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As a result, Core gene was expressed in E.coli with yields of 20% of total proteins and it was a 31KD fusion protein on SDS-PAGE and Western-Blot, it shows that Core protein was highly expressed in E.coli;One fragment of 369bp could be seen on the gel of the Core gene PCR product. A band of 29KD could be seen on the Western-blot after induction of yeast containing pPICZaA-Core for 72 hours. This indicate that Core protein was secretively expressed in the Pichia pastoris with weak yields.

结果显示pBVIL1-Core的表达产物经SDS-PAGE分析出现一条约31KD的带，与预期融合蛋白的分子量相符，表达蛋白存在于包涵体中且表达量占菌体总蛋白的20%，Western-blot显示诱导后菌体在相应位置出现特异性杂交带，表明

It is possible that one of gene was derived from another gene in the same group by segmental duplications of chromosome.

通过对水稻细胞周期关键调节基因的系统进化树和蛋白质结构分析发现，在水稻和拟南芥中细胞周期关键调节基因之间是非常保守的。

Functinal complementation test showed that trehalose-6-phosphate synthase mutant (tps1△), transformed by the open reading frame of SpTPS1 gene, restored growth on the mediam supplemented with glucose as a sole carbon source. This result indicated that trehalose-6-phosphate synthase gene (SpTPS1) of Selaginella pulvinata has biologically activity and is hopful to be applied to transgeneic improvement of plant for abiotic stress tolerance.

酵母功能互补试验证明，用SpTPS1基因开放阅读框转化的海藻糖合成酶基因突变（tps1△）酵母菌株，可恢复在以葡萄糖作为唯一碳源培养基上的生长，说明垫状巻柏海藻糖-6-磷酸合成酶基因SpTPS1具有生物活性，可能应用于植物抗逆性的转基因改良。

The hereditary behavior of bar gene in T1 and T2 generated by self-fertilization of bar-transgenic wheats-wanmai48 and ymai87158 was studied. Effects on agronomic traits of bar gene were also researched.

以皖麦48和扬麦87158两个小麦品种的转bar基因株系为材料，探讨bar基因在转基因小麦自交后代的遗传表现及对农艺性状的影响。

Objective To explore an experimental method of transfecting the marrow stromal stem cells with the reconstructed PGL3-transforming growth factor-β1 （TGF-β1） gene and to evaluate the feasibility of self-induction of MSCs to the chondrocytes in vitro so as to provide a scientific and experimental basis for a further ＂gene enhanced tissue engineering＂ research.

目的 探讨将编码细胞转化生长因子β1（transforming growth factorβ1，TGF—β1）的重组PGL3-TGF-β1质粒转染兔骨髓基质干细胞（marrow stromal stemceils，MSCs），体外通过自分泌、诱导作用向软骨细胞方向分化的可行性，为基因加强组织工程学修复软骨损伤提供体外实验依据。

The results on the fitness of F1 showed that both of those two kinds of F1 had good germination and growth rate, which was not significantly different from that of wild B. juncea. However the seed setting of F1 with glufosinate-resistant gene and glyphosate-resistant gene were only 0.50 and 0.61 per silique, which decreased significantly compared to that of self-pollination of wild B.

对F1的适合度研究表明，两种F1种子萌发率及营养生长情况和野芥菜没有明显差异，但结实明显下降，携带抗草丁膦基因的F1和携带抗草甘膦基因的F1每个角果饱满种子粒数分别是0.50和0.61。

Loss of expression of Rb gene was found in 3 and mutations in P_(53) gene were identified in 4 cases of seminoma.

本结果提示人睾丸精原细胞瘤的发生与抗癌基因Rb蛋白产物表达的缺失和P_（53）基因的突变有关。

With western blot analysis and DNA polymerase chain reaction-single strand conformation polymorphism assay,protein product of Rb gene and mutation in exon 5～8 of P_(53) gene were examined in 17 cases of human testicular seminoma.

采用Western印迹和DNA聚合酶链反应－单股构造多态性分析法对17例人睾丸精原细胞瘤组织标本的抗癌基因Rb表达的蛋白产物和P_（53）基因外显子5～8的突变进行检测，发现有3例标本的Rb基因表达的蛋白产物缺失，有4例标本的P_（53）基因有点突变，对照的正常人睾丸组织和视网膜组织均呈Rb基因蛋白产物表达阳性，对照的正常人睾丸组织中未发现P_（53）基因突变。

The effects of CBF1 on cold tolerance,growth and development of low-temperature sensitive plant were investigated by delivering Arabidopsis CBF1 gene(cbf1) concatenated with GUS gene into tobacco.

温度是决定植物地域分布的主要限制因子，是在栽培条件下影响着农作物及园艺作物等的产量和品质的主要因素。

Results of gene disruption indicated that sawC is essential to septation during sporulation. Increase of gene dosage of sawC has no obvious effects on morphological differentiation. When sawC was introduced into Streptomyces coelicolor C13 (whiA13), C13 showed phenotype the same as wild strain.

圈卷产色链霉菌中sawC的破坏导致不能形成孢子分隔。sawC基因剂量的增加对形态分化无明显影响。sawC引入天蓝色链霉菌C13(whiA13)中，可使C13恢复野生型表型。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。