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In the new view, viz. Neo-Darwinism theory, product family development system is analyzed and the architecture of product family gene model is built. After analyzing the gene expression of product family model, the center-principle for product family gene model is proposed and its information hereditary mechanisms are clarified. The algorithms of transcription, retrotranscription, translation, direct-translation and mutation for information gene are presented.

从一个新的角度——现代达尔文主义学说对产品族设计系统进行了分析，在此基础上，建立了产品族基因建模体系结构，分析了产品族模型的基因表达，提出了产品族基因建模中心法则，并对其中各个信息遗传机制进行了详细的分析和论述，给出了相应的信息基因转录、逆转录、基因转译、基因直译以及基因突变的算法。

ASCL2 gene escapes genomic imprinting in skeletal muscle,fat, heart,liver,spleen,lung,kidney,stomach,small intestine,uterus,ovary and pituitary.(2) Both of the transcripts of NNAT gene is paternally expressed in skeletal muscle,fat,heart, liver,spleen,lung,kidney,stomach,small intestine,uterus,ovary and pituitary.(3) DIRAS3 gene is paternally expressed in skeletal muscle,fat,heart,liver,spleen,lung, kidney,stomach,small intestine,uterus,ovary and pituitary.(4) DLX5 gene is maternally expressed in skeletal muscle,fat,spleen,lung,stomach,small intestine,but escapes imprinting in heart,liver,kidney,uterus,ovary,testicle and pituitary.

1ASCL2基因在肌肉、脂肪、心、肝、脾、肺、肾、胃、小肠、子宫、卵巢和垂体中均逃脱印记；(2)NNAT基因两个转录本在肌肉、脂肪、心、肝、脾、肺、肾、胃、小肠、子宫、卵巢和垂体中均为母系印记：(3)DIRAS3基因在肌肉、脂肪、心、肝、脾、肺、肾、胃、小肠、子宫、卵巢和垂体中母系印记：(4)DLX5基因在骨骼肌、脂肪、肺、脾、胃和小肠中表达母本来源的等位基因，即表现为父系印记；而在心、肝、肾、子宫、卵巢、睾丸和垂体中表达双亲来源的等位基因，即逃脱印记。3。

The result of sequence analysis indicated that the length of cDNA and DNA fragments of Lectin gene and the length of cDNA and DNA fragments of Pectate Lyase gene were 508 bp,575 bp,1,316 bp and 1,858 bp respectively.The DNA fragment of Lectin gene had a intron,whose length was 67 bp .The DNA fragment of Pectate Lyase gene had 3 introns,whose lengths were 90 bp,83 bp,and 370 bp respectively. All these four introns sequences followed the rule of GT-AG.

通过序列分析得知，香蕉凝集素基因cDNA片段长508 bp；香蕉凝集素基因DNA片段长575bp，有一个长为67bp的内含子；香蕉果胶裂解酶基因cDNA片段长1，316bp；香蕉果胶裂解酶基因DNA片段长1，858bp，有三个内含子区，分别长90 bp，83 bp和370bp；在总共的四个内含子序列中，分别都符合GT-AG规则。

Methods Four pairs of primers were designed from toxin sub-unit A gene, O139 special gene, Haemolytic subunit A gene and Toxin coregulated pilus subunit A gene. Multiplex PCR was established to simultaneously detect those four genes.

分别针对霍乱弧菌肠毒素A亚单位基因、O139群特异基因、霍乱弧菌溶血素A亚单位基因和毒力协同调节菌毛A亚单位基因设计引物，建立多基因PCR方法。

Under different stresses, we tested the expression of citrate synthase gene in rapeseed leaf by using semi-quantitative PCR. The expression of citrate synthase gene had no obvious change in stresses of salt, dark, high illumination, while was increased at different time in treatments of water logging, drought, IAA, and 6-BA. Interestingly the effect of ABA was contrary to that of IAA. In the treatment of sclerotium blight, the expression of citrate synthase gene was depressed. There was a saddle curve of citrate synthase gene expression in the treatment of gibberellin.

对油菜幼苗进行植物生长调节物质、高温和低温、强光照和弱光照、盐、菌核病、干旱和水渍等处理，采用半定量PCR法对油菜叶片柠檬酸合酶基因的表达模式进行检测，发现在盐胁迫、暗光和强光的处理下，柠檬酸合酶基因的表达基本没有变化；在水渍、干旱、IAA和6-BA胁迫下，其表达有所升高，但出现峰值的时间不同，ABA对表达模式的影响与IAA相反，感染菌核病后其表达降低；对GA3的应答呈鞍型。

Therefore, NAG7 gene was a nice candidate of tumor suppresser gene associated with NPC. The mechanism might be that the re-expression of NAG7 gene contributed to the change of gene expression profile and following the change of protein expression profile, then involved in the cell cycle and apoptotic regulation changed, and finally suppressed the NPC growth.

因此，NAG7基因作为鼻咽癌相关的抑瘤基因候选者，其作用机理可能是通过诱发基因表达谱的改变而引起蛋白质表达谱发生相应的改变，在一致性改变的功能分子的作用下，抑制细胞增殖，同时诱发细胞凋亡增加，从而发挥抑制鼻咽癌细胞生长的作用。

Transduction of TNF-α gene into TIL of human glioma by retroviral vectors and study of its biological activity in vitro. The development of retroviral vectors for TNF-α gene transfer and preparation of carrier cells-TIL have al- lowed transducing marked gene NeoR and purpose gene TNF-α into TIL cells respectively by use of monoclone tilter virus PLC-2 and PLJC-5. Detect the proliferation of TIL, expression of TNF-α and the anti-tumor activity of glio- ma TJ8510 cells in vitro.

在TNF-α逆转录病毒转移系统的建立及运载细胞TIL制备的基础上，利用构建的单克隆高滴度病毒细胞株PLC-2和PLJC-5将标记基因NcoR和目的基因TNF-α分别导入到TIL细胞中；对TNF-α基因转导后的TIL细胞进行细胞的增殖情况、TNF-α表达水平及对人脑胶质瘤TJ8510细胞的体外抗瘤活性的检测。

To clone new disease gene of CMT, many myelin and myelin-related gene had been analysised by homologue searching. Quaking mouse, one of the mutation mouse with the clinic feature similar to Trembler mouse (the gene mutation mouse of PMP22) with the characterization of demylination in both the central and the peripheral nervous system, its disease gene had just been cloned accession No.

为进一步克隆新的CMT致病基因，在分析出CMT已克隆的基因均为与髓鞘有关的基因后，通过筛选有关髓鞘基因，我们发现，quaking基因突变小鼠因髓鞘形成障碍而出现震颤，其中枢和周围神经均有脱髓鞘，类似于PMP22基因突变鼠"Trembler"的临床表现。

Known differential expression gene: In the 46 points of known differential expression gene, there were down - regulate gene expression 41 points and up- regulate gene expression 5 points; twenty - eight genes related to hypoxia/reoxygenation had not been reported and 18 genes had been reported.

已知差异表达基因：在46个已知差异表达基因中，表达下调的基因有41个，表达上调的基因有5个，未见报道的与缺氧/复氧处理相关基因23个，已报道的有18个。

The interaction between M. grisea and rice is based on the gene-for-gene hypothesis and the defense responses are often activated by the action of the pathogen avirulence gene and the host resistance gene.

稻瘟病菌与水稻的互作符合经典的基因对基因学说，二者的不亲和互作是无毒基因与抗病基因相互作用的结果。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。