查询词典 genome

In our study,two important lepidopteron pests,beet armyworm and cotton bollworm were surveyed for their endogenous piggyBac.As a result,endogenous piggyBac elements were found in these two insects,and one of the piggyBac elements isolated from the genome of cotton bollworm was intact and thought to be potentially active.1 Clonging and sequence analysis of piggyBac from beet armywormUsing PCR technique,with degenerate primers,a DNA fragment of piggyBac-like element was cloned from the genome of beet armyworm,spodoptera exigua hubner.The DNA fragment was 456bp in length,and the deduced amino acid sequence shares 50%- 78% similarity with other piggyBac elements from insects.But one stop code was found in this DNA fragment.

由于转座子很难用常规的RT-PCR和RACE技术进行克隆鉴定，故本研究在兼并引物PCR的基础上，利用inverse PCR和vectorret PCR技术，不仅调查了鳞翅目两种重要的农业害虫甜菜夜蛾和棉铃虫的內源piggyBac存在情况，同时克隆到了內源性的piggyBac转座子，并且从棉铃虫中获得了一个结构完整、具有潜在活性的piggyBac转座子HaPLE1.1甜菜夜蛾piggyBac转座子基因的克隆与序列分析采用PCR技术，利用兼并引物，从甜菜夜蛾基因组中克隆出一个內源性piggyBac类似因子的DNA片段，并命名为SePLE。

Analysis of the obtained genome amplification showed that DNA after PCR presented obvious polymorphism, and the results were the same using the same primer to amplify the larva and imago of the same species. It indicates that RAPD technique can be applied to the identification of dry samples and dipped larva sample of longicorn beetles as well as the research of phylogeny. These three methods for extracting genome DNA from different species of longicorn beetles at different preserved time are feasible.

对所获得的基因组扩增分析表明，经过PCR之后的DNA能呈现明显的多态性，同种天牛用同一引物扩增幼虫和成虫所获得的结果相同，可以认为，RAPD技术可以应用于天牛成虫干标本及幼虫浸渍标本的鉴定及系统发育研究中，也证明了这3种方法用于提取不同保存时间、不同种天牛基因组的DNA是切实可行的。

MGD data content includes comprehensive characterization of genes and their functions, standardized descriptions of mouse phenotypes, extensive integration of DNA and protein sequence data, normalized representation of genome and genome variant information including comparative data on mammalian genes.

MGD数据内容包括了基因和它们功能的综合特征，小鼠表型的标准化的描述，DNA和蛋白质序列数据的广泛整合，包括与哺乳动物基因的比较数据的基因组和基因组变异信息的归一化的表征。

Since the human genome was sequenced six years ago, the cost of producing a high-quality genome sequence has dropped precipitously.

从六年前人类基因组测序的完成，高品质的基因组测序结果的花费已经明显降低。

The genome of schistosome had been become the subject of a genome characterization initiative.

目前，血吸虫病的防治策略主要有灭螺以预防传播和吡喹酮化疗控制发病。

Chromosome pairing behavior in meiosis of the hybrid F1 was carried out. Results showed that in an average , 1.25 rod bivalents were observed in one PMC, meiotic configuration was 2n=14=11.49Ⅰ+1.25Ⅱ(Xta=1.25) and most of PMCs possessed 1～5 bivalens, indicating that the relatively high homeology was detected between the D genome of Ae.tauschii and the V genome of D.villosum. The morphological differences between F1 hybrids and their parents were significant. F1 plants were highly self-sterile, but partially self-fertile after treated by chromosome doubling technique.

对杂种F1花粉母细胞减数分裂中期Ⅰ染色体配对行为进行观察发现，"节节麦×簇毛麦"杂种F1平均每PMC有1.25个棒状二价体，染色体的平均构型为2n=14=11.49Ⅰ+1.25Ⅱ(Xta=1.25)，大部分被观察的细胞出现1～5个二价体，表明节节麦D染色体与簇毛麦V染色体间具有相对较高的部分同源配对， D和V染色体之间存在一定的部分同源性。F1植株高度自交不育，经染色体加倍处理后能够自交结实。

The methods for determination of the complete genome sequences of potyviruses, carlaviruses and potexviruses were also established. The complete genome sequences of 13 viruses(20 isolates) were determined while 10 of them were the first report in the world.Five of them including Lily symptomless virus (NC_005138), Lily mottle virus(NC_005288),Onion yellow dwarf virus (NC_005029), Lily virus X (NC_ 007192) and Shallot yellow stripe virus(NC_007433) were selected as the standard sequences of those viruses by National centery of Biotechnology Information, USA.

建立了马铃薯Y病毒属、麝香石竹潜隐病毒属和马铃薯X病毒属成员的基因组全序列扩增技术，完成了13种不同植物病毒近20个分离物的基因组全序列测定，其中10个病毒的基因组全序列为国际首次报道，百合无症病毒(NC_005138)、百合斑驳病毒(NC_005288)、洋葱黄矮病毒(NC_005029)、百合X病毒(NC_ 007192)和葱黄条病毒(NC_007433)等5条序列被美国国家生物技术信息中心列为相关病毒标准序列的有。

To establish a new method to isolate genomic regulatory sequence using unpurified nuclear extract, here a new strategy was developed based on modified whole genome PCR and electrophoresis mobility shift assay. The principle is as follows. After sonication and ligation with DNA linker, human genomic DNA is amplified and labeled with isotope by whole genome PCR.

为了建立一种利用未经纯化的细胞核粗提物就能从人基因组中快速、大量筛选DNA调节片段的新方法，本研究对原有的全基因组PCR技术进行改进，结合凝胶阻滞实验，建立了筛选DNA调节片段的新方法。

Medicago truncatula has become a useful model plant for biology and genome of Legumes,since it has a small diploid genome(2×8),rapid reproductive cycle,autogamous fertilization,nitrogen-fixing,and ease of transformation.

由于蒺藜苜蓿具有高的遗传转化效率、生长期短、染色体组为2×8、基因组小、自花授粉、固氮等特点，使它成为豆科生物学和基因组学研究的模式植物。

In this work, we used the sequences of nuclear genes (ITS, Adh2, Adh3 and Waxy) and cpDNA (trnL-F and matK) to explore the origin of Elymus species and the Y genome, and to reveal the mechanism of the geographical differentiation of the StY-genome Elymus species.

本文通过核基因ITS、Adh和Waxy基因以及叶绿体基因trnL-F和marK的序列分析了披碱草属相关二倍体种的系统发育关系和披碱草属植物的起源。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。