previous published PPO. The target bands were recovered and purified. The fragments were ligated with the plasmid of pGEM-T Vector by the method of direct T-A cloning.

以4种不同来源的梨基因组DNA为模板，根据已经发表的多酚氧化酶基因序列设计引物，利用PCR技术，克隆到了鸭梨、雪花梨、砂梨、黄冠梨约1.8 kb的完整多酚氧化酶基因，将纯化后的扩增产物克隆到质粒pGEM-T vec-tor中，转化DH-5α菌，挑选阳性克隆进行测序。

They include the variation of the degradement velocity of different sample, the inhibition product of PCR, the amount of DNA extraction, quality of DNA and the heteroplasmy of some tissue. Correct sequencing, comparison of the result of forward and reverse primers sequencing should be done to rule out wrong signals, and prevent contamination. In addition to gene sequencing, low grade heteroplasmy could be dealt by Denaturing high performance liquid chromatography. Multiplex Ligation-dependent Probe Amplification. The analysis of mitochondrial HV Ⅰ, HV Ⅱ sequencing is able to identify the maternal sanguinity, then it could assist in the human identification. It also complements the genomic STR system in distribution of population, the origin of human, and analysis for fossil.

分析粒线体DNA时必须考虑各类检体腐败DNA降解速度之不同、PCR的抑制物质、DNA的萃取量及品质、特定组织的异质现象、萤光序列分析图及正、反向引子定序结果比对、排除杂讯干扰、防治污染等，除以基因定序外，低程度的异质现象也可用Denaturing high performance liquid chromatography、Multiplex Ligation-dependent Probe Amplification等方法分析，粒线体HV Ⅰ、HV Ⅱ碱基序列的分析可鉴别一个人的母系血缘关系、辅助人类身分鉴定，对於族群分布、人类起源探讨、远古检体的分析可作为辅助STR系统的利器。

In order to study function and molecular polymorphism of classⅡB gene in fish, we have isolated cDNAs encoding classⅡB from spleen cDNA library of red sea bream by using EST sequencing, and examined genomic organization, molecular polymorphism and expression of red sea bream classⅡB gene.

在所测序的11个不同的阳性克隆中，有4个不同的cDNA序列被鉴定出。另外，利用rsbMHC2b3'UTRN和rsbMHC2b3'UTRC为引物，扩增同一真鲷个体的3'UTR，有12个阳性克隆被测序，测序结果中有2个不同的序列被鉴定出，表明MHCⅡB基因至少存在两种不同的位点。

Structural analysis reveals that NAG73 has an intronless genomic sequence, which is homologous with the fourth exon and 3′UTR of Homo sapiens Growth Hormone Secretagogue Precursor Gene.

结构分析显示NAG73的基因序列无内含子，无典型的启动子序列， poly A尾。

By using bulk segregant analysis method, resistant and susceptible DNA bulks were constructed with 10 resistant and 10 susceptible inbred lines genomic DNA, respectively.

采用集团混合分组分析法，分别以10个抗病和10个感病玉米自交系基因组DNA构建抗病基因池和感病基因池。

According to the published genomic sequence of TMV ,five sets of PCR primers were designed and five nucleotide segments were amplified by RT-PCR from the total RNA of the TMV infected Rehmannia glutinosa leaves.

根据已发表的TMV基因组的核苷酸序列设计5对简并引物，以感染TMV的地黄叶片总RNA为模板，经RT-PCR扩增获得TMV-RH的5个核苷酸片段的cDNA。

The glycoprotein-encoding ORF117 gene was cloned by PCR from genomic DNA of sheep pox virus isolated in Jingtai, Gansu Province.

以甘肃景泰疑似羊痘患病绵羊的皮肤组织中提取的总DNA 为模板，经PCR 扩增获得SP 病毒 ORF117 基因。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了