查询词典 gene

Then plant expression vector pBG128 contains a germin gene ,a Nptll gene as the selectable marker and a B -gluceronidase gene as the reporter gene.

但反向克隆的重组子得到的小片段大小为1，567bp，正向克隆的重组子所得的小片段大小为968bp，并命名正向克隆的重组子为pBG128。

The partial nucleotide sequence of the gene fragment showed high homology with the essential spectrin gene of Caenorhabditis elegans and the erythrocyte surface antigen gene of Plasmodium falciparum , when the gene fragment was homologically analyzed in GenBank.

与GenBank中的基因进行同源性分析，其部分核苷酸序列与秀丽隐杆线虫红细胞膜内蛋白和恶性疟原虫红细胞表面抗原高度同源

Gene-activated matrix technology is a non-viral gene therapy strategy. Though with less impressive gene transfer efficiency than viral gene therapy, it has been used to deliver osteogenic factors locally and promote bone regeneration in long bones. The minimal safety concerns of GAM support its use in non-life-threatening conditions such as craniofacial osseous defects. However, the uses of GAM technology in intramembranous bone are largely unexplored.

活化基因基质技术是一种非病毒性的基因疗法，虽基因转移之效率一般不如病毒性基因疗法，但曾被用来局部产生骨生成因子而促进长骨缺损骨再生，其安全性高，应适於治疗颅颜骨缺损等不具生命危险性的状况，惟至今在膜内骨之相关研究仍甚少。

In this study, insect-resistant cry1Ah gene and glyphosate-toleranct 2mG2-epsps gene was used to construct an insect-resistant/glyphosate-tolerance bivalent plant expression vector, glyphosate isopropylamine salt as a screening agent, 2mG2-epsps gene as a selectable maker gene, the vector was transfer into maize immature embryonic calli by microprojectile bombardment, 80 T0 transformed plants were obtained.

本研究利用抗虫基因cry1Ah和耐草甘膦基因2mG2-epsps构建了双价植物表达载体，通过基因枪轰击转化玉米，以2mG2-epsps为筛选标记基因，以草甘膦异丙胺盐作为筛选剂，获得到了抗性较好的T0代转化植株80株。

To study the transcription expression law of Musclin gene and Lipogenic gene in muscle of the cadmium poisoning mice regulated by Vc. The experiment adopted RT-PCR detection method to study the variety of expression level of mice Musclin gene and Lipogenic gene (FAS, PPARγ, TGH).

为探讨维生素C调控Cd中毒小鼠骨骼肌中Musclin基因及生脂基因的转录表达规律，用RT-PCR方法研究小鼠Musclin基因及生脂基因(FAS、PPARγ、TGH)表达水平的变化。

The results showed that resistant gene Ht in F1 heterozygote was the most effective on controlling spots number and size; all monogene in heterozygote could produce spore by moisture culture and resistant gene Ht1 was the most effective; resistant gene Ht1 in medium level, Ht3 in high level, and Ht in low level of vertical resistance of heterozygote, were the most effective on controlling spots spread; while Ht2 in high level of vertical resistance of heterozygote was the most ineffective on controlling spots spread; and all resistant monogene in heterozygote showed superior to their parents on controlling incubation period and gene Ht was more significant than other genes.

结果表明，抗性单基因Ht处于F1代杂合状态时，控制病斑数和病斑面积的效应最强；单基因处于杂合状态时，湿培48h病斑全部能产生分生孢子，且处于同一水平抗性杂合状态时，Ht1控制产孢量的效应最强；Ht1处于中水平、Ht3处于高水平和Ht、处于低水平垂直抗性杂合状态下，控制病斑扩展效应最强；而Ht2处于高水平垂直抗性杂合状态下，控制病斑扩展效应最弱；抗性单基因处于杂合状态下，控制潜伏期多表现超亲现象，Ht控制病害潜伏期比其它单基因显著。

