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Random amplified polymorphic DNA markers were used to measure genetic diversity and genetic structure of 3 natural populations of Carya dabieshanensis .238 loci were identified by 20 RAPD primers,out of which 162 loci were polymorphic and accounted for 68.1% of the total genetic diversity at species level.

摘 要:利用RAPD分子标记技术检测了大别山山核桃3个天然居群的遗传多样性和遗传结构。20条10bp随机引物共检测到238个扩增位点,其中多态性位点162个,多态位点百分率为68.1 %。

The results showed that the clonal reproduction was preponderant, but it has still a high genetic diversity (Ho=0.7148). At the same time, Corylus heterophylla Fisch genetic individual was found. A single cluster was basically a genetic individual; adjacent clusters were basically the same genotype and clonal reproduction has larger percentage.

结果表明:平榛虽然克隆繁殖占有优势,但仍具有较高的遗传多样性(Ho=0.7148);同时确定了平榛的遗传个体,同1株丛内个体基本上为1个独立的遗传个体,相邻的株丛个体基因型基本一致,克隆繁殖比率较大。

Nei'genetic distance showed Schima superba and Cryptocarya chinensis hadsmall genetic distance between every two subpopulations of each own,but Castanopsischinensis had high genetic distance between every two subpopulations of own.

Nei遗传距离结果表明,荷木和厚壳桂两两亚种群间都有非常小的遗传距离,而锥栗却有大的两两亚种群间遗传距离,表明荷木和厚壳桂亚种群间个体的差异小,有较少的位点频率发生变化;而锥栗亚种群间个体的差异大,有较多的位点频率发生变化。

Asymmetric over-introgression of the indica alleles on chromosome 7 and many specific regions on other chromosomes in the japonica genetic background is consistent with the general trend observed in the RIL and F_2 populations of the same cross, suggesting a high genetic load in the japonica gene pool, and japonica genome experienced a very severe bottleneck by artificial domestication.2 Genetic background effect on QTL expression of grain weight and its related traitsGenetic background effect on main-effect QTL and digenic epistatic QTL mapping for GWT and its related traits including GL, GW and GT was dissected by a large set of reciprocal introgression lines and recombinant inbred lines derived from Lemont and Teqing in Beijing environment.

籼粳相互导入具有不对称性,粳稻向籼稻背景的平均导入频率与期望值相仿,相反籼稻向粳稻背景的平均导入频率大大高于理论预期,位于第7染色体和一些其它染色体的特殊区段存在籼稻等位基因的超导入现象,在来自相同亲本的重组自交系和F_2群体中也观察到同样的导入趋势,表明粳稻基因组具有较大的遗传负荷,粳稻品种在人工驯化过程中曾经历过一个非常剧烈的遗传瓶颈。2水稻籽粒相关性状的主效和互作QTL表达的遗传背景效应利用Lemont和特青培育的双向导入系和重组自交系群体,在北京环境下定位影响千粒重及其相关性状如粒长、粒宽和粒厚的主效和互作QTL,分别在特青背景导入系、Lemont背景导入系和重组自交系下检测到影响千粒重及其相关性状的主效QTL分别为33个、27个和13个,互作QTL分别为4个、8个和23个,其中仅有6个主效QTL(QGwt5,QGl1,QGl3b,QGl4,QGw7和QGw8)在籼粳两个不同背景下共同表达,3个主效QTL(QGwt5,QGwt7a和QGt5)在导入系和重组自交系中共同表达,分别占检测到影响这些性状QTL总数的11.1%和4.3%,未检测到在3个背景下相同表达的互作QTL。

These genes are related to many plant functions, indicating that the heterosis was related with many metabolization pathways.1.2 The quantity of discrepant genes showed that the genetic discrepancy was notalways the same with the discrepancy of expressed genes, indicating that small-scale genetic discrepancy maybe result in large-scale genes discrepantly expression; in other way, some genetic discrepancies might not embodied on the discrepant genes in the experiment.1.3 Nine and eight discrepant genes were found respectively in the superior bulkand minor bulk in the dominant level.

