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The Cyt b gene of Takifugu fasciatus was proved to have good homology with other 13 species of fishes recorded in GenBank and it has 96.1% homology with Takifugu rubripes, about 75% with Mola mola and Masturus lanceolatus. The tRNA gene has 69 nucleotides and the tRNA has 72 nucleotides. Their sequences are the same as those of Takifugu rubripes.

用DNA分析软件对暗纹东方鲀与GenBank中10个目13种鱼类的Cyt b序列进行比较分析，显示暗纹东方鲀与这些鱼类的Cyt b基因具有较高的同源性，与同属红鳍东方鲀的同源性最高，为96.1%；与同目不同科的矛尾翻车鲀和翻车鲀的同源性分别为74.1%和74.8%。tRNA基因由69个碱基组成，tRNA基因由72个碱基组成，与红鳍东方鲀相应tRNA的碱基组成完全相同。

Thus, each cell population displays a vast heterogeneity in patterns of mono- and biallelic gene expression, providing numerous combinatorial patterns of gene expression.

因此，每个细胞群在单一和等位基因的表达模式上显示出巨大的差异性，提供了基因表达的许多组合模式。

This promoter was used to construct an induction expression vector for monocotyledon (pC1300WDREB) with transcription factor gene of stress resistance and hygromycin phosphostransferas gene.

用该启动子与抗逆相关转录因子基因和潮霉素磷酸转移酶标记基因一起，构建成单子叶植物诱导表达载体pC1300WDREB。

In order to evaluate the effect of over-expressed Mn-superoxide dismutase gene on antioxydation in maize, expression vector with Mnsod gene from wheat was constructed for monocotyledonous plant and used to transform calli of elite maize inbred line by microprojectile bombardment.

为研究过量表达的超氧化物歧化酶基因对提高玉米抗氧化损伤的作用，本研究构建了小麦来源Mnsod基因的单子叶植物表达载体，用基因枪法转化用优良玉米自交系诱导的胚性愈伤组织。

Genetic analysis showed the dwarf is controlled by a monogenic recessive gene. We named the dwarf gene Ds.

遗传分析表明，该矮秆性状受隐性单基因控制，我们将其暂定名为Ds。

PCR-RFLP in FSHR gene 5'–flanking region with exon 1 digested by restriction endonuclease TaqⅠdemonstrated that the frequencies of allele A (0.5500) and genotype AA (0.5000) in twinning cows were higher than that of allele A (0.3500) and genotype AA (0.3000) in monovular cows for Qinchuan cattle, while The frequencies of allele B (0.5937) and genotype BB(0.6875) in twinning cows were higher than that of allele B (0.5416) and genotype BB (0.4167) in monovular cows for Holstein cattle. Key words: Bovine; Twinning; FSHR gene; PCR-RFLP

FSHR基因5'端（包括侧翼序列和第一外显子）的限制性内切酶TaqⅠ的PCR-RFLP标记研究发现，在秦川牛中双胎母牛的A基因频率（0.5500）和AA基因型频率（0.5000）分别高于单胎母牛的A基因频率（0.3500）和AA基因型频率（0.3000）；在黑白花奶牛中双胎母牛的B基因频率（0.5937）和BB基因型频率（0.6875）均高于单胎母牛的B基因频率（0.5416）和BB基因型频率（0.4167），也就是FSHR基因的5'端的PCR-RFLP标记在一个品种内单胎母牛和双胎母牛之间有一定趋势，但两个品种的趋势不同。

The I/D poly- morphism in the ACE gene is associated with AF in patients with hypertensive LVH. Individuals carrying the DD genotype of ACE gene are predisposed to develop AF.

ACE基因I/D多态与高血压 LVH患者AF的发生显著相关，DD基因型增加AF发生的风险。

Polymorphism of Rb (intron 17) is relatively stable and rarely mutates, but poly morphism of Rb gene (intron 20) locus is characteristic in the old people. The r elation between Rb gene (intron 20) and ageing needs further study.

老年组Rb基因20内含子的位点多态性与青年组比较差异有显著性意义，是否该位点遗传标记与细胞的衰老有一定的相关性，值得进一步研究。

A pair of primers for amplification of σC gene was designed and synthesized according to the published Muscovy duck reovirus S4 gene sequence. The specific RT-PCR product was success-fully obtained and cloned from the Muscovy duck reovirus ZJ99 strain.

参考已发表的番鸭呼肠孤病毒的S4序列设计了1对扩增σC蛋白基因的引物，对mDRV ZJ99株的RNA抽提物进行RT-PCR扩增，获得了特异性的扩增片段。

Hexon protein gene of egg drop syndrome virus, named strain E05 which was isolated from breeding muscovy duck with irregular egg dropping was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmids were identified by digested restriction endonuclease and PCR. The result showed that the nucleotide length of hexon protein gene encoding 910 amino acids was 2733 bp.

运用PCR技术从表现产蛋异常的种番鸭分离鉴定的减蛋综合征病毒 E05株中分段扩增六邻体蛋白基因，分别与克隆载体pMD18-T连接后，转化感受态细胞，对经PCR鉴定和酶切鉴定均为阳性的克隆进行测序，结果表明，E05株六邻体蛋白基因长度为2733 bp，共编码910个氨基酸。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。