查询词典 gene

BACKGROUND: Many studies show that ectogenic human platelet-derived growth factor B gene transfection can effectively promote the wound healing.

背景：许多研究显示外源性的人血小板源性生长B基因的转染可促进创伤的愈合。

BACKGROUND: Many studies show that ectogenic human platelet-derived growth factor B gene transfection can effectively promote the wound healing.

摘要背景：许多研究显示外源性的人血小板源性生长B基因的转染可促进创伤的愈合。

The ectogenous gene TSLC1 has integrated into the genomes of T_3B cells which wereidentified from both nucleic acid and protein levels.

结果：(1)成功获得高表达TSLC1的稳定细胞系，核酸和蛋白水平鉴定都说明TSLC1基因成功整合到细胞基因组中。

Specific gene expression of the pro-inflammatory cytokine IL-1β and the type II IL-1βreceptor (IL-1RII, 'decoy receptor') was studied in skin of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) fry during infection with the ectoparasitic monogenean Gyrodactylus derjaini Mikailo, 1975. Cytokine expression was studied in both primary and secondary infections using a semi-quantitatie RT–PCR technique.

用半定量RT-PCR的方法，研究初次感染和二次感染体外寄生的单殖吸虫---三代虫后，虹鳟鱼苗的皮肤中促炎症细胞因子IL-1β及IL-1βII型受体（IL-1RII）的特异性基因表达。

Specific gene expression of the pro-inflammatory cytokine IL-1β and the type II IL-1βreceptor (IL-1RII, 'decoy receptor') was studied in skin of rainbow trout Oncorhynchus mykiss (Walbaum, 1792) fry during infection with the ectoparasitic monogenean Gyrodactylus derjavini Mikailov, 1975. Cytokine expression was studied in both primary and secondary infections using a semi-quantitative RT–PCR technique.

用半定量RT-PCR的方法，研究初次感染和二次感染体外寄生的单殖吸虫---三代虫后，虹鳟鱼苗的皮肤中促炎症细胞因子IL-1β及IL-1βII型受体（IL-1RII）的特异性基因表达。

Key words: Eimeria acervulina;3-1E gene;eukaryotic expression plasmid;immune protection

堆形艾美球虫；3-1E基因；真核表达质粒；免疫保护

Eimeria maxima ; gam56 gene ; recombinant protein ; protective effect

巨型艾美球虫； gam56基因；重组蛋白；保护效果

Methods The promoter of hTERT and Cox-2 was subcloned from human genomic DNA, then ligated to shuttle vector pd306 which contains the Ela and Elb gene of adenovirus to control the expression of Ela and Elb,respectively.

将hTERT和Cox-2启动子从人类白细胞基因组中亚克隆出来，并将启动子分别插入到腺病毒穿梭载体pd306上的E1A和E1B基因前，使hTERT和Cox-2启动子分别调控腺病毒必须基因E1A和E1B的表达，再将构建后的pd306和腺病毒的骨架质粒BHGE3在Ad293细胞内进行同源重组，并用重组后的病毒感染Hela细胞检测病毒对肿瘤细胞的杀伤力。

The PCR product were detected by agirose and polyaridlamid gel electrophore- sis for quentifying Myc gene. The tissue samples were same as former. The C—myc amglification appersed 42% in larynx cancer and 20% in djacent tissue.

发现喉癌组织C—myc扩增率为42％，癌旁组织扩增率为20％。C—myc的扩增多发生在低分化癌中，提示肿瘤分化和C—myc之间存在着一定的相关性。

Methods The plasmid pCEP4P53 expressing human p53 protein and the plasmid pCEP4ASCMH expressing human antisense c - myc gene in eukaryocyte were co - transfected to human bladder cancer cell T24 strain using FuGENE6 as a media, and the results were analyzed by MTT method, cell colony - forming test in soft agar medium and agarose gel electrophore...

将可在真核细胞表达P53蛋白的质粒pCEP4P53和表达反义c-myc基因的质粒pGEP4ASCMHC以染试剂Fu-GENE6为介导，同时转染到入膀胱癌细胞T24中，经MTT检测、软琼脂集落生长、琼脂糖电泳等方法分析实验结果。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48