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①Location of WD gene in Ch inese: Using pairwise linkage analysis and multipoint linkage analysis method, w e constructed a genetic map of DNA markers within D13q14.2-3 which refined the location of WD gene by restriction fragment length polymorphism and microsatellite polymorphism analysis;②Screen for mutations of WD gene in Chinese people: we detected the structure of 21 exons of WD ge ne in 45 patients from 39 pedigrees by PCR-SSCP(Single strand conformation poly morphism) and PCR-DNA sequencing technology, found a new mutation in exon 5 and nuclcotide sequence analysis showed it is a T insertion. We also conformed the Arg778Leu in exon 8, the highest frequence mutation point in Chinese people, wit h mutation rate 22.8%in total;③Carrier detection and presymptomatic diagnosi s of WD: Based on DNA recombination technology, we peformed successfully the gen e diagnosis in all individuals of 79 families with WD and built up a helpful spe cific enzyme cut method (PCR-Msp1) to detect the carrier and presympomatic patients in Chinese pe ople with WD.

①WD的基因定位研究:通过RFLP及微卫星多态性分析,应用两位点及多位点连锁软件,建立了中国人WD基因在D13q14.2-3区域的精细遗传连锁图谱,从而首次对中国人WD基因进行了精确定位;②WD基因突变研究:应用PCR-SSCP及DNA测序技术,对39个家系45名WD患者进行该致病基因的21个外显子突变筛选,发现WD基因5号外显子存在新的T插入突变,并证实中国人WD基因的突变热点为8号外显子,突变形式为Arg778Leu,其频率为22.8%;③WD的症状前诊断和杂合子检出:应用DNA重组技术对79个家系进行基因诊断,成功地进行了WD的症状前诊断和杂合子检出,并建立了WD的基因筛选的PCR-Msp 1酶切方法。

This article thoroughly studied an usable widely VRPTW of logistics distribution, and constructed the mathematical model about this problem, designed a new genetic algorithm to solve VRPTW and with C language programming to test it with an example, finally has obtained satisfaction solution, so had guaranteed enterprise not only satisfy the customer's distribution demands, but also caused the vehicles have the shortest routing, as well the cartage expense was smallest.

本文深入研究了VRP中应用日益广泛的VRPTW问题,构建了该问题的数学模型,设计了求解VRPTW的新型遗传算法,并用C语言编程对该算法进行实例测试,结果得出了满意解,保证了物流企业在满足客户配送需求的同时,又使得运输车辆的配送路径最短、运输费用最小。

Objective To study the genetic expression of insulinlike growth factor Ⅱ in cartilaginous tissue in osteoarthritis.

目的 研究骨性关节炎软骨组织中细胞因子胰岛素样生长因子Ⅱ的基因表达的变化规律。

The study aimed to explore the genetic law of litter size of Inner Mongolia White Cashmere Goat.

[目的]探讨内蒙古白绒山羊产羔数的遗传规律。

Progresses in castor biotechnology were summarized in terms of tissue culture and genetic transformation in this paper.

本文从组织培养和遗传转化两个方面并结合本实验室的工作综述了蓖麻生物工程研究的最新进展。

Study of the relationship between genetic distance of 21 castor inbred lines and hete...

遗传距离与杂种优势指数的关系呈曲线关系。

Consequently, genetic polymorphisms in both nicotine metabolism and dopamine catabolism genes may influence smoking behavior, and interact with each other resulting in risk modulation.

因此,在尼古丁代谢和多巴胺分解中的基因多态性可能会影响吸烟行为,它们的相互作用导致风险的调节。

A hybrid fast genetic algorithm (H2O) is proposed on the basis of various characteristics of nature evolution. This algorithm is combined with niche technician, a variety of crossover schemes, multi-populations and catastrophism phenomenons.

为了有效地克服非线性、多参数、多极值的组合优化问题中遗传算法的早熟收敛现象,引入生物的小生境技术和自然界生物多种杂交方式并存、多个子群体并行以及灾变现象而提出了一种综合的快速基因搜索策略。

The genetic variations and polymorphisms of six microsatellite loci were analyzed to determine the population structure and breeding progress of BMY and Brahman cattle. The range of polymorphic information content of six loci was 0.524~0.752. The unbiased expected and observed heterozygosity were similar and were 0.7376 and 0.7396,0.6412 and 0.6537 for BMY and Brahman cattle, respectively. The expected heterozygosity was relatively high in the second generation of BMY in inter se breeding, which was congruent with the breeding progress.

对6个牛微卫星座位的遗传变异及多态性分析,以期了解BMY牛和婆罗门牛的群体遗传结构与育成情况。6个微卫星座位的多态信息含量为在0.524~0.752间,BMY牛、婆罗门牛两个群体的平均期望杂合度和观察杂合度值接近,分别为0.737 6和0.739 6,0.641 2和0.653 7,进入横交固定第二世代的BMY牛期望杂合度值较高,这与我们的育种进展相符。

The disease is associated with inflammatory bowel disease, and genetic factors may play a causative role.

该病与炎症性肠病、遗传因素可能发挥作用的原因。

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