1.2 各张芯片差异表达基因数量表明遗传差异与表达谱差异不尽一致,可能较小的遗传差异可以造成表达谱的巨大改变,而有些遗传变异可能没有在表达谱上表现出来。 1.3 从优势集团和负优势集团中分别找到9个和8个具显性表达水平的差异表达基因,研究表明显性表达水平的差异表达基因的聚合与杂种优势的表现没有相关性。

The research areas cover genetic and physiological mechanisms of functioning marine organisms; principles of marine biotechnology; genetic and endocrinological mechanisms in marine fish and invertebrates; genetic polymorphism of some marine and fresh water species; biological role of bacterial bioluminescence in the marine environment etc.

其研究范围广泛,覆盖了多个学科,包括功能性海洋生物的遗传和生理机制、海洋生物技术原理、海洋鱼类和无脊椎动物的遗传和内分泌机制、海水和淡水某些生物的遗传多态性、以及海洋环境中发光细菌的生物学作用等。

The unconditional and conditional analyses of genetic models and the corresponding statistic methods, including endospermic, cytoplasmic, and maternal plant genetic systems, were used to analyze the genetic relationships between protein content and the appearance quality traits of indica rice (Oryza sativa L.).

应用包括胚乳、细胞质和母体植株基因的遗传主效应以及基因型×环境互作效应的数量性状遗传模型及非条件和条件分析方法,研究了籼稻稻米蛋白质含量与外观品质性状间的遗传相关性,进一步揭示了籼稻糙米重、稻米直链淀粉含量对稻米蛋白质含量与外观品质性状间遗传相关性的影响。

The result of genetic analysis conveyed that in partial sequences of mtDNA Cyt b gene there were no variable sites between the eggs and Japanese halfbeak, and the genetic distance between them was 0, however, many variable sites between the eggs and the other fishes of Beloniformes were exised, the variable rate was 18.72-21.3%, and the genetic distance between them were 21.9-26.4%.

遗传学分析结果显示:鱼卵与沙氏下鱵鱼mtDNA Cyt b基因片段序列之间无差异,遗传距离为0,而与其它颌针鱼目鱼类序列间差异达18.72~21.3%,遗传距离在21.9~26.4%之间。

In this study, the genetic structure and genetic diversity of cultured and wild Pacific abalone Haliotis discus hannai were analyzed using AFLP technique on the basis of investigation of the inheritance mode of AFLP markers, and genetic linkage maps of female and male H.

本文通过分析皱纹盘鲍AFLP分子标记的遗传模式,利用AFLP技术对皱纹盘鲍养殖与野生群体进行了遗传多样性及遗传分化研究,并对皱纹盘鲍单对交配的亲本及其F1子代进行AFLP和微卫星作图标记筛选,建立了初步的遗传连锁图谱。

The average percentage of polymorphic bands are 72.06% for ISSR and 66.67% for RAPD respectively. The genetic diversity of ginkgo revealed by ISSR (the average effective number of alleles is 1.8780, the average Nei"s gene diversity is 0.4652, and the average Shannon"s information index is 0.6574) are all higher than those by RAPD (the average effective number of alleles is 1.7842, the average Nei"s gene diversity is 0.4317, and the average Shannon"s information index is 0.6211), while the standard errors of the parameters estimated by ISSR were lower. Therefore, ISSR molecular markers are more suited than RAPD molecular markers when testing the genetic diversity of ginkgo populations and determining the genetic relationship of among populations or among individuals which are much similar hereditarily.

本文应用RAPD和ISSR两种分子标记技术,选取中国5个可能的野生银杏居群共计75个样品,对其遗传多样性进行了研究,得出以下结论:(1)用筛选出的12个RAPD引物和10个ISSR引物进行PCR扩增,分别扩增出65条和68条重复性高、清晰的条带,多态性位点百分率分别66.67%和72.06%,ISSR揭示的银杏遗传多样性(平均有效等位基因数目为1.8780,平均基因多样度为0.4652,平均信息指数为0.6574)高于RAPD所得到的结果(平均有效等位基因数目为1.7842,平均基因多样度为0.4317,平均信息指数为0.6211),其所估算参数的标准差要低于RAPD所估算出的值,因此,在研究亲缘关系非常近的银杏物种的遗传多样性并试图确定居群间或个体间的遗传关系时,ISSR分子标记技术比RAPD分子标记技术更为合适。

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