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In silico cloning was a new technique of gene-cloning which developed with genome project and EST project via bioinfomatics method.

电子克隆是随着基因组计划和EST计划的实施而发展起来的,是利用生物信息学手段进行基因克隆的新方法。

In silico cloning was a new strategy of gene cloning developed with the development of genome, EST projects and bio-informatics.

电子克隆是基因克隆的新策略。

The results show that NPT II had already integrated into the genome of Sinningia speciosa, and the transformation efficiencies of tMEK2 and tMEK2MUT were 1.7% and 0.7%, respectively.

野生型、突变型MAPKK基因的转化效率分别为1.7%和0.7%。

It has also become a good model system to study cross-talk of different plant hormones with the development of genetics and whole tomato genome sequencing from International Solanaceae Genomics Project.Jasmonic acidis a fatty acid-derived signal and exists in the plant kingdom extensively.

番茄是最重要的蔬菜作物之一,随着番茄遗传学研究和国际茄科基因组计划(International Solanaceae Genomics Project,SOL)的发展,番茄已成为研究植物激素相互作用的理想模式植物。

Sequence analysis and homology alignment showed that MD1 had 93% similarity with matK in maize chloroplast genome, a gene encoding maturase included in type Ⅱ intron splicing of RNA transcript; MD2 had 99% similarity with gene PP2C, encoding serine / threonine protein phosphatase type 2C in extremely drought tolerant Sporobolus stapfianus; and MD3 had 99% similarity with rice gene encoding metacaspase, belonging to aspartic acid specific cysteine caspases.

序列分析和同源性比对表明,MD1与玉米叶绿体基因组中编码参与RNA转录本Ⅱ型内含子剪切的成熟酶的matK基因有93%的相似性,MD2与极端耐旱的Sporobolus stapfianus的丝氨酸/苏氨酸2C型蛋白磷酸酶基因PP2C的相似性达99%,MD3与属天冬氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase基因有99%的相似性。

Results of sequence and homology analysis show that MD 1 has 97% similarity with mark in maize chloroplast genome,a gene encoding RNA maturase involved in group Ⅱ intron splicing of RNA transcript;MD2 has 99% similarity with the gene serine/threonine phosphorylase 2C in Sporobolus stapfianus;and MD3 has 99% similarity with rice the gene encoding metacaspase,an arginine/lysine-specific cysteine protease.

序列分析和同源性比对表明,MD1与编码成熟酶的玉米叶绿体基因matK有97%的相似性,MD2与极端耐旱植物Sporobolus stapfianus编码丝氨酸/苏氨酸蛋白磷酸酶的PP2C基因有99%的相似性,MD3与属精氨酸/赖氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase酶基因有99%的相似性。根据MD2片段序列,结合电子克隆和RT-PCR方法,克隆出一条1731 bp的全长cDNA序列,它编码388个氨基酸。

Gekko gecko ; Squamate ; Complete sequence of mitochondrial genome ; Gene organization

大壁虎;有鳞类;线粒体基因组全序列;基因结构

We had preliminary researched on the mechanism of Stylosanthes guianensis cv. Mineirao resistant to anthracnose, a specific fragment of SG2950-1 was acquired from genome DNA of Stylosanthes guianensis cv. Mineirao by the technique of AFLP in our laboratory, we acquired both ends of the fragment of SG2950-1 by RACE from Stylosanthes guianensis cv.

我们已经对Mineirao柱花草的抗病机理进行了初步研究,并用AFLP分子标记技术从其基因组DNA中获得了一特异片断SG2950-1,利用快速扩增cDNA末端技术将基因组DNA特异片段的两端从Mineirao柱花草扩增出来,经测序后拼接得到MSRA基因的全长序列。

By PCR amplification and southern blot analysis, the extrogenous gene with specific promoter was confirmed to integrate into the supersweet maize genome DNA with single-copy, and transformation frequency could reach as high as 9.09%.

经PCR和Southernblot分析,证明抗虫基因已经整合在超甜玉米基因组中,并且为单拷贝。T1代植株PCR检测结果表明,Bt基因能够在转基因后代中稳定遗传,基因分离符合1∶1的孟德尔遗传分离规律。

In order to identify precisely the species of parasites eggs in kimchi and other related products and distinguish from the plant-parasitizing nemotode eggs, we researched the detection technique of Ascaris egg looked as the highest polluting risk of kimchi. The specific fluorescence quantity PCR method was established by using the Ascaris suum genome DNA as positive control,and the optimum way of breaking the eggshells in liquid nitrogen and thawing at 37℃, and the detection method of single egg.

为了精确地对从泡菜及相关产品中发现的寄生虫卵进行种类鉴定,同时与植物本身的线虫卵进行区别,我们在上述研究的基础上,以污染泡菜风险最高的蛔虫卵作为检测目标,进行了模拟阳性泡菜样品中携带蛔虫卵的荧光PCR检测技术研究,即以猪蛔虫基因组DNA为模板,建立了蛔虫特异性荧光PCR检测方法。

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