查询词典 genome

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

Transposable elements are important components of plant genome and play critical roles in genome evolution.

转座子是植物基因组的重要组成部分，对于研究植物基因组进化等具有重要意义。

Fifty-nine insect species have been included in genome sequencing projects, whole-genome sequences of six insect species, i.e., Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Apis mellifera, Aedes aegypti and Tribolium castaneum, have been reported.

目前有59种昆虫已经列入基因组测序计划，其中6种昆虫（黑腹果蝇Drosophila melanogaster、冈比亚按蚊Anopheles gambiae、家蚕Bombyx mori、意大利蜜蜂Apis mellifera、埃及伊蚊Aedes aegypti和赤拟谷盗Tribolium castaneum）的全基因组序列已经报道。

It is an important enzyme in tricarboxylic acid cycle, consisting of at least 43 proteins,of which, seven proteins are encoded by the mitochondrial genome, while the remainder are encoded by the nuclear genome.

现在普遍接受的观点认为，滋养细胞对子宫螺旋动脉的浸润发生障碍是妊高征病理生理改变的初始环节。

About 477 cM of redtangerine genome, 202 cM of trifoliate orange genome were covered.

母本、父本及双亲共有连锁图分别覆盖基因组总长的477 cM、202 cM和546cM。

Thus it is estimated to represent a nearly 6 - fold coverage of whole Fugu haploid genome and to provide 99. 7% probability to find any unique DNA fragment in the pufferfish genome.

根据这些数据推算，本文库相当于5.8个河豚鱼单倍体基因组(即5.8个库容量)，从本文库中筛选到河豚鱼基因组任一单拷贝DNA序列的几率为99.7％。

However, the use of Aegliops tauschii in improvement of wheat yield trait has not been reported. In this study, T.durum-Ae.Squarrosa amphidiploids was crossed with common wheat PH85-16, then backcrossed with PH85-16. The variation characteristic of the main yield traits of BC1F1 and BC1F2 were analyzed. Then, QTLs affecting yield traits were detected by 130 SSR primer pairs of D genome in BC_1F_1 and BC_1F_2.The result showed chromosomes in D genome from Aegliops tauschii affected yield trait of wheat significantly.

本研究以二倍体小麦粗山羊草与四倍体硬粒小麦合成的双二倍体Am6为亲本，与普通小麦PH85-16 进行杂交，再用PH85-16 进行回交，对获得的回交后代进行了主要产量性状调查，利用位于小麦D 基组的SSR 分子标记进行了分子标记分析，对来自粗山羊草与产量性状相关的数量性状位点进行定位，探讨粗山羊草对小麦产量性状的影响。

Complete genome sequences of 20 viruses in Bunyaviridae loaded from NCBI were analyzed by two biological softwares of DNAMAN and DNASTAR. The results indicated great variability in both nucleic acid sequence and protein structure between plant viruses and animal viruses:(1) only plant viruses could encored NSm in M genome;(2) the length of nucleic acid sequence and protein sequence was different;(3) GC content of nucleic acid of animal viruses was higher than plant viruses of that;(4) protein topology analysis by online software SMART discovered that there was significant difference for the structure of glycoproteins G(subscript nG between animal-infecting and plant-infecting viruses;(5) usually, protein of plant-infecting virus had more complicated structure, more low compositional complexity and had N-Signal peptides except INSV.

本文从NCBI数据库下载具有完整基因组序列的布尼亚科病毒的5个属20种病毒的序列，用生物学软件DNAman，DNAstar进行比对分析，发现布尼亚科病毒属中的植物病毒在核酸序列及蛋白结构上与动物病毒有很大差异：(1)只有植物病毒的M基因组能编码NSm运动蛋白；(2)植物病毒和动物病毒在核酸序列和蛋白质序列长度均有差异，表明该科病毒是进化速度较快的病毒；(3)植物病毒核酸序列的GC含量低于动物病毒；(4)通过SMART网络软件进行蛋白质拓扑结构分析发现植物病毒和动物病毒在糖蛋白GG的结构上存在显著差异；(5)植物病毒糖蛋白结构较为复杂，有较多的紊乱区域，除INSV外，其他病毒都具有N端信号肽。

RESULTS: Useful features of Genomorama include genome search by DNA hybridization (probe binding and PCR amplification), efficient multi-scale display and manipulation of multiple genomes, support for many genome file types and the ability to search for and retrieve data from the National Center for Biotechnology Information Entrez server.

结果：Genomorama的有用的特征包括通过DNA杂交进行基因组搜索，多重基因组的有效的多尺度展示与操控，支持多种基因组文件类型，及从国家生物技术信息中心的Entrez服务器搜索和检索数据的能力。

The SINEs RNA were transcripted by polymerase III; the transcripts can be reversely transcribed and the DNA products inserted into the host genome. The insertion of a SINE can be asseumed to be unidirectional since there is no known mechanism to precisely remove SINEs after their fixation of the host genome.

SINEs插入基因组后，很难在其相同位点精确的去除，能相对稳定地遗传；因此为系统进化分析提供了良好的分子标记，并被认为在物种50Mys的进化事件上能提供比较可信的进化信息，尤其是在科属以下的物种中。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。