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Five methods namely improved CTAB method, CTAB-silica extraction method, SDS method, low pH extraction method with high salt and Zhou shiliang method were adopted to extract genome DNA from the leaves of Citrus aurantium. And three methods including ultraviolet spectrophotometer, agarose gel electrophoresis and SSR-PCR amplification were used to detect extracted sample DNA.

以枳壳叶片为试验材料,分别采用改良CTAB法、CTAB-硅珠法、SDS法、高盐低pH值法和周世良法5种方法提取枳壳基因组DNA,并通过紫外分光光度计、琼脂糖凝胶电泳及SSR-PCR扩增3种方法对所提取的DNA样品进行检测。

Amaximum of two bivalents formed by autosyndesis within B genome at diakinesisappeared in all combinations.

六倍体Ⅰ、Ⅱ、Ⅲ与诸葛菜杂交分别获得30,4及3株杂种单株,每个单株均来自不同的胚。

These two avirulence genes were linked in the segregation population. 2. The genome DNA of wheat powdery mildew was extracted from isolates derived from single colonies.

对小麦白粉菌 DNA 的 RAPD 分析的结果未表明 RAPD 标记的 DNA 多态性和无毒性多态性之间直接的相关关系,这一结果与前人对其它真菌病原菌的研究结果相同。

Thinopyrum intermedium,one of the relative species of common wheat,is a kind of dominant wheat germplasm resource for the resistance to stripe rust in wheat genetic improvement. With using Chinese spring phlb as recurrent parents and awnless Zhong 4(a partial chromosome of Thinopyrum intermedium)as donor parents,that is backcross selection,the offspring BC_2F_(7~9)were acquired.Cytology check and genome in situ hybridization, were used for the translocation lines and substitution lines detection.

本研究采用常规杂交并辅抗性鉴定等方法,以中国春phlb突变体为轮回亲本,利用无芒中4,转育中间偃麦草的抗条锈性,并通过细胞学和DNA原位杂交检测,鉴定和筛选普通小麦—中间偃麦草的抗条锈病异代换系或易位系。

In order to investigate whether fragments of Sugarcane bacilliform virus were integrated into Saccharum inter-specific hybrids genome, a DNA fragment homologous to SCBV was amplified from a hybrid sugarcane plant Saccharum inter-specific hybrids var.

为研究甘蔗杆状病毒Sugarcane bacilliform virus的基因组片段是否整合进杂种甘蔗Saccharum inter-specific hybrids基因组,以采自广西甘蔗研究所的1株杂种甘蔗植株叶片总DNA为模板,根据已报道的甘蔗杆状病毒基因组序列设计多条特异引物。

Particular attention will bepaid in the field of gene expression regulation to high-throughput screening andidentification of cis-acting elements within the genome and to investigation ofinteraction manners of these regulatory sequences with regulatory proteins in theconditions of in vivo chromatin contexts.

如何在体内的染色质环境下,高通量、大规模地筛选与检测基因组内的顺式作用元件并研究其与反式作用因子间的相互作用,是当前和今后一个时期内基因表达调控领域内的研究重点之一。

Bicolor genome showed higher homologous in repeat sequence than S. propinquum with S. dochna.

栽培高粱与拟高粱基因组间重复序列的同源性要比甜高粱与拟高粱间重复序列的同源性高。

The research of the correlation between TCM Syndrome and genome is just exploring the bimolecular background of TCM syndrome using the method of bioinformatics.

中医证候-基因组研究即是借用了生物信息学的方法来探索中医证候的生物学本质。

The biosystematic relationship between two species of Nelumbo Adans.was studied by the method of karyotype analysis,genome analysis and silver-staining of interphase neclei in Nelumbo lutea Pers.

对莲属美洲黄莲和莲的3个品种的核型分析、染色体组分析和间期核银染,研究了莲属两个种的系统关系。莲属两个种的核型大致近似,均有2对具随体染色体。

The 12940 bp nucleotide sequence of the mitochondrial genome of a brachyura dacapod, Eriocheir japonica sinesis, was determined.

测定了短尾类十足目动物中华绒螯蟹的线粒体基因组12940 bp的核苷酸序列。

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