查询词典 genome

Personality traits were evaluated by Eysency Personality Questionnaire that was compiled by Eysency and revised by Gong. Standard scores of Psychoticism, Extraversion and Neuroticism were calculated, and taking 50 points as a boundary. Each dimension was assigned into 2 grades non-psychotic type (P0) psychotic type (P≥50), introversion type (E0), extraversion type (E0), non-nervous type (N0) and nervous type (N≥50). 5 mL ulnar vein blood was obtained without adding decoagulant, and then serum was removed. Genome DNA was extracted from blood clot by conventional chloroform for PCR analysis. PCR product was sequenced by pyrophosphoric acid. SNP was determined by PSQ96 MA sequenator. Allele frequency was analyzed by mass spectrometry.

人格评定采用Eysency编制，龚耀先修订的艾森克人格问卷、计算精神质，内外问，神经质3个个性维度的标准分，并以50分为界，将各维度分为两个等级：非精神病倾向型（精神质0）、精神病倾向型（精神质≥50）；内倾型（内外向0），外倾型（内外向≥多50）；非常经质倾向型（神经质0），神经质倾向型（神经质≥50）取肘静脉血5 mL，不加抗凝剂，弃血清，血凝块常规酚氯仿提取基因组DNA，进行PCR分析PCR产物应用焦磷酸测序PSQ96 MA测序仪进行SNP检测，用质谱法进行及等位方基因频率分析。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

Copies of the T-DNA, suggesting that a total 65505 sites in rice genome were targeted. 3. Histochemical assay of different organs (leaf, leaf sheath, peduncle, stamens, rachilla, ovules, palea/lemma, root, etc.) revealed various patterns of the reporter gene expression, ranging from expression in only one tissue, in multiple tissues, and simultaneously in majority of the tissues.

组织化学检测不同组织/器官（叶、叶鞘、穗轴、雄蕊、小穗轴、子房、稃、根等）表明，不同Enhancer trap系中报告基因GUS呈不同的表达模式，有的只在一个组织中表达，有的在多个组织中表达，有的在所有组织中均表达。

The 9.7 MB linear genome of Rhodococcus sp.

RHA1红球菌基因组编码24个潜在的非核糖肽和7 polyketides ，所有的未知函数[ 20 ] 。

The genome sequence as a common backbone seres as a scaffold for the integration of data, while, in a complementary effort, these data are enhanced through the application of state-of-the-art bioinformatics tools.

基因组序列作为一个基本的骨架可以成为整合其他数据的平台，然而作为补充，这些数据作用还应该通过生物信息学工具的利用得到加强。

Through seriate genome-wide mRNA expression data, similarity between two genes could be measured.

通过分析酵母细胞的大规模基因表达谱数据，获得不同基因间表达的相关性。

The results obtained can be used for setaria genetics and genome research, and are important for related sepecies in connected fields.

本研究筛选的ISSR引物及建立的优化反应体系可用于谷子等狗尾草属植物遗传多样性和基因组研究，并对其他近缘作物有指导意义。

Mr Shreeve does the same for the publicly funded Human Genome Project, probing its organisational flaws and petty rivalries.

Shreeve先生也是同样对待人类基因组项目的，查看他的组织缺陷和小错误。

It would take another three years before the publicly funded project produced a complete sequence of the human genome, and many more again before what Mr Shreeve calls the "muck of ego, political posturing and accusations of fraud" began to fade away.

也许还要花三年的时间公众基金才能完成人类基因完整顺序，或者更多。Shreeve说了解自我的热潮开始退去。

In silico cloning was developed with the genome and EST project. It is a new method of gene cloning by bioinformatics.

电子克隆是随着基因组计划和EST计划实施而发展起来的利用生物信息学手段进行基因克隆的新方法。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新