查询词典 genome

Full genome of HCV ; Clone ; Transfected cell culture system ; Recombinant vaccinia virus

HCV全基因组；克隆；转染细胞体系；重组痘病毒

Coli genome was amplified by using PCR method. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the Mn-SOD protein expressed by pET-28a vector and purified was injected into rabbits to prepare polyclonal antibody. Western-blot was used to detect the effect of polyclonal antibody.

coli基因组中扩增Mn-SOD基因编码区，将它克隆到原核表达载体上进行大量表达和纯化，再用纯化的蛋白对新西兰大白兔进行背部多点注射，40d后取其血清，用Western-blot印迹实验测定抗体效果。

Methods: The coding region of superoxide dismutase was amplified using PCR method from the E.co1i genome. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the cloned coding region of Mn-SOD was inserted into the expression vector PET-28a to form the recombinant plasmid PET-28a-Mn-SOD and was then transformed into E.coli BL21 for expression.

用PCR方法从大肠杆菌2号基因组中扩增Mn-SOD基因编码区，克隆到pUCm-T Vector，测定核苷酸序列；再将基因编码区克隆到原核表达载体PET-28a，构建含Mn-SOD基因的重组表达质粒，转化到大肠杆菌BL21中进行IPTG诱导表达。

In order to prevent linear PCV2 cyclization, PCR mutagenesis was used to construct the first molecular clone (pSK-2PCV2) by ligating two copies of the complete PCV2 genome with the pBluescript SK vector. In addition, pSK-PCV2 and ds-PCV2 were constructed. PK-15 cells were transfected with above three infectious clones.

本研究应用PCR诱变技术解决了外源片段易于自连的难题，成功将2个PCV2 SD1株全基因组(DQ346683)头尾相接插入到真核生物表达载体pSK的多克隆位点中，构建重组质粒pSK-2PCV2；另外课题组成功构建含单个PCV2全基因组的pSK-PCV2和自身环化质粒ds-PCV2。

objective: to achieve the soluble expression of mn-sod gene in e.coli and assay the enzyme activity of the expressed product. methods: the coding region of superoxide dismutase was amplified using pcr method from the e.coli genome. the pcr product was cloned into puc19-t vector and sequenced.in addition,the cloned coding region of mn-sod was inserted into the expression vector pet-28a to form the recombinant plasmid pet-28a-mn-sod and was then transformed into e.coli bl21 for expression.

超氧化物歧化酶(superoxide dismutase，sod)是细胞体内歧化超氧阴离子自由基(o-2)的一个抗氧化酶，按其结合的金属性离子根据其中金属辅基的不同可分为4类：cu/zn-sod、mn-sod、fe-sod和ni-sod，它们通过催化超氧阴离子自由基0-2发生歧化反应，达到清除o-2的效果，具有防御氧毒性、增强机体抗辐射损伤能力、防衰老以及治疗某些肿瘤、炎症、自身免疫疾病等功效，广受国内外科研工作者的关注和重视[1]。

What, Venter wondered back then, was the minimum genome an organism needed to survive and reproduce?

那是Venter考虑的是一个有机体所要生存和繁衍，最少的基因组是多少？

Dentium so different from its relatives, Ventura and colleagues sequenced the bug's genome.

dentium与它的同类与此不同，温特勒和他的同僚们排出了这种细菌的基因图谱。

Xeroderma pigmentosum C is a DNA repair factor essential for global genome repair in nucleotide excision repair.

XPC为DNA修复因子，其为核酸切除修复途径中整体基因修复的必要因子。

Objective To analyze the genetic characteristics of structural genome of yellow fever vaccine virus strain in China.

目的 分析中国使用的黄热疫苗株病毒结构基因片段的遗传特征。

It can grow all right in high salt Since twenty century , molecular biology and bio-technology have a large development , agriculture and physic have been speedily development too ,especially after the plan of human genome was successfully implemented ,more people engaged in bio-technology industry .but the area of the bio-technology study was very extensive , Ocean Rhodotorulas have been studied by people at beginning, on the end of 1980s , Ocean Rhodotorulas separation from sea and Japan was the first succeed in cultivation by zymolysis , the Ocean Rhodotorula of cultivation was used for a good nutrimental to feed for fish and shrimp.

二十世纪以来，分子生物学与生物技术得到了迅猛发展，在农业和医药业都得到了飞速发展，尤其是人类基因组计划的成功实施，使人们更多的从事着生物技术行业，但是生物技术研究的范围很广，所以对于海洋红酵母的研究还处于刚刚起步阶段，上世纪80年代日本最早分离到海洋红酵母，然后发酵培养，用做鱼虾的营养饲料。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新