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The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The blood pressure of.1 of pathology physiology mechanism that SAS causes CVD breathes time-out to be able to bring about airframe in elevatory Morpheus anoxic, sympathetic is excessive and excited, element of serous catechu phenolic amine, kidney and hemal endodermis element are elevatory, bring about hemal easy of hemal convulsion; to shrink the function is disorder, can make blood-vessel flowing flesh happening reframes and fleshy, systemic blood-vessel obstruction adds element of the kidney when; is chronic and anoxic, system of hemal and nervous element is activationed, bring about blood pressure to lift. Obstruction of way of energy of life of patient of OSAS of disease of blood of 1.2 low oxygen increases, cause air current to interrupt, breath pauses, at the same time airframe gets used to low oxygen environment gradually, breathing centre drops to low oxygen and sensitivity of disease of blood of tall carbonic acid, breath suspends a frequency increasing, farther aggravating airframe is anoxic. 1.3 heads are self-adjusting the function drops normal person changes quickly in systematic circulation blood pressure when, the head can be passed adjust independently functional generation protects effect, make change of cerebral blood flow not big.

SAS诱发CVD的病理生理机制。1血压升高睡眠中呼吸暂停可导致机体缺氧，交感神经过度兴奋，血浆儿茶酚胺、肾素以及血管内皮素升高，导致血管痉挛；血管舒缩功能紊乱，可使血管平滑肌发生重构和肥厚，全身血管阻力增加；慢性缺氧时肾素—血管紧张素系统被激活，导致血压升高。1.2低氧血症OSAS患者气道阻力增加，造成气流中断、呼吸暂停，同时机体逐渐适应低氧环境，呼吸中枢对低氧和高碳酸血症敏感性下降，呼吸暂停次数增加，进一步加重机体缺氧。1.3脑自动调节功能下降正常人在体循环血压快速变化时，脑可通过自主调节功能产生保护效应，使脑血流量变化不大。

The application of a cation exchange membrane in hydrogen generation by water electrolysis has been studied in this article.

本文研究了阳离子交换膜在碱性条件下电解制氢中的应用。

Set during the politically turbulent summer of '69, story follows an Everyman working at his parents' motel in the Catskills who inadvertently sets in motion what would become the generation-defining concert.

" "设在政治动荡的夏天，'69 ，故事如下1 everyman工作，在他父母的汽车旅馆，在catskills谁不经意地套在议案，什么会成为一代定义音乐会。

Nowadays, many people have already put their eyes on how to buildup and evolve the third generation of the mobile communication system. LTE systems not only requires enhancing performance, but also have to take other important aspects into consideration, such as coexistence on adjacent channels with existing 3G systems. Now the frame structure parameters to buildup and evolve TD SCDMA system, which is presented by CATT, receive much attention.

第三代移动通信系统性能的增强和长期演进已成为目前的研究执点，这种长期演进系统不仅要求性能上的增强，而且还必须考虑与已有3G系统共存的问题，CATT等公司共同提出的对T-SCDMA系统演进的帧结构参数备受人们关注。

The genetic variations and polymorphisms of six microsatellite loci were analyzed to determine the population structure and breeding progress of BMY and Brahman cattle. The range of polymorphic information content of six loci was 0.524～0.752. The unbiased expected and observed heterozygosity were similar and were 0.7376 and 0.7396,0.6412 and 0.6537 for BMY and Brahman cattle, respectively. The expected heterozygosity was relatively high in the second generation of BMY in inter se breeding, which was congruent with the breeding progress.

对6个牛微卫星座位的遗传变异及多态性分析，以期了解BMY牛和婆罗门牛的群体遗传结构与育成情况。6个微卫星座位的多态信息含量为在0.524～0.752间，BMY牛、婆罗门牛两个群体的平均期望杂合度和观察杂合度值接近，分别为0.737 6和0.739 6，0.641 2和0.653 7，进入横交固定第二世代的BMY牛期望杂合度值较高，这与我们的育种进展相符。

Texture mapping was used to leaf and fruit models, while process texture generation was introduced to cylinder-like organs such as caudex and petiole.

该方法已被用来模拟植物叶片的卷曲和萎蔫过程，获得了较好的真实感效果。5、植物攀援特性建模和攀援行为模拟首次可视化地模拟了卷须类园艺植物藤蔓的形态发展过程。

The 100 generation in company of record of 7 big international and gold exited the cavalcade of accuser early, and those who chose coact is double win mode.

七大国际唱片公司中的百代和金牌早早退出了原告的行列，而选择了共同合作的双赢模式。

In this thesis, we propose a CDR circuit that operates at 6Gbps and conform to specifications of SATA generation three.

在本论文中，我们提出一个操作在6Gbps，符合SATA第三代规格的时脉资料回复电路。

Hanna: That's over now, isn't it?

都结束了，对吗

You must be ill. You look so pale.

你一定是病了，你的脸色苍白。