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The expression absence of LRRC4 was ascribed to the loss of homozygosity of 7q32-ter in U251 cell lines. Conclusion The expression of LRRC4 gene is absent in glioblastoma cell lines, and it offers the important experiment proof for LRRC4 to act as a new candidate of brain tumor suppressor gene from glioma.

通过对胶质瘤细胞系基因组DNA中LRRC4的ORF序列的PCR扩增和测序分析，发现SF126，SF767，M17细胞中，ORF序列第279位氨基酸的第3个密码子存在同义点突变(3/5)，而U251和U87细胞基因组DNA中发生了LRRC4基因的缺失突变(2/5)。

The concept of cell differentiation; tissue-specific genes; and house-keeping gene; combinational control arouse expression of tissue-specific gene; the factors which have influence on cell differentiation; totipotency; cell differentiation and embryo development.

细胞分化的基本概念；组织特异性基因与管家基因；组合调控引发组织特异性基因的表达；影响细胞分化的因素；细胞的全能性；细胞分化与胚胎发育。

Quantitative method for mRNA level of IBP: The standard preparation of objective gene IBP and house-keeping geneβ-actin were used to get standard curve of real time PCR.

mRNA水平定量方法：利用IBP和管家基因β-actin的质粒标准品绘制real time PCR标准曲线，建立检测IBP的real time PCR标准曲线定量法。

In this paper, three gene S-adenosylmethionine decarboxylase, ornithine aminotransferase and betaine aldehyde dehydrogenase plus one house-keeping gene had been cloned and their expression levels under different stresses were evaluated by real-time PCR technique.

已克隆S-腺苷蛋氨酸脱羧酶、鸟氨酸氨基转移酶、甜菜碱脱氢酶3个基因和内参基因的基础上，应用定量PCR技术，对这3个基因在不同胁迫时间下的表达水平进行分析。

Combined with animal model of peradaptation to myocardium ischemia , the research observed the level of enzymatic dynamics of myocardium, SOD,MDA,ET and correlation peptide of calcitonin gene in peripheral blood and cardiac function, apoptic cell and expression of apoptosis regulatory gene, ultrastructure of myocardium with BIOPAC16, in situ end labeling and hybridism, optic microscope and electron microscope. The study of animal model of peradaptation to myocardium ischemia illustrated that the peradaptation and pretreatment with Wenxin capsule could be positively protective for the injury of ischemia reperfusion. The protection is mainly reflected by reduction of infarction area, correction of heart rhythm abnormality of ischemia reperfusion, melioration of the contraction and relaxation function of myocardium, recovery of the subcellular structure and themelioration of the abnormality of biochemical and bioactive substance in the myocardium.

并结合心肌缺血预适应动物模型，采用BIOPAC16导生理记录和分析处理系统处理心肌功能指标，采用原位末端标记及原位杂交技术标记凋亡细胞及凋亡相关调控基因的表达，采用光电镜技术观察心肌超微结构的变化，同时观察外周血心肌酶、超氧化物歧化酶、丙二醛、内皮素、降钙素基因相关肽等水平，结果表明心肌缺血预适应及温心胶囊预处理对心肌缺血再灌注损伤具有肯定的保护作用，其保护心脏作用主要表现在缩小心肌梗死范围、抗缺血再灌注心律失常、改善心肌收缩和舒张功能、减轻心肌超微结构损伤，调节心肌生化物质及生物活性物质的生成和释放。

DNA from a particular gene will hybridize to that gene's spot on the chip.

来自于一个特殊基因的DNA将与芯片上代表那一基因的点发生杂交。

Timely T4 substitution therapy could strengthen Bcl-2 gene and reduce Bax gene expression and restrained PCD. So it was an important way to correct hypothyroid brain damage.

及时T4替代治疗可以增强Bcl-2并降低Bax基因的表达，有抑制细胞凋亡的作用，是纠正甲减性脑损害的重要方法。

To establish a series of monoclonal antibodies for specific gene products of H-2 complex will not only provide probes for the research on gene constitution of H-2 complex, but also will provide effective genetic quality control measures for the international standardization of laboratory animals in China. It also can offer essential conditions for sophisticated research works in life science field, such as immunogenetics, organ transplantation, etc.

建立一系列H-2特异性基因产物的单克隆抗体，能为研究实验小鼠H-2复合体基因组成提供探针，为我国实验动物的国际标准化提供有效的遗传质量控制手段，也能为免疫遗传学、器官移植等生命科学领域内的尖端研究提供实验室工作方面的重要条件。

There is some obvious inaccuracy of gene expression in the experiment to obtain the gene expression data.

在基因表达谱数据获取过程中，基因表达谱数据含有较大的实验误差。

Methylation of promoter in p16 gene is one of the important mechanisms of the inactivation of the gene.

喉癌中p16基因的甲基化是该基因失活的重要机制之一，p16基因的失活与喉癌的发展和演进密切相关。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。