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This review describes the research on aphid-resistant genes used in current study and application, and will focus on the expression of Galanthus nivalis Agglutinin gene and Amaranthus caudatus agglutinin gene in transgenic plants and their aphid-resistant effects.

本文讨论了多种抗蚜基因，并重点论述雪花莲凝集素和苋菜凝集素基因在目前科学研究和生产实践的应用。

A peptide antibiotics gene from Amaranthus hypochondriacus, Ah-AMP, was amplified by PCR and cloned. Sequence analysis results revealed that this gene is 261bp in length encoding a precursor polypeptide of 87 amino acid residues.

本文通过PCR技术从苋属植物千穗谷的总DNA中扩增出苋菜多肽抗生素的核基因片段，序列分析结果表明该基因长261bp，编码一个由86个氨基酸组成的Ah-AMP前体多肽。

Transgenic plants expressing GNA showed significant insecticidal activity towards \\omoptera insects such as aphids and brown planthopper in bioassay and feeding tests.In this paper a lectin gene was firstly isolated from leaves of Zephyranthes grandiflora, belonging to Amaryllidaceae, by the use of rapid amplification of cDNA ends technique. Characterization, functional prediction and construction of plant and prokaryotic expression vector of this novel gene were studied.

本实验采用cDNA末端快速扩增(Rapid amplification of eDNA ends，RACE)技术，首次从中国特有的野生植物韭莲(又名风雨花，Zephyranthes grandiflora)的叶片中分离克隆出了一个新的凝集素基因，对基因进行了序列分析和功能预测，并成功地构建了韭莲凝集素基因的植物表达载体和原核表达载体。

Until June 2007, seventy-eight species of Amphibians' complete sequence of mitochondrial genome were presented in GenBank. The gene numbers in the genomes range from 35 to 41; the 78 mtDNA genomes can be divided into 22 types and further clustered as 3 groups based on the gene number, kind and arrangement. The type 4 is a common type among the class Amphibia and other vertebrate, type 8 is common among the three orders of living Amphibia.

对2007年6月13日以前公布于GenBank上的78种两栖动物的线粒体基因组全序列进行了总结、比较和分析。78种基因组中基因的数量从35~41个不等；根据基因的数量、种类及其排列顺序的差别将其分为22种基因组类型，其进而聚为3组，其中类型4为两栖纲与其它脊椎动物的常见类型，类型8为两栖纲中现生3个目的公有类型。

In fish and amphibia, the transition from maternal gene transcription to zygotic transcription happens during the time of midblastula formation, this stage is named by midblastula transition, at this period, embryo cell cycle composition changes, it is the first time to G1 time appearance, the cell cycle timelimit lengthens and the cell division synchronism loses, the large quantities of zygotic gene transcription starts.

鱼类和两栖类由母型调控向合子型调控过渡发生在中囊胚形成阶段，称为中囊胚过渡(midblastula transition，MBT)，胚胎细胞周期时相的组成在此期间发生变化，首次出现G1期，周期时限延长，细胞分裂的同步性丧失，大批合子型基因活化转录也在此时开始。

This study separated high quality of steel and high elastic silk gene of major ampullate gland and flagelliform gland of Aranbeus ventricosus,and saparated mRNA ,and published several new gene of silk protein for AvF1(AY174110),AvF2(AY177203),AvFf1(AY587193) in GeneBank.

通过鸟枪法、Southern杂交等方法筛库，在没有借鉴国外丝蛋白基因的基础上，获得具有自主知识产权的蛛丝蛋白mRNA，并将蛛丝蛋白AvF1(AY174110)、AvF2(AY177203)、AvFf1(AY587193)等多个新基因登录GenBank。

Furthermore antisense plant expression vector of this gene was constructed using the pBI121 vector, laying foundation for studies on antisense regulation of ginseng β-amyrin synthase gene.

在此基础上，利用pBI121质粒载体，构建了人参β香树素合成酶基因的反义植物表达载体，为这一基因的反义调控研究打下基础。

The degree of anabrosis seems to be more severe in Hp with jhp947 gene than in Hp without jhp947 gene.

JHP947基因存在可使Hp感染所致的胃黏膜损伤程度加重。

It is a new way to breed cold tolerant cotton by gene transformation with antifreezing protein gene from desert insect.

利用新疆荒漠昆虫抗冻蛋白基因进行遗传转化是培育新疆棉花抗寒品种的新途径。

This article summarizes the research achievement on the structure of anura mtDNA this year, analyzes the diversity of frogs mtDNA gene rearrangement, we found that there are 8 kinds of different mtDNA arrangement orders altogether, and also preliminarily analyzes the relation of this different gene structure.

本文综述了今年来在无尾目线粒体基因组结构的研究成果，分析了蛙类mtDNA基因重排多样性，结果发现无尾目动物共有8类不同的线粒体基因排列方式，并初步分析了这些不同基因结构的关系。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。