查询词典 genomic

The relationship between rates of genomic evolution and organismal adaptation remains uncertain, despite considerable interest.

基因的进化与生物体适应速率之间的关系，很多人都很关注，但是仍然不很确定。

Recombinant adenovirus particles were produced after 293 cells transfected with recombinant Ad genomic plasmid DNA digested with PacI, then they were further amplified in 293 cells.

纯化所得腺病毒滴度约为8×1012pfu/L，当MOI=100时，腺病毒感染HepG1细胞的效率＞75％，Western Blot证实在感染重组体腺病毒的细胞中有相应基因产物的表达。

To establish a method to differentiate Muscovy duck parvovirus from goose parvovirus, primers GPVF/R1 and MDPVF1/R1 were designed according to the genomic sequences of GPV and MDPV to amplify the virus culture and nucleotide of GPV, MDPV, duck plague virus, duck hepatitis virus, duck reovirus, canine parvovirus and feline panleucopenia virus.

根据番鸭细小病毒和鹅细小病毒基因组的非同源序列各设计了1对引物MDPVF1/R1和GPVF1/R1，建立了一种PCR方法，用该PCR方法分别对GPV、MDPV、鸭瘟病毒、鸭肝炎病毒、鸭呼肠孤病毒、犬细小病毒和猫泛白细胞减少症病毒的病毒培养物及其核酸进行扩增。

Lysis solutions from imported and domestic genomic DNA purification kits,and 2% Trition X-100 and 5%guanidinium isothiocyanate were added into the oocysts,followed by different combining forms of freeze-thawing,proteinase K and sonication.Real-time PCR was used to determine the copies of Cryptosporidium oocyst wall protein gene.HCT-8 cells were infected by C.andersoni oocysts.Real-time PCR was used to determine the enhancement effect of fetal calf serum,glucose,ascorbic acid,folic acid,calcium pantothenate and insulin while added into RPMI-1640 medium.

在隐孢子虫卵囊悬液中分别加入进口试剂盒和国产试剂盒的裂解液以及2%Triton X-100和5%异硫氰酸胍，辅以不同组合方式使用的冻融法、蛋白酶K法和声裂法裂解卵囊，通过Real-time PCR法测定裂解产物中隐孢子虫卵囊囊壁蛋白(Cryptosporidium oocyst wall protein，COWP)基因拷贝数。

This paper took the insect-resistant transgenic birch grown in greenhouse for four years as study materials. The genomic DNA and RNA of leaves were extracted and the integration and the expression characteristic of the extraneous genes in the insect-resistant transgenic birch were analysed by methods of multiplex PCR, reverse primer PCR, inverse PCR, RT-PCR, and insect-feeding experiment of the transgenic plants respectively.

本文以温室四年生转抗虫基因白桦为研究材料，提取叶片的基因组DNA和RNA，运用多重PCR，反向引物PCR，反向PCR和RT-PCR等方法，并结合转基因白桦的喂虫实验，分别对转基因白桦外源基因的整合特性和表达特性进行分析，所得结果如下： 1。

ASCL2 gene escapes genomic imprinting in skeletal muscle,fat, heart,liver,spleen,lung,kidney,stomach,small intestine,uterus,ovary and pituitary.(2) Both of the transcripts of NNAT gene is paternally expressed in skeletal muscle,fat,heart, liver,spleen,lung,kidney,stomach,small intestine,uterus,ovary and pituitary.(3) DIRAS3 gene is paternally expressed in skeletal muscle,fat,heart,liver,spleen,lung, kidney,stomach,small intestine,uterus,ovary and pituitary.(4) DLX5 gene is maternally expressed in skeletal muscle,fat,spleen,lung,stomach,small intestine,but escapes imprinting in heart,liver,kidney,uterus,ovary,testicle and pituitary.

1ASCL2基因在肌肉、脂肪、心、肝、脾、肺、肾、胃、小肠、子宫、卵巢和垂体中均逃脱印记；(2)NNAT基因两个转录本在肌肉、脂肪、心、肝、脾、肺、肾、胃、小肠、子宫、卵巢和垂体中均为母系印记：(3)DIRAS3基因在肌肉、脂肪、心、肝、脾、肺、肾、胃、小肠、子宫、卵巢和垂体中母系印记：(4)DLX5基因在骨骼肌、脂肪、肺、脾、胃和小肠中表达母本来源的等位基因，即表现为父系印记；而在心、肝、肾、子宫、卵巢、睾丸和垂体中表达双亲来源的等位基因，即逃脱印记。3。

The probe revealed only weak signals in genomic DNA from other cereal species, wheat,barley, maize, rice and pearl millet, indicating that xPSF31 is a Setaria genus specific sequence.

该探针虽在谷子品种中显示很高的多态性，但与禾谷类的其他物种的DNA杂交信号微弱，表现出较强的谷子基因组特异性。

With genomic DNA of Clostridium stercorarium as a template, a structural gene pel9A encoding pectate lyase was obtained by PCR amplification. The pel9A was cloned into pET28a to recombine an express plasmid pPel9A, and then the plasmid pPel9A was transformed into E. coli BL-21, expressed under the control of T7lac promoter at 37℃.

利用PCR技术从耐热梭状芽孢杆菌中扩增得到产耐热果胶裂解酶的结构基因pel9A，并将其克隆于表达载体pET28a中，最后将此重组质粒转化到受体菌E.coli BL-21中进行表达。

One of the first tools necessary to develop Phalaenopsis orchid genomic research is a orchid BAC library.

因此，本研究的目的为构筑蝴蝶兰 BAC 基因库，进一步分析其特性及建立蝴蝶兰基因实质物理图谱。

Here we report a total of 39.9 Mb of expressed sequence tags from 29 animals belonging to 21 phyla, including 11 phyla previously lacking genomic or expressed-sequence-tag data.

在这里，我们报告从29属21门动物，其中包括11门动物以前所缺乏的基因或表达序列标记的数据动物的39.9表达序列标签MB的总数。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。