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Transposition of transposable element increases its copy number and thus restructures the genome.

由于转座子的跳跃而使其拷贝数增加,从而使植物基因组的可塑性增大。

Transposable elements are regarded as a kind of movable DNA sequence with multiple copy number in genome that can be transmitted by the help of specific transcriptase.

转座子是一类散布在基因组中序列重复的 DNA片段,它们可以通过特定转座酶在基因组中移动。

One role of DNA methylation is to turn off repetitive genes, such as transposable elements that can move or spread throughout a genome and disrupt other gene functions if left unchecked.

DNA甲基化的一个作用就是关闭一些重复的基因,例如一些在不加抑制的情况下能转移或者延伸到基因组的其他地方去阻断基因的转座子。

To better understand and utilize this kind of transposable element, both bioinformatics and molecular biology methods are employed to identify and characterize MITEs in wheat genome.

为了更好地理解和利用这类转座元件,本文综合利用生物信息学与分子生物学方法,对小麦基因组中MITE元件的分子特征进行了研究,并利用它开发出了一种新型的分子标记。

Lastly, specimens contain HPV16 DNA were digested with restricted enyzme BamHI, and done with southern transprint to hybridize with digoxigenin-labeled probe of HPV16 genome through nuclear acid hybridization. Results (1)Among 60 cervial carcinoma specimens,the HPV16 E5 positive rate is 33%(20/60) by PCR, 70%(42/60)、58%(35/60)by nuclear acid dot hybridization with HPV16 E5 probe and HPV16 probe .

结果(1)PCR法 60例宫颈癌组织中HPV16 E5 片段的阳性率为33%(20/60);(2)斑点杂交法60例宫颈癌组织中HPV16 E5阳性率为70%(42/60),HPV16阳性率为58%(35/60);(3)宫颈癌组织中HPV16 E5基因的突变率为40%(8/20),迁移位置不甚一致,随机抽取一例E5变异的PCR阳性产物进行测序分析,结果发现在E5片段中有2处变异, nt3979A→C颠换,nt3988T→C转换,导致E5编码区出现1个氨基酸的有义变异。

In addition, Germany from 14 countries to participate in the 64 research teams of international scientific research team released the first complete genome sequence beetle, the beetle called Tribolium castaneum is a major agricultural pest.

此外,有德国参与的由14国64个科研小组组成的国际科研团队发布了首个甲虫全基因组序列,该甲虫名为赤拟谷盗,是农业上的一大害虫。

Researches in genodermatoses has rapidly advanced in China in recent ten years. The causative genes involved in multiple familial trichoepithelioma and primary erythermalgia have been found. Two indepen- dent genome-wide scans with DNA markers have been performed to detect genetic linkage related to psoriasis and vitilig...

近几年来我国皮肤遗传病研究也取得了飞速发展,如发现家族性多发性毛发上皮瘤和原发性红斑肢痛症的致病基因,成功进行了汉族人群银屑病和白癜风易感基因的定位,为搜寻其易感基因奠定了坚实基础。

Then we compared cleavage rate and blastocyst rate among different groups. After that we evaluated the quality of blastocysts by using ICM/TE (Inner cell mass/Trophectoderm cells) ratio via staining with propidium iodide and Hoechest333258, and analyzed the level of genome methylation and histone acetylation by immunofluorescence.

比较各组受精卵的卵裂率和囊胚发育率;并应用碘化丙啶和Hoechest333258对囊胚进行染色,利用ICM/TE值评价各组胚胎体外发育的质量;同时将囊胚进行免疫荧光染色,观察其基因组甲基化和组蛋白乙酰化的水平。

Extraction of genome DNA ligneous plant ; Ulmus Lamellosa ; Elaeagnus mollis ; Ziziphus Jujuba

基因组DNA提取;木本植物;翅果油树;脱皮榆;酸枣

With the quick progress of the Human Genome Project , a great amount of unannotated human and other organism DNA data has be...

自从人类基因组计划实施以来,大量人类及其它模式生物的DNA序列已被测定,这些序列急需被准确地标定。

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