Majority of acute leukemias in infant, either acute lymphoblastic leukemia or acute myeloblastic leukemia, posses a chromosomal translocation affecting the 11q23 chromosome region which specifically inoles the mixed-lineage leukemia gene.1-3 Most pediatric leukemias with MLL rearrangement clearly hae a remarkably short latency.1,4 MLL gene rearrangement is also associated with secondary leukemias of patients preiously treated with the topoisomerase II inhibitors.4 The latency of these secondary leukemias is similarly ery short.4 Of note, the concordance rate of leukemia with MLL rearrangement in infant monozygotic twins approximates to 100%,1,4 and identical breakpoint in the MLL gene was shared in these pairs of identical twin infants with concordant ALL.1,4 Moreoer, the unique and clonotypic MLL fusion gene was detectable in neonatal blood spots for Guthrie cards from non-twined indiiduals who subsequently deeloped ALL.1,4 These obserations indicate not only that MLL fusion is generated in utero but also that MLL fusion proteins could be capable of inducing leukemic transformation with few, if any, secondary mutations.2,3,4 Greaes et al speculate that an MLL fusion protein somehow promotes rapid transition to full-blown disease in patients ia ery rapid clonal expansion, genetic instability, or inhibition of DNA damage repair.4 In general, for clonal expansion of malignancies, tumor cells often hae acquired strategies that escape immune sureillance of the hosts.5,6 Immune escape mechanisms also contribute to the failure of graft-ersus-leukemia effect after allogeneic hematopoietic stem cell transplantation.7 Therefore, leukemia cells could acquire some immune escape mechanisms during leukemogenesis.

绪论 绝大多数的婴儿白血病，不管是急性淋巴性白血病或是急性骨髓性白血病，在染色体11q23部位有染色体易位的情况；这个部位的染色体易位牵连了混合谱系白血病基因。大多数具有MLL基因重排的儿童白血病潜伏期明显短很多。MLL基因重排也和经拓扑异构酶II抑制剂治疗后的继发性白血病有关。这些继发性白血病的潜伏期类似地都非常的短。很重要的是，单卵双胞胎婴儿同时患有或同时免于MLL基因重排阳性的白血病的一致性接近100%；并且同样患有ALL的同卵双胞胎的MLL基因的断裂点是一致的。而且，这种独特的克隆特异性的MLL融合基因能够从那些得ALL的非双生个体出生时的血斑标本中检测到。这些发现表明MLL融合基因产生在胎儿还在子宫的是后，而且MLL融合蛋白能过和其他的基因突变一起诱导白血病的产生。Greaes 等推测MLL融合蛋白在某种情况下同过快速克隆增殖，遗传的不稳定性或是DNA损伤修复的抑制促使疾病迅速地全面爆发。恶性肿瘤细胞的克隆增殖通常已经获得了逃避机体免疫监视的能力。免疫逃避机制也归因于异体外周血干细胞移植后移植物抗白血病作用的失效。所以，白血病细胞在白血病的产生过程中可能获得了某些免疫逃脱机制。

The cry8C gene from Bt strain HBF-1 was cloned and sequenced. There was one amino acid difference with cry8Cal gene from Bt strain Buibui and it was designed as cry8Ca2 gene (AY518201) by International Bt Insecticidal Protein Gene Nomenclatural Committee.

从菌株HBF-1中克隆的cry8C基因与cry8Cal基因有一个氨基酸的差异，被国际Bt杀虫晶体蛋白基因命名委员会命名为cry8Ca2基因。

In order to understand the function of this gene within short time, we also constructed yeast (Schizosaccharomyces pombe vector to analyze the gene function, but the result investigated that over expression of this gene could not increase the length of yeast cell. It suggested that expression of the gene is not a direct reason in cell elongation.

为了在短时间内初步研究该基因的功能，构建了酵母表达载体，利用裂殖酵母表达系统对该基因的功能进行活体分析，没有发现该基因对单核的酵母细胞的伸长有明显影响。

All the LOH on 〓 and 〓 was observed in invasive ductal carcino- ma, carcinoma simplex, medulary carcinoma and scirrhous carcino- ma, no deletions at these sites were observed in any invasive lobular carcinoma and others. These results imply an etiological difference.P53 gene is a hot point gene in the occurrence and development of breast, cancer. PCR-SSCP analysis was performed to detect P53 gene point mutation in the region between exon 5 and 8, 5 of 12 (41. 6%) stage I breast cancer patients contain mutation of P53, 3 of 5 patients were accompanied by 〓 deletion. These results suggested that point mutation and allelic loss of P53 gene are two vi- tal genetic events in earlier stage of breast tumorigenesis.

我们还发现，〓和〓位点LOH均分布在乳腺浸润性导管癌、单纯癌、髓样癌及硬癌中，在浸润性小叶癌和某些特殊类型乳腺癌中全部为LOH阴性，上述位点LOH可能与某些组织学类型乳腺癌的发生有关。P53基因是乳腺癌形成过程中的一个热点基因，本研究对12例Ⅰ期乳腺癌组织标本中P53基因热点区域第5、6、7、8外显子点突变进行了测定，发现41.6％（5／12）的病人有P53基因一个或多个点突变，其中3例同时伴有〓位点LOH，表明P53基因点突变和等位基因缺失是发生在乳腺癌形成早期的一个重要遗传学事件。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。